Labshake search
Citations for Peprotech :
101 - 150 of 185 citations for L Aspartic Acid N T Boc B Bz Ester 13C4 97 99%; 15N 97 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2019Quote: ... into B cell culture medium supplemented with 1 ng/mL recombinant mouse IL-4 (PeproTech Ca.# 214-14) and 2 μg/mL anti-mouse RP105 clone RP/14.
-
bioRxiv - Neuroscience 2020Quote: ... 200 μl day 14 medium additionally supplemented with 10 ng/ml CNTF (Peprotech, Cat N° 450-13B) was added to each of the four wells ...
-
bioRxiv - Immunology 2022Quote: ... in T cell medium supplemented with 2ng/ml TGFβ (R&D) and 100 U/ml rhIL-2 (PeproTech) in the presence of plate bound anti-CD3ε (1 μg/mL ...
-
bioRxiv - Genetics 2021Quote: ... T cells were transferred to the culture medium supplemented with 30 IU/mL IL-2 (Peprotech, 200-02A). After electroporation for 3 days ...
-
bioRxiv - Cell Biology 2024Quote: ... Isolated murine T cells were cultured in 10 ng / ml recombinant murine IL-7 (Peprotech, Cat # 217-17) in complete medium and used up to 5 days after isolation.
-
bioRxiv - Developmental Biology 2021Quote: ... NMP differentiation medium (-SBSAG/-SBLDN) or NMP differentiation medium supplemented with 50 ng/ml GDF11 (Peprotech, 120-11-B). Samples were collected for RNA analysis when confluent (48-72h).
-
bioRxiv - Bioengineering 2022Quote: ... 2.5 μg/mL amphotericin B and 10 ng/mL of human transforming growth factor-β3 (TGF-β) (Peprotech, UK).
-
bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
-
bioRxiv - Bioengineering 2022Quote: ... 2.5 μg/mL amphotericin B and 10 ng/mL of human transforming growth factor-β3 (TGF-β) (Peprotech, UK). After 2 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were activated by resuspending into R10 media containing 50 U/mL IL-2 (PeproTech) and 2 µg/mL anti-CD28 antibody (BD ...
-
bioRxiv - Immunology 2020Quote: ... Strips were washed with TBS-T (TBS, 0.1% Tween-20) and bound protein was detected with specific rabbit anti-chemokine antibodies (Peprotech) followed by an HRP-conjugated anti-rabbit antibody (Abcam ...
-
bioRxiv - Immunology 2023Quote: ... transduced T cells were rested for additional 2 days in fresh medium containing 10 ng/ml rhIL-2 (Peprotech) before 1×105 pMIG-empty vector (Ametrine+ ...
-
bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
-
bioRxiv - Cell Biology 2019Quote: ... 2 ng ml−1 recombinant human TGFβ1 (112 amino acid, HEK293-derived, Peprotech, 100-21). Cells were routinely maintained in E8 medium on 1:800 diluted growth factor reduced Matrigel (see below) ...
-
bioRxiv - Neuroscience 2021Quote: ... in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Sousa et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... In all experiments cells were cultivated for 72 h in culture medium as described above with addition of 10ng/mL human b-FGF (Peprotech) and culture medium was changed everyday ...
-
bioRxiv - Cell Biology 2020Quote: ... Adherent cells were then detached with a cell scrapper and seeded at a density of 5 × 104 cells/cm2 in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Bartell et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... or STEMDiff Astrocyte Maturation media without Supplement B with the addition of “TIC” proinflammatory factors (30ng/mL TNF-alpha (Cat No. 300-01A-10ug, Peprotech), 3ng/mL IL1-alpha (Cat No ...
-
bioRxiv - Bioengineering 2023Quote: ... After 2 days the coculture medium was replaced by coculture medium additionally containing 50 ng/mL receptor activator of NFκ-B ligand (RANKL, 310-01, PeproTech). Medium was changed weekly inside the incubator via the self-healing injection ports by removing 3 mL of the total 6 mL and replacing it by 3 mL fresh coculture medium with double the concentration of dexamethasone ...
-
bioRxiv - Biochemistry 2022Quote: ... For dot blot analysis of BMP-7 in conditioned media from co-culture assays 5 µl were applied to a nitrocellulose membrane and BMP-7 was detected using a rabbit pAb anti-BMP-7 GF at 1:2000 (#500-P198 B, Peprotech). For dot blot analysis in conditioned media of 3D organotypic co-cultures ...
-
bioRxiv - Neuroscience 2024Quote: ... media was changed to Neurobasal/GlutaMAX/B-27 with 20 ng/ml brain-derived neurotrophic factor (BDNF, Cat.# 450-02, Peprotech), 20 ng/ml glial cell line-derived neurotrophic factor (GDNF ...
-
bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
-
bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
-
bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
-
bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at a density of 1 million cells/mL in ImmunoCult-XF T cell Expansion Medium (STEMCELL) supplemented with 200 U/mL IL-2 (Peprotech) and stimulated with ImmunoCult Human CD3/CD28/CD2 T cell Activator (STEMCELL ...
-
bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
-
bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
-
bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% FBS and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega ...
-
bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
-
bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
-
bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
-
bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... the L-WRN condition medium was reduced to 5 % and supplemented with Noggin (Peprotech) and FBS.
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 50 ng/ml RANK-L and 30 ng/ml M-CSF (Peprotech) to stimulate osteoclastogenesis ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 292 ug/mL L-glutamine and 20 ng/mL human M-CSF (Peprotech) for at least 7 days ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 50 µM β-mercaptoethanol) supplemented with 50ng/mL hFIt3-L (PeproTech, 10773-618) and 2ng/mL GM-CSF (PeproTech ...
-
bioRxiv - Developmental Biology 2023Quote: ... AIC-N medium was composed of modified N2B27 medium supplemented with 10 ng/ml Activin-A (Peprotech, 120-14E), 2 μM IWP2 ...
-
bioRxiv - Immunology 2023Quote: ... After 2 days of stimulation and resting o/n in fresh medium containing 10 ng/ml rhIL-2 (Peprotech), T cell were labelled in modified RPMI 1640 containing 1 g/l 13C6-glucose (Sigma) ...
-
bioRxiv - Cell Biology 2021Quote: Immunofluorescent staining was in general performed on HUVECS cultured on 12 mm glass coverslips coated with 5 μg/ml FN and treated with or without o/n TNFα (10ng/ml) (Peprotech), washed with PBS+/+(1mM CaCl2 and 0.5mM MgCl2) ...
-
bioRxiv - Molecular Biology 2020Quote: ... IFN-γ (500 U/L) and IL-17 (10 ng/ml) were obtained from PeproTech. For short-term stimulation ...
-
bioRxiv - Immunology 2022Quote: ... 1 % L-Glutamin in RPMI) supplemented with 100 units/mL IL-2 (Peprotech, 200-02) for the respective amount of time ...
-
bioRxiv - Immunology 2019Quote: ... and 100 μm non-essential amino acids) with 100 U/ml of recombinant human IL-2 (PeproTech), which was replaced every 2 days keeping the cells at a concentration of 1.5 × 106 cells/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... devoid of fatty acids and supplemented with the growth factors GDNF (20 ng/mL; Peprotech, NJ, USA) and FGF2 (1 ng/mL ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
-
bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
-
bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...