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Citations for Peprotech :
2301 - 2350 of 2832 citations for IL 1 beta Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: Small molecules and cytokines were supplemented as indicated in screening related figure legends: recombinant human BMP4 (10ng/ml, Peprotech), BMPRi (LDN193189 0.3µM ...
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bioRxiv - Developmental Biology 2020Quote: ... or C57BL6/129sJae EpiSC line (derived following in vitro priming of V6.5 mouse ESCs) were expanded in N2B27 with 12ng/ml recombinant human bFGF (Peprotech) and 20ng/ml recombinant Human ACTIVIN A (Peprotech) ...
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bioRxiv - Genomics 2020Quote: ... cells were assessed with and without 16h pretreatment of 10 ng/ml recombinant human IFNγ (Peprotech, Rocky Hill, NJ).
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bioRxiv - Cell Biology 2019Quote: ... This medium was further supplemented with vitamins and minerals (Table 4), thyroxine (Whitney et al., 2018) and recombinant human TGFβ1 (Peprotech). Medium sampling ...
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bioRxiv - Biochemistry 2022Quote: ... lung organoids were cultured in medium including recombinant human transforming growth factor (TGF)-β1 (Peprotech, Rocky Hill, NJ, USA) without A83-01 (Tocris ...
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bioRxiv - Bioengineering 2022Quote: ... 2.5 μg/mL amphotericin B and 10 ng/mL of human transforming growth factor-β3 (TGF-β) (Peprotech, UK).
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bioRxiv - Biochemistry 2021Quote: ... they were cultured in the presence of 0.5% FBS for the last 24 h and then incubated with 100-ng/ml human EGF (PeproTech) at 37°C for 5 min.
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bioRxiv - Neuroscience 2021Quote: ... organoids were also generated as described excepting the addition of 25ng/mL recombinant human PTN (Peprotech, CAT#: 450-15) into differentiation media for 7d.
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bioRxiv - Immunology 2020Quote: ... Cells were polarized into M1 macrophages by 20 h incubation with human interferon (IFN)-γ (PeproTech, 20 ng/mL) and ultrapure LPS (10 pg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... 2.5 μg/mL amphotericin B and 10 ng/mL of human transforming growth factor-β3 (TGF-β) (Peprotech, UK). After 2 days ...
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bioRxiv - Immunology 2023Quote: ... at a density of 7.5×105 cells/ml in 2 ml complete media supplemented with 20 ng/ml recombinant human GM-CSF (Peprotech). The plates were incubated at 37°C in 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium) ...
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bioRxiv - Bioengineering 2024Quote: ... naive primary human CD4+ T cells were cultured on-chip for 24 h with media spiked with 1.22 ng/mL of recombinant human CCL21 (Peprotech). Brightfield images were acquired using transmitted light in time series mode (1 image was collected every 30 sec for 10 cycles ...
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bioRxiv - Immunology 2023Quote: ... tumor suspension and PBMCs were thawed and plated with recombinant human IFNy at 200 ng/mL (Peprotech, #300-02) (only for tumor suspension ...
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bioRxiv - Immunology 2022Quote: ... we thawed cryopreserved TCRα/β-depleted BM fractions at 37°C and cultured them overnight in SFEM containing 100 ng/mL human stem cell factor (SCF, Peprotech), 100 ng/mL human Fms-related tyrosine kinase 3 (FLT3 ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ... 25ng/ml human FL and 10ng/ml murine IL3 with or without 25ng/ml murine M-CSF (all from PeproTech). No apparent difference was observed between cultures with or without M-CSF and data was combined ...
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bioRxiv - Biochemistry 2019Quote: ... CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF) and recombinant human interferon gamma (50 ng/ml IFNγ) (Peprotech) in standard tissue culture DMEM media containing fetal calf serum ...
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bioRxiv - Developmental Biology 2020Quote: ... Resuspended cells were filtered with 35 µm filter (Falcon) and sorted into sorting buffer (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... and vascular endothelial growth factor (VEGF) (Catalog No. 100-20, Recombinant Human VEGF165, Peprotech, Rocky Hill, NJ, 0.1 μg/mL). A mixture of heparin ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells at a plate confluence of 80% were treated for 10 min with 100 ng/mL animal-free recombinant human EGF (PeproTech) or Gibco™ distilled water (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... In all experiments cells were cultivated for 72 h in culture medium as described above with addition of 10ng/mL human b-FGF (Peprotech) and culture medium was changed everyday ...
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bioRxiv - Bioengineering 2022Quote: ... All the supernatants were evaluated by using a human FGF-basic standard ABTS enzyme-linked immunosorbent assay (ELISA) development kit and ABTS ELISA buffer kit (both Peprotech).
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bioRxiv - Systems Biology 2020Quote: ... Cells were grown to at least 500,000 cells/mL before treatment with 20 ng/mL recombinant human tumor necrosis factor α (TNF) (Peprotech), 300 nM A-485 (Structural Genomics Consortium) ...
