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Citations for Peprotech :
1551 - 1600 of 1705 citations for Primary Human Aortic Smooth Muscle Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... CML CD34+ cells were cultured at 100,000 cells/ml in SFM medium with the addition of EPO (3 U/ml, catalogue no. 100-64; PeproTech). Following a 72h incubation ...
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bioRxiv - Immunology 2023Quote: ... The cells were stimulated with IL-12+IL-18 (10 ng/ml, Peprotech, and 50 ng/ml, MBL, respectively) or plate-bound anti-CD16 (1 mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The unattached cells were collected and plated in 96-well plates with MCSF (50ng/ml, PeproTech, Rocky hill, USA)18added ...
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bioRxiv - Cell Biology 2023Quote: ... were passaged and replated at a density of 100,000 cells cm-2 then treated with 10 ng/ml FGF2 (100-18B, PeproTech) and 10 μM SB431542 (S1067 ...
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bioRxiv - Molecular Biology 2024Quote: K562 cells were cultured in 12-well plates and treated with 10 ng/mL interferon beta (PeproTech, #300-02BC) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
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bioRxiv - Immunology 2024Quote: ... stem cell factor (SCF; 5% supernatant of murine SCF-secreting CHO transfectants) and IL-4 (1 ng/ml, PeproTech) were added to the medium (89) ...
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bioRxiv - Immunology 2024Quote: ... Edited cells were cultured and expanded in R10 containing 5ng/ml IL7 and 5ng/ml IL15 (Peprotech #20007, #20015) and replenished every 2-3 days until freezing at days 14-16.
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were re-suspended in DMEM/F12 complete medium containing 20 ng/mL recombinant murine FGF (PeproTech, 450-33), 20 ng/mL recombinant murine EGF (PeproTech ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were maintained in complete RPMI (10% FBS, 1% Pen/strep) with 30 U/mL rIL-2 (Peprotech, 200-02). To infect ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were then plated at 106 cells/ml in cRPMI containing 20ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF, Peprotech), and cultured for 7 days ...
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bioRxiv - Molecular Biology 2019Quote: ... MLO-Y4 cells were treated with the following reagents in serum-free media: 5 ng/ml TGFβ1 (Peprotech, 100-36E), 0.1 μM PGE2 (Sigma) ...
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bioRxiv - Systems Biology 2019Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL SCF (Peprotech), 5 ng/mL Flt3-L ...
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bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
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bioRxiv - Cancer Biology 2019Quote: ... At day 12 cell cultures were enriched with hNCCs and cultured in N2B27 supplemented with bFGF and EGF (both 20ng/ml, Peprotech) and 3 μM CHIR99021.
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bioRxiv - Neuroscience 2020Quote: ... in the presence of freshly added 20 ng/ml mouse glial cell line-derived neurotrophic factor (GDNF, PeproTech, 450-44) as detailed elsewhere (Sleigh et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: After 48 hours of culture in preculture medium (complete IMDM medium supplemented with 10% FCS, stem cell factor (SCF) (100 ng/mL, Peprotech), FMS-like tyrosine kinase-3 ligand (FLT3-L ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Neuroscience 2020Quote: ... Neuronal maturation and terminal differentiation were achieved by plating the neural stem cells in the density of 25,000-35,000 cells/cm2 in the Neural Maturation Media (NMM) composed of NBM supplemented with BDNF (20ng/ml) (450-02, Peprotech), GDNF (10ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... At day 8 cells were detached and aggregated in the U bottom microwells in the presence of 20 ng/mL HGF (PeproTech), 10 ng/mL oncostatin M (OSM ...
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bioRxiv - Immunology 2019Quote: ... One week later cells were harvested and seeded on well plates and incubated for one day with GM-CSF (20 ng/mL; Peprotech). Training experiments were performed with these cells according to the Monocyte training protocol ...
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bioRxiv - Immunology 2022Quote: ... the cells were supplemented with murine rIFN-γ (40 μg/ml) (Recombinant Murine IFN-γ, Catalog Number:315-05, Peprotech) or were kept unaltered ...
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bioRxiv - Immunology 2022Quote: ... The cells were cultured in the presence of 20 ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF; from Peprotech) in complete DC medium (CDCM) ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Neuroscience 2019Quote: ... to a density of 220 000 cells/cm2 in N2B27 Medium containing 10 μM Rock inhibitor and 20 ng/ml FGF2 (Peprotech). The medium was changed every third day without Rock inhibitor ...
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bioRxiv - Immunology 2021Quote: ... Sorted HSC from wt or CD150-/- mice were cultured in StemSpan SFEMII (Stem Cells) complemented with 50 ng/μL TPO (Peprotech) and 20 ng/μL SCF (Peprotech) ...
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bioRxiv - Genetics 2019Quote: ... monocytes were plated in a 100mm x 20mm dish and cells were treated with 800 U/ml M-CSF (PeproTech) for seven days ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
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bioRxiv - Immunology 2021Quote: ... Optimized TCR constructs were retrovirally transduced in CD8+ healthy donor derived T cells as described before and expanded for 7 to 10 days with IL-7 and IL-15 (5ng/mL; Peprotech) before functional characterization (13,14).
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bioRxiv - Molecular Biology 2021Quote: ... in 96-well plates (5.0 x 105 cells/well) and incubated with DMEM containing 10% FCS in the presence of 100 ng/ml recombinant murine RANKL (PeproTech) with 20 ng/ml recombinant murine M-CSF (PeproTech) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were dissociated and 3×106 cells were plated in a bacterial petri dish in N2B27 medium supplemented with EGF 10 ng/mL (Peprotech) and FGF2 10 ng/mL (R&D systems) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Immunology 2022Quote: ... primary neuronal cells were stimulated with 30 ng/μl or 100 ng/μl of Recombinant Murine IL-12p70 (#210-12, Peprotech) for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 x 105 cells were seeded on collagen-coated 60 mm dishes and treated daily with 200 pM TGF-β1 (100-21; PeproTech) dissolved in DMEM media with 1% BSA and 1% penicillin/streptomycin for 4 days ...
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bioRxiv - Bioengineering 2022Quote: ... and then for three to five days (plasmablast/plasma cell differentiation) in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2022Quote: ... Quiescence was induced by plating cells at a density of 10 cells/mm2 in NSC media in the absence of EGF/FGF-2 and supplemented with BMP4 (10 ng/ml, Peprotech). Cells were treated for 1 day or 3 days ...
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... TCR condition – cells activated with anti-CD3/CD28 antibodies on day 0 and cultured with IL-2 (2 ng/ml, PeproTech); TCR+TCR condition – cells activated with anti-CD3/CD28 on day 0 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured in RPMI 1640 medium with 10% FBS containing GM-CSF (25 ng/ml; Peprotech, Rocky Hill, NJ) and IL-4 (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The iPSC colonies were maintained in undifferentiated pluripotent state by culturing the cells under feeder free conditions on 0.1% Geltrex® (Peprotech) coated culture plates ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Molecular Biology 2022Quote: ... Both SFEM II and our custom expansion media were supplemented with 100 ng/mL stem cell factor (Peprotech, 250-03), 40 ng/mL insulin-like growth factor (Peprotech ...