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Citations for Peprotech :
2051 - 2100 of 2832 citations for IL 1 beta Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... glutamine and human early-acting cytokines (SCF 300 ng/ml, Flt3-L 300 ng/ml, TPO 100 ng/ml, and IL-3 60 ng/ml; all purchased from Peprotech). All cultures were kept at 37°C in a 5% CO2 water jacket incubator (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... washed one time with PBS and re-plated in a newly irradiated 40LB feeder layer in the presence of 10 ng/ml IL-21 (PeproTech) for the next 4 days ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
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bioRxiv - Immunology 2023Quote: ... CD39+ γδ IELs were treated with 100 μM POM-1 (Biotechne) for 2 h prior to the addition of 10 μM ATP (ThermoScientific) and IL-2 (6 ng/ml, Peprotech). Supernatants were collected and stored at -20°C for future use ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... and light zone cells (CD19+GL7+FAS+CXCR4-CD86+) and cultured in the presence of 200 ng/ml Il-9 (PeproTech) in RPMI media for 3 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of 500,000 cells were plated in 200 µL of media in a 96-well plate and stimulated with 20 ng/mL of IL-6 (Peprotech) for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... CLL PBMCs were cocultured on irradiated NIH3T3 fibroblasts stably transfected with CD40 ligand or parental cells with or without interleukin-4 (IL-4, 10ng/ml, Peprotech) and IL-21 (25ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... CD8 T cells were transferred to a new 96-well round bottom plate to remove activation and continued to be cultured in complete RPMI supplemented with a higher concentration of IL-2 (10ng/ml; Peprotech).
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bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: Naïve and activated CD4+ T cells (via 72h incubation with αCD3/28 beads) were either left untreated or stimulated with IL-10 (40 μg/ml, PeproTech) for 20 min and then lysed with RIPA buffer containing proteases and phosphatases inhibitors (MilliporeSigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was collected from wells pre-stimulated with 20 ng/ml TNFα and 20 ng/ml IL-1β (both from PeproTech) for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: IL-12 accessibility to monoclonal antibody binding was determined by using the antibodies in an IL-12 sandwich ELISA kit (Peprotech). After coating a 96 well plate with anti-IL-12 antibodies and blocking the plate with bovine serum albumin according to the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech). After 5 days ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech) and 4% CHO-SCF conditioned media ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...
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bioRxiv - Immunology 2024Quote: ... Samples were stimulated and incubated overnight in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech).
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bioRxiv - Biophysics 2021Quote: ... bone marrow progenitor cells were cultured in complete RPMI media supplemented with interleukin-4 (IL-4, 10 ng/mL, PeproTech, Canada) and granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...
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bioRxiv - Immunology 2021Quote: ... as indicated (IFN-γ, 10ng/mL, 315-05; TNF-a 20ng/mL, 315-01; IL-17A 10ng/mL, 210-17; all from PeproTech). Eosinophils were washed with PBS and transferred to a solid white flat bottom 96-well plate (Corning ...
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bioRxiv - Immunology 2019Quote: ... and maintained in HIPP™-T009 medium (Bioengine) in the presence of 2% autoserum and IL-21 (30 ng/ml, PeproTech) in a 37 °C 5% CO2 incubator for 12 days ...
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bioRxiv - Bioengineering 2020Quote: ... A Th2 microenvironment was induced by perfusing the vascular channel of the Airway Lung-Chips with 100 ng/mL of IL-13 (Peprotech, USA) for 7 days ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Immunology 2020Quote: BMDMs were stimulated with the following cytokines where indicated unless otherwise noted: 20 ng/mL of IL-6 (Peprotech, 212-16), 10 ng/mL of IL-18 (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Immunology 2020Quote: PBMCs were plated in 24-well plates at 2×106 cells per well with 100 IU/ml IL-2 (PeproTech, USA) in the complete medium ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Immunology 2023Quote: ... ROSAKIT WT cells were seeded at 0.3 x 106 cells/ml and treated for 4 days with IL-4 (20 ng/ml, PeproTech, #200-04) and human IgE (1 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... in the absence or presence of 10 ng/mL IL-1β (FujiFilm Wako Pure Chemical) and 20 ng/mL TNF-α (PeproTech). Differentiated astrocytes expressing ERT2-E2F1-1′C were prepared from NPCs that were previously infected with retrovirus encoding ERT2-E2F1-1′C followed by selection with 0.25 μg/mL puromycin (Nacalai tesque) ...
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bioRxiv - Neuroscience 2021Quote: ... or in combination with 25 ng of human recombinant BDNF (Peprotech, 450-02) or 25 ng of human recombinant GDNF (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... and 10ng/mL recombinant human macrophage colony-stimulating factor (Peprotech, East Windsor NJ) for 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... 20 ng/mL recombinant human epidermal growth factor (EGF, PeproTech, Rocky Hill, NJ) and 20 ng/mL recombinant human fibroblast growth factor-2 (FGF-2 ...
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bioRxiv - Bioengineering 2019Quote: ... or TexMACS (Miltentyi Biotech) supplemented with 400 U/mL recombinant human IL2 (Peprotech). Cell cultures were expanded for 14 d as counted from the time of initial seeding and activation ...
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bioRxiv - Cancer Biology 2020Quote: ... containing human epidermal growth factor (hEGF, 20 ng/ml, Peprotech; AF-100-15), human fibroblast growth factor 2 (hFGF2 ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human Fibroblast Growth Factor 9 (FGF9, Peprotech, 100-23), 500 ng/mL Prostaglandin D2 (PGD2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 2.5 ng/ml human fibroblast growth factor-basic (hFGF2) (PeproTech, Germany #100-18B), 50 mg/ml penicillin ...
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bioRxiv - Cancer Biology 2021Quote: ... The endothelial monolayer was then incubated with human TNFα (10 ng/ml; PeproTech) for 16h ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human TGFβ and PDGF were obtained from Peprotech (Rocky Hill, CT, USA) and used at a final concentration of 5 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: ... and recombinant human FGF-basic at a concentration of 10 ng/mL (Peprotech). hADSCs were passaged upon reaching ~80 % confluency ...