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bioRxiv - Microbiology 2020Quote: ... On DIV 10 add 100% N2 medium plus recombinant human Brain Derived Neurotrophic Factor 10ng/ml (BDNF 450-02; Peprotech); Glial-Derived Neurotrophic Factor 10ng/ml (GDNF 450-10 ...
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bioRxiv - Cell Biology 2021Quote: Primary mouse AT2 cells were isolated from homozygous H991A adult (mice 6-8 weeks) and cultured in BEGM plus 10% charcoal-stripped FBS and recombinant human KGF (10 ng/ml, Peprotech) on top of a thin substrate of 70% Cultrex (Trevigen)/30% rat tail collagen (Advanced Biomatrix ...
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bioRxiv - Cell Biology 2020Quote: ... After 2 days of culture with 20 ng/ml M-CSF and 20 ng/ml recombinant human RANKL (PeproTech, UK), the wells were rinsed twice with PBS and incubated with 10% bleach solution for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... lines were maintained in StemFiT AK02 media (Ajinomoto) supplemented with 100 ng/ml recombinant human basic fibroblast growth factor (bFGF, Peprotech) (hereafter F/A media ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... emerging hematopoietic cells and EBs were cultivated in SFM ((Liu et al. 2015) supplemented with recombinant human 10 ng fiblroblast growth factor type 2 (rhFGF-2, Peprotech), recombinant human 50 ng/mL rhSCF and 30 ng/mL rhIL-3 ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum and M-CSF (10 ng/ml, Peprotech) for 3 days and then differentiated for a further 4 days in RPMI 1640 medium supplemented with 10% fetal calf sera without M-CSF.
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 100 ng/ml recombinant human FLT3-L (Peprotech). After three days of differentiation ...
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bioRxiv - Developmental Biology 2022Quote: ... #D1-011) according to manufacturer instructions by supplementing with 200 ng/ml human recombinant Sonic Hedgehog (Peprotech, Cat. #100-45) from day 0-10 and passaging cells at day 0 ...
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bioRxiv - Bioengineering 2022Quote: ... the spheroids were then transferred to spinal cord medium II (ScM II) with 10 nM retinoic acid and 5 ng/mL recombinant human BMP4 (Peprotech) for the next 6 days ...
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bioRxiv - Developmental Biology 2022Quote: The medium was further supplemented with growth factors and signalling modulators comprising of human Epidermal Growth Factor (hEGF) (12ng/ml, PeproTech), B27 Supplement (2% ...
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bioRxiv - Developmental Biology 2022Quote: ... These epithelial cells were then propagated in a complete medium supplemented with the addition of human Epidermal Growth Factor (hEGF) (12ng/ml, PeproTech), ROCK inhibitor (Y-27632 ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in RPMI 1640 containing 20% FBS and 50 ng/ml human macrophage colony-stimulating factor (M-CSF) (300-25, Peprotech). Cells were seeded into sterile tissue-culture plates and differentiated for 7 days ...
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bioRxiv - Neuroscience 2023Quote: ... iSNs were treated with 10% plasma or 100ng/mL recombinant human ET1 (R&D, #1160) or CCL2 (PeproTech, #300-04) diluted in iSN maturation media for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2022Quote: Leptin concentration within all collected samples was determined using the Human Leptin Standard ABTS ELISA Development Kit (PeproTech #900-K90) and ABTS ELISA Buffer Kit (PeproTech #900-K00 ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... expanded neural progenitors were re-plated at a density of 3×104 cells/cm2 in expansion media and 24 hours later switched to N2B27 supplemented with 10 ng/mL human recombinant CNTF (Peprotech) and 10 ng/mL human recombinant BMP4 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...
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bioRxiv - Genomics 2024Quote: ... macrophages were cultured in macrophage media supplemented with 20 ng/ml human recombinant IFN-γ (PeproTech, catalog no. 300-02) and 25 ng/ml LPS (Invivogen ...
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bioRxiv - Immunology 2023Quote: Primary Keratinocytes from three donors were seeded in 24-well plates and stimulated with KGM Media supplemented either with 0.1 ng/ml or 10 ng/ml of recombinant human IFN-γ (PeproTech), or infected with T ...
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bioRxiv - Bioengineering 2023Quote: ... At Day 11 the cytokine composition of the media was changed to 10ng/μl of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/μl (Peprotech). From Day 15 onwards ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were assayed immediately or cultured overnight in StemSpan SFEM (STEMCELL) with 50 ng/mL recombinant human stem cell factor (PeproTech), 10-6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... On day 11 the cytokine composition of the media was changed to 10ng/ml of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/ml (Peprotech). From day 15 onwards ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Cell Biology 2020Quote: ... with 500 nM SB431542 (Selleckchem, cat. no. S1067) and 20 ng/ml recombinant human (rh)Noggin (Peprotech, cat. no. 120-10C). On days 5-9 ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with 25 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 24 hours.
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bioRxiv - Cancer Biology 2019Quote: ... 20ng/mL human recombinant epidermal growth factor (EGF) and 20ng/mL basic broblastic growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ).