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Citations for Peprotech :
1651 - 1700 of 1705 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions of cell lines were suspended at a density of 2,000 cells/ml in DMEM/Ham’s F-12 (nacalai tesque, no. 11581-15) containing 20 ng/ml bFGF (Peprotech, no. 100-18B), 20 ng/ml EGF (Wako ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... 33010-018)-coated plates at a density of 30 000 cells/cm2 and by supplementing the N2B27 medium with 12 ng/ml of bFGF (Peprotech, 100-18B) and 20 ng/ml of activin A (Peprotech ...
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bioRxiv - Immunology 2019Quote: ... Samples were adjusted to 7.5×105 cells/mL with cold cRPMI_M supplemented with 25 ng/mL of recombinant mouse colony-stimulating factor (m-csf, Peprotech, Rocky Hill, NJ) and cultured at 37°C ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2021Quote: ... Purified microglia cells were span down at 300 × g and plated at 100 103 cells per well in microglia media supplemented with 100 ng/ml of rhIL34 (Peprotech, 200-34), 2 ng/ml TGFb2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Developmental Biology 2021Quote: ... Differences were that for mouse cells the medium for aNSCs was additionally supplemented with 20 ng/ml recombinant murine Epidermal Growth Factor (PeproTech 315-09) and qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Immunology 2020Quote: ... and PD-L1 were performed as follows: 2e8 cells of the knockout (KO) library was stimulated with IFNγ (10ng/mL; Peprotech 315-05) for 24 hours after which cells were harvested by scraping to ensure integrity of cell surface proteins ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Genomics 2023Quote: ... with cells in the third or subsequent passages defined as ‘late hCNCC’ and cultured with added 50 pg/ml BMP2 (Peprotech, 120-02) and 3 uM CHIR 99021 (Selleck ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2023Quote: Jurkat and THP1 WT and ARPC5 KO cells were seeded in 96-well plate and stimulated with RPMI 1640 + 100 ng/ml CXCL12 (Peprotech, 300-28A) or RPMI 1640 for the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Biophysics 2021Quote: ... EMT in all cells lines were induced by addition of 5ng/ml Targeted Growth Factor-β1 (TGFβ1) (Peprotech, catalog no. 100-21-10UG) for 48 hours62.
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Immunology 2019Quote: ... the suspension cells were removed and culture in a new flask along with 10ng/ml recombinant murine IL-3 (Peprotech, Rocky Hill, NJ). Cells were passaged twice per week ...
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bioRxiv - Cancer Biology 2021Quote: ... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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bioRxiv - Immunology 2020Quote: ... Mouse T cells with the addition of mercaptoethanol and patients’ T cells were incubated in the presence of 10ng/mL of hrIL2 (PeproTech, Rocky Hill, NJ). The identity of all cell lines was evaluated using short tandem repeat profiling performed in-house at the Genomic Core Facility ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2022Quote: ... and histone isolation procedures activated CD8+ Teff cells were re-cultured (5 × 106 cells/well in 6-well plates) for up to 48h in IMDM containing 50 U/mL IL-2 (PeproTech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Immunology 2022Quote: ... The following cytokines were added at the indicated concentrations for murine γδT cells: recombinant murine IL-12 (10ng/ml) (Peprotech, Rocky Hill, NJ), IL-15 (50ng/ml ...
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bioRxiv - Immunology 2022Quote: ... bone marrow was isolated from tibiae and femurs and single-cell suspensions were cultured at 10*106 cells per 100 mm bacteriological Falcon petri dish in 10 ml complete T cell medium supplemented with 20 ng/ml GM-CSF (PeproTech, Rocky Hill, NJ). At day 3 ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 cells/ml were seeded in a 96 well plate and treated with IFN-γ (20ng/ml, Cat#315-05, Peprotech, NJ, USA), IL-21 (20 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... For cytokine treatment cells were stimulated with either 5 ng/mL of TGFβ1 or 50 ng/mL of BMP6 (both from PeproTech, Rocky Hill, NJ, US). Where indicated ...
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bioRxiv - Microbiology 2023Quote: ... for 48h at a concentration of 1 bead/cell in the presence of 100 U/ml IL-2 (PeproTech, Inc., Cat. #200-02) in RPMI +/+ and were subsequently cultured under hypoxia (HOX - 1% O2 ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were allowed to recover at a density of 1E6 cells/mL in antibiotic-free medium containing 100 IU/mL IL-2 (PeproTech, Rocky Hill, NJ) for 72 hours at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Matrices were placed into ImmunoCult™-XF T Cell Expansion Medium (StemCell, Cat: 10981) supplemented with rhIL-2 (200IU; Peprotech, Cat: 200-02), rhIL-7 (10ng/mL ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Cancer Biology 2021Quote: Primary mouse or NMR fibroblasts were seeded at 1 × 104 cells/well onto 24-well plates and stimulated with TNF-α (PeproTech; 50 ng/mL), z-VAD-fmk (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... a small number of spheroids were triturated into a single cell suspension and plated on Matrigel coated glass cover-slips at ~10k cells/cm2 using ASM plus CNTF and BMP4 (Peprotech, 450-13 & 120-05ET). Media was changed every 48 hours ...
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bioRxiv - Neuroscience 2023Quote: ... was added at a final concentration of 3.4 mg/mL to prevent the growth of non-neuronal cells and supplemented with 50 ng/mL murine β-nerve growth factor (PeproTech, Cat# 450-34) with 3 mg/mL DNAse I grade II (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...