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Citations for Peprotech :
1251 - 1300 of 1397 citations for Recombinant Rat IL1B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... macrophages were cultured in macrophage media supplemented with 20 ng/ml human recombinant IFN-γ (PeproTech, catalog no. 300-02) and 25 ng/ml LPS (Invivogen ...
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bioRxiv - Immunology 2024Quote: ... The medium was also supplemented with 10 U/ml of DNaseI and 2 ng/mL of recombinant IL-15 (Peprotech). The cell suspension was transferred to a T25 flask (Corning ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Pathology 2023Quote: ... Bone marrow was collected and cultured in RPMI-1640 complete medium (CM) containing 20ng/ml recombinant mouse GM-CSF (Peprotech). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Physiology 2023Quote: ... protein from transfected THP-1-derived macrophages and human cardiac fibroblasts treated with macrophage supernatant or with recombinant human HB-EGF (100 ng/ml, 30min, #100-47, PeproTech) was isolated ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Primary Keratinocytes from three donors were seeded in 24-well plates and stimulated with KGM Media supplemented either with 0.1 ng/ml or 10 ng/ml of recombinant human IFN-γ (PeproTech), or infected with T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Bioengineering 2023Quote: ... At Day 11 the cytokine composition of the media was changed to 10ng/μl of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/μl (Peprotech). From Day 15 onwards ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 a ...
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bioRxiv - Cancer Biology 2023Quote: ... These tumoroids were then cultured in BEN selection media (basal medium with mouse recombinant EGF (50 ng/ml) and Noggin (100 ng/ml) (PeproTech) without R-spondin ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then cultured with and without 14 x 103 LNCs/well and treated with 10 ng/mL recombinant murine IFNγ (Peprotech), 100 nM PD-1/PD-L1 inhibitor (Thomas Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Immunology 2024Quote: ... Cells were plated in DMEM containing 10% v/v FBS and 10 ng/ml recombinant M-CSF (Peprotech #315-02). Cells were plated at a concentration of ∼150,000 cells per cm2 on non- treated Petri plates ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were assayed immediately or cultured overnight in StemSpan SFEM (STEMCELL) with 50 ng/mL recombinant human stem cell factor (PeproTech), 10-6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... On day 11 the cytokine composition of the media was changed to 10ng/ml of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/ml (Peprotech). From day 15 onwards ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow was stimulated with recombinant murine IL-3 (10 ng/mL) and IL-6 (10 ng/mL) (Peprotech, 21616) for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... 1×106 differentiated iRBCs or undifferentiated HUDEP-2 cells were stimulated with 50 ng/mL recombinant human interferon-gamma (PeproTech) for 6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Immunology 2024Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech).
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...
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bioRxiv - Cell Biology 2020Quote: ... with 500 nM SB431542 (Selleckchem, cat. no. S1067) and 20 ng/ml recombinant human (rh)Noggin (Peprotech, cat. no. 120-10C). On days 5-9 ...
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bioRxiv - Immunology 2021Quote: Flow-cytometry-purified BM-eos (B6J) were conditioned overnight with colon supernatant and treated with recombinant mouse IFN-γ (10ng/ml, 315-05 PeproTech), as indicated ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with 25 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 24 hours.
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bioRxiv - Immunology 2021Quote: ... Th17 cell cultures received 5 ng/mL TGF-β and 50 ng/mL IL-6 recombinant and 20 µg/mL anti-IL-4 and 100 µg anti-IFN-γ (PeproTech). After 48 h ...
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bioRxiv - Cancer Biology 2019Quote: ... 20ng/mL human recombinant epidermal growth factor (EGF) and 20ng/mL basic broblastic growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ).
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bioRxiv - Biochemistry 2021Quote: Recombinant human platelet-derived growth factor-BB (PDGF-BB) and recombinant human transforming Growth Factor-β1 (TGFβ1; HEK293 derived) were purchased from PeproTech (Hamburg, Germany) and they were dissolved in sterile ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates in 100 µl media per well at a density of 40,000 cells/ml one day prior to treatment with soluble recombinant TRAIL (rTRAIL; Peprotech-310-04) or MEDI3039 (Medimmune) ...
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bioRxiv - Cell Biology 2021Quote: ... penicillin/streptomycin (50 IU/mL penicillin and 50 μg/mL streptomycin) and 10 pg/μL recombinant murine macrophage colony stimulating factor (PeproTech, Inc.).
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Immunology 2019Quote: ... 1ml of cell suspension was plated per well in tissue culture-treated 24-well plates and supplemented with 20ng/ml recombinant GM-CSF and 5ng/ml TGFβ (Peprotech, USA). Cells were cultured at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
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Combination image flow cytometry reveals novel methods for isolating megakaryocyte progenitors cellsbioRxiv - Cell Biology 2019Quote: ... 1×107 cells were incubated in IMDM + 10% FBS supplemented with 50 ng/ml of recombinant murine TPO (PeproTech, #315-14) in a T75 flask at 37 °C in 5% CO2 for 72 h.
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... blasts were pre-treated for 48 hours with Recombinant Human IFN-γ at a final concentration of 200 U/mL (cat#300-02 from PeproTech). Cells were cultured in IMDM supplemented with 1% L-glutamine (G) ...
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bioRxiv - Cancer Biology 2022Quote: Wild-type mouse colonic organoids were plated onto 24-well plates in Matrigel and allowed to culture for 5 days before treatment with recombinant murine INF-y (PeproTech, UK) at concentrations of 0.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μM of A83-01 (HY10432, MCE, USA) and 20 ng/mL of recombinant human epidermal growth factor (EGF; AF100-15, PeproTech, USA), 10% KSR and 1% NEAA ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 splenocytes from single cell suspensions were incubated with increasing concentrations of recombinant mouse IFNγ (0, 0.1, 1, 10, 100 ng/mL) for 48 hours (Peprotech, 315-05). Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504) ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of spleens from 10-week-old Foxp3Thy1.1 mice were stimulated for 15 minutes at 37°C with the indicated concentration of recombinant mouse IL2 (PeproTech, 212-12) in serum-free RPMI 1640 ...