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Citations for Peprotech :
1251 - 1300 of 1458 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Immunofluorescent staining was in general performed on HUVECS cultured on 12 mm glass coverslips coated with 5 μg/ml FN and treated with or without o/n TNFα (10ng/ml) (Peprotech), washed with PBS+/+(1mM CaCl2 and 0.5mM MgCl2) ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... adherent monocytes were differentiated into macrophages over a 5 days in the presence of 50 ng/mL GM-CSF (Peprotech) in RPMI supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Genomics 2020Quote: ... 1% Glutamax, and 1% FBS, 8 ng/ml FGF2, 5 ng/ml CNTF, and 10 ng/ml BMP4, from Peprotech). On day 7 ...
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bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Bioengineering 2022Quote: ... the spheroids were then transferred to spinal cord medium II (ScM II) with 10 nM retinoic acid and 5 ng/mL recombinant human BMP4 (Peprotech) for the next 6 days ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with phosphate buffered saline (PBS) and cultured in M231 SMDS medium supplemented with 5 ng/mL PDGF-BB (Peprotech) to provoke cell migration ...
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bioRxiv - Developmental Biology 2024Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL mSCF (Peprotech), 5 ng/mL Flt3-L ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Immunology 2024Quote: ... B cells were harvested and re-seeded on freshly irradiated 40LB cells in the presence of 5 ng/ml recombinant mouse IL-21 (PeproTech). On day 7 ...
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bioRxiv - Physiology 2024Quote: ... day 7 cells were dissociated with Accutase and plated at a density of ∼5 × 104 cells/cm2 in laminin-511 (PeproTech)-coated plates with podocyte induction media ...
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bioRxiv - Immunology 2022Quote: ... cells were allowed to proliferate for two additional days by activation with 50 U/ml IL-2 (Peprotech) and 10% HS and 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... in T cell medium supplemented with 2ng/ml TGFβ (R&D) and 100 U/ml rhIL-2 (PeproTech) in the presence of plate bound anti-CD3ε (1 μg/mL ...
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bioRxiv - Genetics 2021Quote: ... T cells were transferred to the culture medium supplemented with 30 IU/mL IL-2 (Peprotech, 200-02A). After electroporation for 3 days ...
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bioRxiv - Immunology 2021Quote: ... in the presence of human IL-2 (30 U/ml) and murine IL-7 (10 ng/ml; PeproTech) in RPMI 1640 media supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2021Quote: ... diluted in complete RPMI (cRPMI; RPMI supplemented with 10% FBS plus recombinant human IL-2 (20IU/ml; Peprotech), as previously described.65,66 Activated cells were incubated for six days with a media change on day three.
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bioRxiv - Immunology 2021Quote: ... in complete RMPI medium supplemented with the recombinant murine 1,000 U/ml IL-2 (PeproTech, Rocky Hill, NJ) and cultured for 5 days ...
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bioRxiv - Immunology 2021Quote: ... whole bone marrow was plated at 1M cells/mL in cRPMI + 400U/mL Proleukin human IL-2 (Peprotech). Fresh IL-2 was added every 3rd day ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1.0 x 106 cells mL-1 supplemented with IL-2 at 100IU mL-1(Peprotech). Each splitter plate condition was evaluated in triplicate ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1.0 x 106 cells mL-1 supplemented with IL-2 at 100IU mL-1(Peprotech). IL-2 at 100IU mL-1 was supplemented every 2 ~ 3 days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... clone OKT3) and anti-CD28 (eBiosciences, clone CD28.2) antibodies in IL-2-containg medium (0.01 ug/ml, Peprotech) for 4 days ...
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bioRxiv - Immunology 2023Quote: ... and expanded in RPMI 1640 containing 10% FBS and 100 IU/mL of recombinant human IL-2 (Peprotech). Human blood samples were enriched for T cells using negative bead selection (StemCell ...
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bioRxiv - Immunology 2022Quote: ... and 2 mM glutamine (no BCS) with or without 10 ng/ml TGF-β1 (PeproTech, Rocky Hill, NJ). After 48 hours ...
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bioRxiv - Immunology 2024Quote: ... CD8+ primary T cells were cultured in complete RPMI supplemented with/without 50U/ml of IL-2 (Peprotech). In primary cell experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 μg/mL recombinant human relaxin-2 in 0.1% BSA-PBS (Rln, R; Peprotech, Rocky Hill, NJ, USA) and a combination of E+R for the specified amount of time ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured in NK MACS medium (Miltenyi) supplemented with recombinant human IL-2 1000 U/mL (Peprotech). Cells were activated over 7 days with anti-CD2 and anti-NKp46-coated beads (Miltenyi ...
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bioRxiv - Biophysics 2024Quote: ... Activated T cells were cultured in R10 medium substituted with 10 ng/ml IL-2 (PeproTech, 212-12) for up to 14 days ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... yolk sac embryoid bodies (YS-EBs) were generated by treating EBs with bone morphogenetic protein 4 (BMP4, 50 ng/ml, Peprotech; to induce mesoderm), stem cell factor (SCF ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cell Biology 2021Quote: ... Serum-starved cells were pretreated with 20 nM aPC (Hematologic Technologies, #HCAPC-0080) for 4 h and then incubated with 25 ng/mL TNF-α (PeproTech, #300-01A) for 24 h ...
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bioRxiv - Immunology 2023Quote: ... or 12 well plates and treated with 50 ng/mL of IFN-γ (R & D, 485-MI) or 25 ng/mL of IL-4 (PeproTech, 214-14) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from hiPSCs and induced to yolk sac EBs (YS-EBs) by adding bone morphogenetic protein 4 (BMP4, 50 ng/mL, Peprotech; to induce mesoderm), vascular endothelial growth factor (VEGF ...
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bioRxiv - Immunology 2024Quote: ... 1×105 BMDM were added to iDpt;R26YFP-CTRL and iDpt;R26YFP;Csf1fl/f Exons 4–6 fibroblasts or plated in the presence/absence of M-CSF (Peprotech; Cat# 315-02). The 96-well plate was placed on the Incucyte SX5 (Sartorius) ...
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bioRxiv - Molecular Biology 2020Quote: ... NPCs were cultured for up to 5 passages on Matrigel with neural induction media supplemented with 40 ng/ml bFGF (Peprotech: (AF)100-18B) ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: Wild-type mouse colonic organoids were plated onto 24-well plates in Matrigel and allowed to culture for 5 days before treatment with recombinant murine INF-y (PeproTech, UK) at concentrations of 0.2 ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... T cells were activated in the presence or absence of recombinant mouse IL-6 (5, 10, 50, 100 ng/mL, #AF-216-16, PeproTech, US) for 0 ...