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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for ssc mir 17 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with AgPath-ID One-Step RT-PCR reagents (Thermo Fisher Scientific), panRusV-2 primers (final concentration ...
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bioRxiv - Genomics 2022Quote: RNA was reverse transcribed for 20 min at 54°C with 1.5 µL RT primer and 10 µl RT mix (0.6 µl Superscript III (Thermo Fisher cat# EP1756B012), 2.17x SuperScript III RT buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Methylated RNA species were recovered from the elution and reverse transcribed (RT) using the RT primer and SuperScript III (Invitrogen 18080044). Excessive RT primer was digested with ExoSAP-IT (Applied Biosystems 78250) ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers for qRT-PCR were purchased from Life Technologies: Arg1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Custom PCR primers (DNA) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was reaction mixture was set up using TaqMan Fast Advanced Master Mix (Thermo Fisher) and Taqman probes for POLDIP2 (Hs00210257_m1 ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative reverse transcription-PCR (qRT-PCR) was performed using the StepOnePlus RT-PCR system (Applied Biosystems) and the 5x HOT FIREPol® EvaGreen® qPCR Supermix kit from Solis BioDyne ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by two final washes in 5X SSCT (20X SSC from Thermofisher; SSCT is SSC with 0.1% Tween20) for 5 minutes each ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-Time PCR was performed for miR-4521 expression analysis using miRNA Taqman assay (Applied Biosystems, USA) and gene expression analysis using PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... specific mRNA or miR-143/145 was amplified using Power SYBR Green PCR Master Mix (Applied Biosystems) or Taqman Universal PCR Master Mix ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative RT-PCR was carried out using a PCR system (StepOne; Applied Biosystems) and the Luna One-Step RT-qPCR Kit (NEB ...
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bioRxiv - Evolutionary Biology 2021Quote: ... CD33m was amplified by PCR using SuperScript III One-Step RT-PCR (Invitrogen) and Gene-specific primers 5’-TTATATGCTAGCGCCACCATGCCGCTGCTGCTACTGCTGC-3’ ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-qPCR PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems) using primers listed in Table 1 ...
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bioRxiv - Cancer Biology 2019Quote: ... RT–PCR was done using SYBR Green PCR Master Mix (Applied Biosystems, 4309155) on a ViiA 7 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed on StepOne® Realtime PCR system (Applied Biosystems) using KAPA SYBR FAST qPCR Kits (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... RT-PCR was performed on a 7500 Real-Time PCR System (Applied Biosystems) using the iTaq Universal SYBR Green Supermix (BioRad) ...
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bioRxiv - Immunology 2022Quote: ... followed by RT-PCR with Fast SYBR Green PCR Master Mix (Applied Biosystems) in QuantStudio3 (Applied Bioscience) ...
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bioRxiv - Physiology 2021Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using Taqman Fast Advanced Master mix (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... PCR was performed using one step SuperScriptR RT-PCR kit (Invitrogen, Carlsbad, CA) with E7 specific primers ...
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bioRxiv - Plant Biology 2022Quote: ... RT-PCR was performed using Dream-Taq Green PCR master mix (Thermo Fisher). The RT-PCR was performed to validate the expression of full-length Cas12f and tracrRNA-crRNA in the plant ...
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bioRxiv - Physiology 2022Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was carried out using Sybr Green RT-PCR kits (Invitrogen) on a 7900HT fast real-time PCR system (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... Real-time PCR (RT-PCR) was performed on a Quantstudio 3 (ThermoFisher Scientific) using either Luna Universal qPCR master mix for Sybr Green primers (New England Biolabs ...
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bioRxiv - Systems Biology 2021Quote: The V1–V2 region of 16S rRNA was amplified by RT-PCR using the primers PGM- 27F (CCATCTCATCCCTGCGTGTCTCCGACTCAG-[MID]- GATAGAGTTTGATCMTGGCTCAG) and PGM-338R (CCTCTCTATGGGCAGTCGGTGATTGCTGCCTCCCGTAGGAGT) and sequenced using IonPGM (Thermo Fisher Scientific, Waltham, MA, USA) to profile bacterial community structure ...
