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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The colorimetric reaction was then measured at 540 nm using a Multiskan Go microplate reader (Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... IL-1β ELISA kit (Catalog # 88-7013-22) and TNFα ELISA kit (Catalog # 88-7324-88) were from Invitrogen (America). LDH Cytotoxicity Assay Kit (C00170 ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... cells were first synchronized overnight in 1% FCS DMEM and then cultured for 14 days in 10% FCS DMEM and stimulated daily with IL-1β (1 ng/ml; Invitrogen) or IL-17 at (2.5 ng/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell culture supernatant was collected and used for quantifying IL-8 secretion using the ELISA assay kit (# 88-8086-88, ThermoFisher). The assay was performed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... anti-rabbit IgG-Alexa Fluor 488 (R37116, RRID:AB_2556544) and anti-chicken IgG-Alexa Fluor 594 (A11042, RRID:AB_142803) (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Immunology 2021Quote: - IFNγ and IL-10 levels were measured in the supernatant of the WBA using IFN gamma and IL-10 Human ELISA Kit (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2021Quote: ... and protein disulfide bonds were reduced using 10mM dithiothreitol and alkylated with 25mM iodoacetamide (Thermo Fisher Scientific, Rockford, IL, USA). Proteins were then deglycosylated with PNGaseF (New England Biolabs ...
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bioRxiv - Physiology 2021Quote: ... Peptide concentration was measured with a colorimetric assay (Pierce Quantitative Colorimetric Peptide Assay kit, Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Physiology 2021Quote: ... and the Y2H library screening was performed using the GAL4-base system following the manufacturer’s instruction (Thermo Scientific, Rockford, IL). The bait vector pDEST22-CaPti1 was co-transformed into yeast strain MaV203 with the prey vector pDEST32-CaERF4 ...
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bioRxiv - Physiology 2021Quote: ... The concentration of PGE2 was normalized by total protein concentration using a BCA assay (#23225, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 0.1% Triton X-100 (Fisher, Waltham, MA, USA) and Halt protease inhibitors (Pierce Thermo Fisher, Rockford, IL, USA) followed by rocking for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... DF-1 cells were dissociated and counted using a hemacytometer (1492, Hausser Scientific Partnership, Fisher Scientific, Hanover Park, IL, USA) (Tyler and Hall ...
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bioRxiv - Biophysics 2021Quote: ... was thawed at RT and 100 μl added to 10 ml of Middlebrook 7H9 media (Fisher Scientific, Hanover Park, IL), warmed to 37° C ...
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bioRxiv - Immunology 2020Quote: Cytokines (IL-5 and IL-13) in supernatant of human and murine ILC2 cell cultures were analyzed with ELISA kits from Invitrogen. For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend ...
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Immunology 2020Quote: ... antibodies were buffer exchanged from Tris buffer to PBS buffer using the Zeba spin protocol (ThermoFisher Scientific, Rockford, IL, USA), as amine-containing buffers may interfere with biotinylation ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Immunology 2020Quote: ... submandibular bleeding was performed to collect blood for measurement of serum IL-1β levels by ELISA (Invitrogen 88-7013-22). At 4 hours ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... and dialyzed overnight against water using 3.5 kDa molecular weight cut off Snake Skin dialysis tubing (Thermo Scientific, IL, USA). Dialyzed filtrate was loaded onto a HiPrep SP XL 16/10 cation exchange column (GE Healthcare Piscataway ...
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bioRxiv - Microbiology 2022Quote: ... To measure the protein concentration of IFN-λ1 and -λ2 present in the SN we performed an ELISA following the protocol from the manufacturer: IFN-λ1 (IL-29) from Invitrogen/Thermo Fisher Scientific cat ...
