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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... in 5% FBS with 1mM sodium pyruvate (Thermo Fisher Scientific, Waltham, MA, USA) with no antibiotics ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 μg/ml of Alexa546 or 647-conjugated fibrinogen (Thermo Fisher Scientific) in 100 mM NaHCO3 at pH 8.6 ...
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bioRxiv - Biophysics 2021Quote: ... The filtered lysate was passed through 5 mL Ni-NTA agarose (Thermo Fisher), followed by typically 3 washes with 5 column volumes of wash buffer (20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked for 2 hr with 5% goat serum (Thermo Fisher Scientific) and 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 100 μl PBS containing 5 μM CFSE (Molecular Probes, C-1157), incubated for 5 minutes at RT ...
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bioRxiv - Cell Biology 2021Quote: ... A 5 fold-excess of Alexa Fluor 488 C5-maleimide (Thermo Fisher Scientific) was added overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and then amplified on the QuantStudio 5 Real Time PCR System (Applied Biosystems). Specific primers designed to amplify the desired gene were combined with cDNA and Platinum® SYBR® Green qPCR SuperMix-UDG (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... SeeBlue™ Plus2 Pre-stained Protein Standard (5 μl, Thermo Fisher Scientific, LC5925) was used as a standard ...
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bioRxiv - Cell Biology 2022Quote: ... and 5 µL of Dynabeads MyOne Silane magnetic beads (Thermo Fisher cat#37002D) were dispensed into each well using the repeater pipette ...
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bioRxiv - Cell Biology 2022Quote: ... neutrophils were loaded with a morphological tracer Cell tracker red (5 μM, Invitrogen) and Ca2+-indicator Fluo-4/AM (5 μM ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL SYBR Green mastermix (Applied Biosystems by Thermo Fisher Scientific, Ref. #4344463) and 0.5 μL of 2 μM of each corresponding forward and reverse qPCR primers ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were stained with .1μg/mL DAPI for 5 mins (Thermo Fisher) and mounted on the slides using Fluoromount (Southern-Biotech) ...
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bioRxiv - Immunology 2022Quote: ... followed by incubation for 8 min using 5 μM CellTrace Violet dye (Invitrogen). Following fluorescent labeling ...
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bioRxiv - Molecular Biology 2022Quote: ... Antibodies (5 µg each) used for RIP were: Rabbit IgG (Invitrogen, #02-6102), Fibrillarin (Abcam ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were reduced with 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher) for 30 min and alkylated with 10 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF10A cells were maintained in DMEM:F12 media supplemented with 5% horse serum (Gibco), 10 μg/mL insulin (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were scraped into DMEM containing 5% FBS and 15 mM HEPES (Gibco) and centrifuged at 300 g for 10 minutes at 4℃ ...
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bioRxiv - Physiology 2022Quote: ... and 5 % (v/v) charcoal-stripped fetal bovine serum (Thermo Fisher Scientific, #A3382101)) ...
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bioRxiv - Immunology 2022Quote: ... containing 5% FBS (Capricorn Scientific, Ebsdorfergrund, Germany) and 86 µg/ml gentamicin (Gibco) without or supplemented with 20 µg/ml polyinosinic:polycytidylic acid (poly(I:C) ...
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bioRxiv - Pathology 2022Quote: ... Expression was measured in a Quant-Studio 5 RealTime PCR system (Thermo Fisher, 2 min 50 °C ...
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bioRxiv - Microbiology 2022Quote: ... respectively) and 5 μl of 4X TaqMan Fast Virus 1-step mastermix (ThermoFisher). The qPCR plate was sealed and read in the FAM and HEX channels using a QuantStudio™ 5 Real-Time PCR system (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... stained for nuclei (DAPI – ThermoFisher Scientific 62248 – 1:2000 for 5 min RT) and mounted with Fluoromount G (ThermoFisher 00-4958-02) ...
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bioRxiv - Microbiology 2022Quote: Vero cells were cultured for 5−8 passages in DMEM medium (Gibco, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Developmental Biology 2022Quote: ... day 5 expanding VFG cells were treated with DMEM GlutaMAX medium (Thermo Fisher) with 1% vol/vol B27 supplement (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... The peptide was resuspended in PBS and labelled with Fluorescein-5-Maleimide (ThermoFisher), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and used in a 50μl transcription reaction containing 5 x transcription buffer (ThermoFisher), 8mM rNTPs ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-(and-6)-chloromethyl-2′,7′ dicholorodihydrofluorescein diacetate (CM-H2DCFDA; Molecular Probes C6827), was used to visualize ROS accumulation (excitation ...
