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Citations for Peprotech :
401 - 450 of 1510 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
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bioRxiv - Neuroscience 2023Quote: ... and plated onto 3 wells of a 6-well plate precoated with GFR Matrigel in N2B27 with 100ng/ml FGFb (100-18B, Peprotech) and 100ng/ml EGF (AF-100-15 ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Cell Biology 2023Quote: ... glutamine and human early-acting cytokines (SCF 300 ng/ml, Flt3-L 300 ng/ml, TPO 100 ng/ml, and IL-3 60 ng/ml; all purchased from Peprotech). All cultures were kept at 37°C in a 5% CO2 water jacket incubator (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were cultured with stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (FLT3L) (Peprotech, Rocky Hill, USA) for the first 4 days ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra-low attachment 6-well plates (Costar ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 μl of N2B27 medium supplemented with 100 ng/ml Activin A (QKine, Cambridge, UK) and 3 μM chiron (Peprotech) was added to each well ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow was stimulated with recombinant murine IL-3 (10 ng/mL) and IL-6 (10 ng/mL) (Peprotech, 21616) for 48 hours ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 days and then cultured in 10% FBS α-MEM media supplemented with 100ng/mL RANKL (PeproTech, 315-11) and 30 ng/mL M-CSF for 6 to 7 days ...
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bioRxiv - Immunology 2024Quote: ... The isolated pDCs (85% purity) were maintained overnight in the presence of IL-3 (1 ng/ml) (PeproTech, London, UK) before stimulation.
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bioRxiv - Immunology 2024Quote: ... splenocytes were adjusted to a concentration of 3 x 106 cells/mL in T cell medium containing 10 ng/mL rhIL-7 (Peprotech) and 2 µg/mL anti-mouse CD28 (clone 37.51 ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 ng/ml FLT3-L, 20 ng/ml IL-3 and 20 ng/ml G-CSF; all purchased from Peprotech) supplemented with 1% penicillin–streptomycin (ThermoFisher Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 μM CHIR treatment was performed from day 4 alongside 50ng/ml Fgf2 (Murine FGF-basic, Peprotech, 450-33-100). CHIR treatment was maintained for two days while Fgf2 was maintained for three additional days.
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bioRxiv - Immunology 2021Quote: ... 10mM non-essential amino acids and 10ng/mL IL-6 (Peprotech)] ...
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bioRxiv - Systems Biology 2023Quote: ... and subsequently cultured in complete culture media supplemented with 5 nM human IL-2 (Peprotech) for up to 10 days ...
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bioRxiv - Immunology 2023Quote: ... supplied with 5 % human AB serum (MP Biomedical) and 10ng/mL human IL-2 (Peprotech), hereafter referred to as cX-VIVO ...
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bioRxiv - Immunology 2022Quote: ... We further supplemented the media with 5 ng/mL recombinant human (rh) IL-2 (Peprotech) and 10 ng/mL rhIL-7 ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Gemini) and 30 IU ml-1 recombinant human IL-2 (Peprotech). Primary human CD8+ T cells were left unstimulated or were stimulated with anti-CD3/CD28 dynabeads (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... cells were starved for 3 h by removal of serum from the medium and then stimulated in duplicate with TNF-α (40 ng/ml, PeproTech) in FBS-free DMEM for 0 ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Cell Biology 2023Quote: ... On day 3, medium was replaced with E6 supplemented with 50 ng/mL vascular endothelial growth factor (VEGF, (PeproTech, 100-20), 10 ng/mL FGF2 and 20 ng/mL rBMP4 ...
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bioRxiv - Immunology 2024Quote: ... and 1% non-essential amino acids) containing 5ng/mL IL-15 (Peprotech) to activate NK cells ...
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bioRxiv - Immunology 2021Quote: ... and were stimulated with mIL-2 (200 U/mL) and hTGF-β1 (5 ng/mL, PeproTech) for iTreg differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... from serial transplants were thawed and maintained in medium (DMEM with 15% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13), 10ng/ml IL-6 (Peprotech 216-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2020Quote: ... were maintained in medium (RPMI with 20% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13). For in vivo experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2024Quote: ... bone marrow precursor cells were obtained from flushed femurs and tibias of C57BL/6J mice and cultured at 3*106 cells/ 10 ml in petri dishes in fully supplemented RPMI and rmGM-CSF (200 U/ml; Peprotech, Hamburg, Germany). Medium was exchanged on days 3 and 6 and the cells were cryopreserved on day 7 for further use.56
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bioRxiv - Neuroscience 2024Quote: ... EBs cell culture media was replaced with X-VIVO 15 media supplemented with 50 ng/ml Flt-3-ligand (PeproTech, 300-19), 50 ng/ml M-CSF (PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Developmental Biology 2024Quote: ... the medium was changed to late hCNCCs medium by supplementing the early hCNCCs medium with 3 µM CHIR 99021 (Selleckchem, catalog no. S2924) and 50 pg/mL BMP2 (Peprotech, catalog no. AF-120-02) to enhance cell proliferation and reduce migration28 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were detached using TrypLE and seeded on nongelatinized bacterial dishes for suspension culture at 3 million cells per 75 cm2 and cultured in N2B27 containing 10 ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days ...
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bioRxiv - Neuroscience 2022Quote: ... coated 6 well plates at 200,000 cells/well in 2 ml/well StemFlex supplemented with ROCK inhibitor Y-27632 (5 mM, PeproTech) to inhibit apoptosis ...
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...