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Citations for Peprotech :
1401 - 1450 of 1458 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... HFFs treated with IFN were incubated with 550U/ml IFNα (PBL; 11200-2) and 700U/mL IFNβ (Peprotech; 300-02BC-20) and harvested after 5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Molecular Biology 2024Quote: ... EBs were dissociated into single cells and re-aggregated as MES cells for 40 h at 50,000 cells/ml in the presence of 5 ng/mL human VEGF (Novoprotein, C083) and 10 ng/ml human Activin A (Peprotech, 96-120-14-10) and 0.3 ng/ml human BMP4 (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... Catalog No.501362646), 10 μM SB (SB431542, Selleckchem, Catalog No.101762-616) and 5 ng/mL FGF2 (FGF basic 154aa, human, Peprotech, Catalog No.10771-938).
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bioRxiv - Developmental Biology 2020Quote: ... the culture medium was replaced with fresh DMEM/F12 with 2% FBS supplemented with TGFβ1/TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5mM citric acid (diluent for lyophilized TGFβ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2020Quote: ... 50 uL HS (250 mM NaCl) and PS (145 mM NaCl) gels (10 mg/mL fibrinogen) were fabricated with 100 ng/mL FGF-2 (Peprotech, 100-18b) and 100 ng/mL VEGF-165 (Shenandoah Biotechnology ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 6 hours in fresh media lacking cytokines and subsequently treated with 100 U/mL of either IL-2 or IL-15 (PeproTech 200-15).
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and further cultured for two days in S1/2 medium supplemented with FGF7 (50ng/ml) (PeproTech, 100-19) and vitamin C (0.25mM ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... DE cells were rinsed with PBS and then exposed to S1/2 medium supplemented with 50 ng/ml KGF (FGF7) (PeproTech, 100-19) and 0.25 mM vitamin C (VitC ...
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bioRxiv - Neuroscience 2024Quote: ... one well of dissRO and iMG-dissRO were treated with recombinant human CCL2 (C-C Motif Chemokine Ligand 2, Peprotech, #300-04) at a final concentration of 10 ng/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Each diluted suspension was carefully mixed and further diluted to 2 ml using OPC differentiation media (antibiotic-free DMEM-Sato supplemented with 10 ng/ml CNTF (Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886 ...
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bioRxiv - Bioengineering 2024Quote: ... in T75 flasks at 2 million/mL and differentiated for 7-9 days with 50 ng/mL recombinant human M-CSF (300-25, PeproTech, Cranbury, NJ) and 20 ng/mL recombinant human IL-4 (200-04 ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were differentiated into primary human osteoclasts over 10 days, with media refreshed every 2-3 days and receptor activated nuclear factor κB ligand (RANKL, 25 ng/µL (PeproTech, London, UK) added on differentiation day 8 to stimulate osteoclast formation.
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bioRxiv - Biochemistry 2024Quote: ... the media was then exchanged for 2ml/well of mesoderm induction media (MIM), composed of STEMdiff APEL 2 Medium (STEMCELL, 05275) + 50ng/ml Recombinant Human FGF-basic (Peprotech, 100-18B), 50ng/ml Human BMP-4 (E.coli derived ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Developmental Biology 2021Quote: ... the SC solution was centrifuged at 300 g for 5 minutes to remove the supernatant and SFM containing 50 μg/ml stem cell factor (PeproTech, 300-07, Cranbury, NJ, USA), 100 μg/ml IL-3 (PeproTech ...
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bioRxiv - Microbiology 2023Quote: Confluent monolayers of MRC-5 and LEC were treated with 25 ng/mL recombinant human IFN-β and γ (Peprotech #300-02BC and #300-02) in the culture media ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... 2 million bone marrow cells were co-cultured with 1 × 105 GL261 cells in 6-well plates in 2 ml RPMI/10% FBS supplemented with 40 ng/ml GM-CSF and 80 ng/ml IL-13 (PeproTech, Rocky Hill, NJ) for 3-4 days ...
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bioRxiv - Cell Biology 2023Quote: ... neuromuscular co-cultured were gently covered with 2 mL of DM containing brain-derived neurotrophic factor (BDNF, 10 ng/mL, PeproTech 450-02-10μg) and glial cell-derived neurotrophic factor (GDNF ...
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bioRxiv - Microbiology 2023Quote: ... for 48h at a concentration of 1 bead/cell in the presence of 100 U/ml IL-2 (PeproTech, Inc., Cat. #200-02) in RPMI +/+ and were subsequently cultured under hypoxia (HOX - 1% O2 ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented by 10% BenchMark™ Fetal Bovine Serum (GeminiBio, 100-106), or ImmunoCult (STEMCELL, 10981) + 50 ng/mL IL-2 (PeproTech, 200-02). When indicated CAR-T were treated with CD38 inhibitor 1uM 78c (6391 ...
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bioRxiv - Immunology 2024Quote: ... Matrices were placed into ImmunoCult™-XF T Cell Expansion Medium (StemCell, Cat: 10981) supplemented with rhIL-2 (200IU; Peprotech, Cat: 200-02), rhIL-7 (10ng/mL ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were allowed to recover at a density of 1E6 cells/mL in antibiotic-free medium containing 100 IU/mL IL-2 (PeproTech, Rocky Hill, NJ) for 72 hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... and histone isolation procedures activated CD8+ Teff cells were re-cultured (5 × 106 cells/well in 6-well plates) for up to 48h in IMDM containing 50 U/mL IL-2 (PeproTech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Bioengineering 2024Quote: ... T-cells were maintained at a cell density of 106 cells mL-1 and supplemented with 50 IU mL-1 rh IL-2 (Peprotech, CAT#200-02) every 2 days ...
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bioRxiv - Immunology 2024Quote: ... human TGF-β1 (AF-100-21C) and murine IL-2 (AF-212-12) (200-23) were procured from PeproTech (Rocky Hill, NJ, USA). α-Minimal essential media (MEM ...
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bioRxiv - Immunology 2024Quote: ... at a 1:3 T cell:bead ratio cultured in R10 media and 20 IU of recombinant human IL-2 (Peprotech, Cat-no. 200-02). Cells were transduced with CAR lentivirus at a multiplicity of infection (MOI ...