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bioRxiv - Immunology 2022Quote: 5 × 106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10 min ...
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bioRxiv - Immunology 2022Quote: ... 5×106 MC/9 cells were suspended in 0.5 ml of optiMEM (GIBCO) and kept at room temperature for 10min before electroporation was performed as stated above.
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bioRxiv - Immunology 2022Quote: ... or 2.5μM of 5-(and-6)-Carboxy-2’,7’-Dichlorofluorescein Diacetate (DCFDA) (Invitrogen) was then added and incubated with the cells 20 minutes at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... and H1-G2E1-5 was detected by mouse anti-His6-tag antibody (Invitrogen) and subsequently by HRP-conjugated secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Genomics 2022Quote: ... The erythrocytes were lysed using 5 mL ACK lysing buffer (Gibco, Shanghai, China). Dead cells were removed with a Dead Cell Removal Kit (Miltenyi ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 cells were stained with 5 µM MitoSOX (Molecular Probes, Thermo Fisher) in PBS at 37°C for 40 min ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 × 106 cells were stained with 5 µM MitoSOX (Molecular Probes, Thermo Fisher) in PBS at 37°C for 40 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-10 μg of protein were boiled in NuPAGE LDS sample buffer (Invitrogen) at 95°C for 5 min and separated using NuPAGE 4%–12% Bis-Tris Protein Gel (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking for 2 hours in 5% goat serum (Invitrogen, Waltham, MA) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... or a control mimic at 5 nM final concentration using Opti-MEM (Gibco) and Lipofectamine RNAiMax Transfection reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time qPCR was performed using QuantStudio 5 Real-Time PCR System (ThermoFisher) and PowerUp SYBR™ green master mix ...
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bioRxiv - Neuroscience 2021Quote: Samples from 5-month-old SNR-derived organoids were homogenized in Trizol (Invitrogen), and total RNA was purified using the RNeasy Micro Kit (Qiagen ...
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bioRxiv - Neuroscience 2020Quote: ... and were loaded together with 5 μL of loading buffer (Invitrogen, Germany, EU) into a 12% polyacrylamide gel.
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bioRxiv - Neuroscience 2020Quote: ... The tissue was dissociated into cells in DMEM containing 5% FBS (Gibco, 1008214) and 1% Penicillin/Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in groups of 5/well using 4-well plates (ThermoFisher). Individual HIO lumens were microinjected using a glass caliber needle with 1μl of PBS control or different STm mutants (105CFU/HIO or 103CFU/HIO for 24h infections) ...
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bioRxiv - Microbiology 2020Quote: ... and reactions performed on the QuantStudio 5 Real-Time PCR systems (Thermo Fisher). Host gene expression was determined using the 2-ΔΔCt method and normalised to GAPDH expression ...
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bioRxiv - Cancer Biology 2020Quote: ... OSI-027 (5 μM, 10 μM, 25 μM, and 100 μM) (Fisher Scientific), and Everolimus (2 nM ...
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bioRxiv - Immunology 2021Quote: ... for 20 min or 5 µM MitoSOX™ Red mitochondrial superoxide indicator (Invitrogen) for 10 min at 37 °C according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: 5 × 15cm plates of HEK293 Flp-In T-REx cells (ThermoFisher Scientific, R78007) at 80% confluency were harvested in 15mL DMEM media by trypsinization ...
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bioRxiv - Microbiology 2021Quote: ... in a humidified incubator supplemented with 5% CO2 at 37°C (Thermo Scientific). HepAD38 cells were cultured as previously described26 ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were stained with the DNA-binding dye Hoechst (5 μg/ml; Invitrogen), and coverslips were mounted in mounting medium (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies: Claudin-5 (mouse, clone 4C3C2, ThermoFisher cat# 35-2500, 1:100), Iba1 (goat polyclonal ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a short 5 min incubation in a DAPI solution (Molecular Probes). A 5 min incubation in 1% Sudan Black B prepared in 70% ethanol was applied to quench autofluorescence ...