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bioRxiv - Cancer Biology 2020Quote: ... and 2 μg of total RNA from each sample was used for reverse transcription-polymerase chain reaction (RT-PCR) with random hexamer primers (Invitrogen, Waltham, MA, USA; Cat#58875). SYBR® Green (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... amplicons were produced with primers (WU-21-F: AATCTATCAGGCCGGTAGCAC; WU-86-R: CAACAGCTATTCCAGTTAAAGCAC) using SuperScript IV One-step RT-PCR (Thermo Fisher Scientific; Waltham, MA USA). Amplicons were sequenced on a MinION system using from Oxford Nanopore using Native Barcoding 1-12 (EXP-NBD104 ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative RT-PCR was performed using gene-specific primers (see Supplemental Table S2 for details) and SYBR Green master mix (Thermo Fisher Scientific, Waltham, MA, USA). Negative delta cycle threshold (-Δ Ct ...
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bioRxiv - Bioengineering 2023Quote: ... reverse transcription employed the ThermoFisher TaqMan MicroRNA Reverse Transcription Kit with miR-specific primers and probes (Thermo Fisher Scientific, Waltham, MA, USA), and proceeded according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... 100 ng of RNA and miR specific primers were used for cDNA synthesis using the TaqMan MicroRNA Reverse Transcription kit (Applied Biosystems, 4366597). MiR-541-3p levels were quantified using the Ct method after normalization with RNU44 or U6 and are presented as arbitrary units.
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bioRxiv - Microbiology 2021Quote: ... 200 pM 515F primer and 200 pM barcoded 806R primer (IDT) in PCR-clean water (Invitrogen Ultra Pure DNase/RNase-Free Distilled water) ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic PCR primers were designed using the Primer-BLAST tool (80) and ordered from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed with 0.2x SSC (GIBCO) and PBST ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted in 0.5X SSC (NaCl - Fisher Scientific S271 and sodium citrate - Fisher Scientific S279) ...
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bioRxiv - Systems Biology 2024Quote: ... 2x SSC and 10% formamide (Invitrogen, AM9342). The hybridization of the probes was performed overnight at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... TaqMan real-time RT-PCR was conducted using AgPath-ID™ One-step RT-PCR Reagents (Applied Biosystems, Foster City, CA, USA) and performed on an Mx3000P QPCR System (Stratagene ...
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bioRxiv - Neuroscience 2020Quote: ... The viral RNA loads were determined by real time RT-PCR with the Taqman one-step RT-PCR master kit (Applied Biosystems, CA). The following specific primers and probe of NCSU1 FIV gag region were used ...
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bioRxiv - Neuroscience 2020Quote: ... Quantitative real-time RT-PCR (RT-qPCR) was performed using EvaGreen (Solis BioDyne) on an ABI StepOnePlus™ Real-Time PCR System (Applied Biosystems). tubulin levels were measured and used as an internal control for RNA amount in each sample ...
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bioRxiv - Microbiology 2020Quote: One-step RT-PCR was realized with the SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase (Thermo Fisher Scientific) on an iCycler Thermal Cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR products were amplified from extracted RNA using a SuperScript™ III One-Step RT-PCR kit (Invitrogen, Carlsbad, CA, USA). A 20 μl reaction was assembled in PCR 8-tube strips through the addition of 10 μl 2× reaction mix ...
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bioRxiv - Microbiology 2020Quote: ... RT-PCR was performed using the Superscript III one-step RT-PCR system with platinum Taq DNA polymerase (Invitrogen, Carlsbad, CA, USA) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: ... was generated for each subject from 2,000 HIV-1 RNA copies by RT-PCR using SuperScript™ One-Step RT-PCR (Invitrogen, Carlsbad, California) followed by amplification using GoTaq colorless Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... real-time RT-PCR was performed using the SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Invitrogen) on the CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in an ABI PRISM 7500 Sequence Detection System (Applied Biosystems). Amplifications were carried out in 25 µL reaction mixtures containing 2× reaction mix buffer ...
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bioRxiv - Cell Biology 2019Quote: ... according to the manufacturer’s instructions and was used as the template for RT-PCR using Superscript IV One-Step RT-PCR System (Invitrogen, 12594-025). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... An 800 bp fragment covering the ORF10 region was amplified by one-step RT-PCR using the SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThermoFisher) and the following primers (OL11F ...
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bioRxiv - Microbiology 2023Quote: ... the extracted vRNA was converted to double-stranded cDNA and amplified using a one-step RT-PCR using SuperScript III One-Step RT-PCR kit (Thermo Fisher Scientific). The primers used were as follows ...
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bioRxiv - Neuroscience 2023Quote: Quantification of transcripts was performed by reverse transcription quantitative PCR (RT-qPCR) using the SYBR® Green RT-PCR assay (Applied Biosystems). Amplification ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Immunology 2020Quote: ... The expression of miR-155 and cel-miR-39 were analyzed by specific Taqman assays (ThermoFisher). Cel-miR-39 was used as an endogenous control to normalize expression ...