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bioRxiv - Neuroscience 2022Quote: CeA punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: PER2::LUC rhythms were monitored for 42 hours prior to the addition of 10 ng/mL of IL-1α (R&D) or matching vehicle (UltraPure DNase/RNase-Free Distilled Water, Invitrogen). Explants were heat shocked immediately or three hours after IL-1α addition.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cell culture supernatants were collected and analyzed for IL-8 release using a commercially available ELISA kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Bioengineering 2024Quote: ... the amount of IL-10 in the HDL-rich fraction and the (V)LDL-rich fraction was quantified with an IL-10 ELISA (Invitrogen) and used to calculate the fraction of bound IL-10 ...
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bioRxiv - Bioengineering 2024Quote: ... the fluorescence intensity of the stained IFNγ and IL-2 antibodies was quantified using an Attune NxT Flow Cytometer equipped with an autosampler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein content of the resulting lysate was quantified using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final) (Thermo Scientific, Rockford, IL, USA) to the M-PER buffer ...
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bioRxiv - Microbiology 2023Quote: The MMTV sag gene 3’ region was derived from IL-10-/-spleen RNA by conversion to complementary DNA using a SuperScript IV Reverse Transcriptase kit (Invitrogen), and PCR amplification with two separate primer sets ...
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bioRxiv - Neuroscience 2023Quote: ... Lateral striatum was dissected from each hemi-section and placed in HABG media (Hibernate A Media, BrainBits, Springfield, IL, HA500; 2% B27 Supplement, ThermoFisher, Cat #17504044 ...
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bioRxiv - Cell Biology 2023Quote: ... Amplification of the cDNA samples was carried out using PowerUpTM SYBRTM Green Mastermix (Thermo Fisher Scientific-A25742, Rockford, IL, USA). The real-time PCR reactions were performed using the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Microbiology 2023Quote: ... at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific, Rockford, IL, USA) at the Life Science Computer Cluster (LiSC ...
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bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... The LC-MS/MS analysis was performed using an LTQ Orbitrap Elite mass spectrometer (Thermo Scientific, Rockford, IL Waltham, MA) coupled online to an Easy-nLC 1000 in the data-dependent mode ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher, Rockford, IL, USA). The conditioned media (1.8 ml ...
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bioRxiv - Immunology 2023Quote: ... and complementary DNA (cDNA) was prepared by using Maxima™ H Minus cDNA Synthesis Master Mix (Thermo Scientific, Rockford, IL). Real-time qPCR (qRT-PCR ...
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bioRxiv - Immunology 2022Quote: IFNγ mRNA and protein were measured in IL-2 expanded MAIT cells (with or without stimulation with CD3/CD28 Dynabeads (Gibco)) and their culture supernatant by qPCR or ELISA respectively ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration in the supernatant was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... protein concentration of the CF was determined using the Coomassie (Bradford) Protein assay Kit (Thermo Fisher Scientific, Rockford, IL, USA) and bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2023Quote: ... the total protein concentration was determined using the bicinchoninic acid (BCA) protein assay kit (Pierce/Thermo Fisher Scientific; Rockford, IL). 25µg of protein samples were mixed with 4x LDS sample buffer (Life Technologies ...
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bioRxiv - Immunology 2024Quote: Cell culture supernatants collected at various time intervals from in vitro stimulated T cells of mice or humans were analyzed for IL-2 by ELISA using kits purchased from ThermoFisher Scientific (mouse IL-2 ELISA kit ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The membranes were then treated with secondary antibodies (biotinylated goat anti-rabbit IgG (#65-6140, Invitrogen, Rockford, IL, United States), biotinylated goat anti-rat IgG (#405428 ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression levels were measured by quantitative real-time PCR using the SYBR Green assay (Thermo Scientific, Rockford, IL, USA)on a StepOne instrument (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... in a final volume of 10□µl using an ABI Prism 7000 Sequence Detection System (Thermo Scientific, Rockford, IL, USA). The PCR reactions were initiated with a 10-min incubation at 95□°C followed by 40 cycles of 95□°C for 15□s and 60□°C for 60□s ...