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Citations for Thermo Fisher :
451 - 500 of 9462 citations for 9 3 bromophenyl 9 phenyl 9H fluorene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: PC-9 and HCC827 cell lines were cultured at 37C and 5% CO2 in RPMI 1640 GlutaMAX media (Gibco, US) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... and loaded onto a 10% gelatin gel (9-10 µL for apical, 18-20 µL for basal, Novex, Life Technologies). Samples were electrophoresed for ∼90 min at 125-140V ...
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bioRxiv - Neuroscience 2022Quote: ... from each sample was labeled with 9 different TMT-10plex tags accordingly to the manufacturer’s protocols (cat# 90110, Thermo Scientific). The same mass tag and sample assignment was maintained throughout the entire study (old brain samples were labeled with TMT10-126 ...
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bioRxiv - Plant Biology 2022Quote: ... 9 RNA samples were extracted using TRIzol(tm) and RNA Purification Kit following the manufacturer’s protocol (ThermoFisher, Waltham, MA, USA). RNA was further purified using the TURBO DNA-free(tm ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... and samples were centrifuged at 8000xg for 5 minutes at −9°C in a Sorvall Legend Micro 17R (Thermo Scientific). The supernatant (extract ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was isolated from 9 µl lysate and 1 µl of ERCC 1:1000 Spike in control (Thermo Fisher Scientific) per sample using RNA SPRI beads (Agencourt ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Genetics 2020Quote: TaqMan copy number assays for CYP2D6 (assay IDs Hs04083572_cn and Hs00010001_cn for intron 2 and exon 9 respectively, Thermo Fisher Scientific) were run according to the manufacturer’s protocol on a ViiA7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 9 μL of total RNA samples were proceeded to cDNA synthesis using High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Then ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were cultured in DMEM with 10% FBS and 1% P/S and were bulked up to 9 x T175 flasks of each by splitting and reseeding during their growth phase (∼80% confluence) using TrypLE (Gibco). The 9 x T175 flasks were seeded at ∼30% confluence in serum-containing media ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviruses for expression of the DNMT1 were generated in Spodoptera frugiperda 9 (Sf9) cells according to the Bac-to-Bac system instruction (Invitrogen). The protein expression was performed by infection of the baculoviruses with the Sf9 cells for 72 hrs at 27 °C ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... day 9 spheroids were dissociated and stained for 30 minutes with Image-iT Lipid Peroxidation Sensor C11-BODIPY (Thermo Fisher) at a final concentration of 10 μM according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 20 µg of total RNA was reverse transcribed using 9 ng of oligo(dT)21 and 15 ng of random octamers (Invitrogen) in the presence of Cy3 or Cy5-dCTP (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Immunology 2021Quote: ... The 96-well plates w preloaded with 9 μL of RT-STA solution: 5 μL of CellsDirect PCR mix (Invitrogen), 0.2 μL of SuperScript-III RT/Platinum Taq mix (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Microbiology 2020Quote: ... CLR-1586) cells were cultured at 37°C in 9% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco™, ThemoFisher) supplemented with 5% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... PLAT-E cells were washed with 45 ml of PBS and were dissociated from the culture flask by a 5 minute incubation (37°C) with 9 ml of TrypLE reagent (Gibco), which was inactivated by adding 36 ml PBS ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures of Mtb were grown at 37 °C with shaking in Middlebrook 7H9 (Thermo Fisher Scientific, DF0713-17-9) broth supplemented with 0.02 % tyloxapol and albumin (Millipore Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection of and enrichment for cardiomyocytes was initiated on day 9 by culturing cells for 4 days in RPMI 1640 medium without glucose (Gibco) supplemented with 1x CDM3 supplement (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Libraries were amplified with 9 cycles of PCR reactions and quantified using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Libraries were sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Mouse glucagon-releasing cell line αTC1-9 (RRID: CVCL_0150, ATCC, Manassas, VA, USA) was cultured in DMEM (Invitrogen, Barcelona, Spain) without phenol red supplemented with 2 mM l-glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... obtained from a previous stock of SIP (9 parts of Percoll per 1 part of PBS 10X (GIBCO, 14200-067)) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of each of the three 9-LOX amplicons were mixed with 1μl of 6X gel loading dye (Thermo-Fisher Scientific), loaded on a 1% agarose gel and electrophoresed against a 1kb DNA ladder (RTU-Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... brucei 427 parasites were grown at 37°C and 5% CO2 in HMI-9 medium supplemented with Penicillin-Streptomycin (Gibco) and 10% Fetal Bovine Serum (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Established Schwann cells–DRG neuronal cocultures one week post dissection were infected with lentiviruses at an MOI ranging between 5 and 9 and incubated for 16 h in MEM (Invitrogen), 5% FBS (vol/vol ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was manually processed with 20 minutes heat-induced epitope retrieval at pH 9 followed by 15 minutes of proteinase K (1μg/ml; Life Technologies) digestion at 37°C ...
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bioRxiv - Cell Biology 2024Quote: Protein concentrations of samples (fractions 6-9) were determined with a Pierce™ BCA Protein Assay Kit (ThermoFisher, Rockford, USA), and Bradford assay (Biorad ...
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bioRxiv - Cell Biology 2023Quote: hSHEDs cultured at passage 9 were seeded in 100mm culture dishes at a density of 5000 per cm2 and grown in KnockOut DMEM (Gibco) media supplemented with 10% FBS (Gibco) ...
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bioRxiv - Bioengineering 2024Quote: ... AAV2/9 viruses were stored at −80°C until use and diluted with phosphate-buffered saline (PBS, Thermo Fisher Scientific) if necessary.
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Microbiology 2023Quote: ... after vaccination and boost (13 weeks after the beginning of the experiment or prime vaccination/ 9 weeks after Prime and Boost vaccination) were diluted in Opti-MEM media (Thermofisher) in serial 3-fold dilutions ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Biochemistry 2023Quote: ... inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30, Thermo scientific, Rockwood, TN). Samples were placed in an AI/AS 1300 autosampler prior to auto injection ...
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bioRxiv - Neuroscience 2023Quote: ... we conducted control experiments in WT mice by performing AAV2/9-hSyn-Flex-Synaptophysin-mRuby injection mixed with CTB-647 (C34778, ThermoFisher) into the IC ...
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bioRxiv - Cancer Biology 2023Quote: ... and the lower surface of the insert was stained with 0.5% crystal fast violet (548-62-9, Acros organics, USA). The cells were counted and captured under a Nikon Eclipse 80i microscope at 10× magnification.
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bioRxiv - Genomics 2023Quote: ... 9 ng of cDNA were used as template and reactions were performed using Power SYBR Green master mix (Applied Biosystems) on a StepOne Plus Real-Time PCR machine ...
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bioRxiv - Microbiology 2023Quote: ... 15 μl of 10% SDS (v/v) and 9 μl of PCR-grade proteinase K (20 mg/mL) (Thermo Fisher) for 30 min at 55°C ...