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Citations for Peprotech :
1151 - 1200 of 1397 citations for Recombinant Rat IL1B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... This medium was further supplemented with vitamins and minerals (Table 4), thyroxine (Whitney et al., 2018) and recombinant human TGFβ1 (Peprotech). Medium sampling ...
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech) at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Two days later cells were passaged and cultured in fresh media supplemented with (12.5U/ml) recombinant IL-2 (PeproTech). Cells were passaged again after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... with sorted autologous TFH cells (at a 1:1 ratio) or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21 ...
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bioRxiv - Biochemistry 2022Quote: ... lung organoids were cultured in medium including recombinant human transforming growth factor (TGF)-β1 (Peprotech, Rocky Hill, NJ, USA) without A83-01 (Tocris ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... normalized live parasites (1×106) or heterologously expressed GP63 was incubated with 500pg/ml of human recombinant CXCL10 (Peprotech) for the indicated time at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... cells were stimulated with either 50 ng·ml−1 recombinant hVEGF-165 for 5 min (Peprotech, Rocky Hill, NJ, USA), 20 ng·ml−1 recombinant hPDGF-B for 5 min (Peprotech ...
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bioRxiv - Neuroscience 2021Quote: ... and the pellet resuspended in 12 mL of DMEM media containing 10 ng/mL recombinant murine M-CSF (Peprotech). Cell suspensions were then pooled as required and placed in a 100 mm x 20 mm (diameter x height ...
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bioRxiv - Neuroscience 2021Quote: ... organoids were also generated as described excepting the addition of 25ng/mL recombinant human PTN (Peprotech, CAT#: 450-15) into differentiation media for 7d.
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Immunology 2022Quote: ... heterologous Human naive T cells from healthy volunteers were sorted as CD3+CD4+CD45RA+CD45RO- and added to the culture at a ratio of 1:10 (DC:T cell) in the presence of IL-2 recombinant cytokine (50 ng/ml) (Peprotech). Five days post co-culture ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
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bioRxiv - Bioengineering 2024Quote: ... SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium) ...
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bioRxiv - Immunology 2024Quote: CD8 T cells from WT p14 or Irf5-/- p14 mice were isolated as described above and cultured with or without 1µg/ml gp33 peptide in complete RPMI medium supplemented with 2ng/ml recombinant human IL-2 (Peprotech). Cells were then collected at 6 or 24 hours after incubation and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... tumor suspension and PBMCs were thawed and plated with recombinant human IFNy at 200 ng/mL (Peprotech, #300-02) (only for tumor suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... Three weeks after transplantation mice were treated with 500 ng/mouse/day of recombinant IL1β (Peprotech, Cranbury, New Jersey) intraperitoneally ...
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bioRxiv - Immunology 2022Quote: ... followed by addition of 100,000 sorted CD5- or CD71- DN3 thymocytes per well together with 10 ng/ml recombinant mouse IL-7 (PeproTech).
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bioRxiv - Immunology 2022Quote: ... or (15 ng/mouse) of recombinant-mouse IFN-γ (rIFN-γ expressed in E. coli; PeproTech, Rocky Hill, NJ), i.p ...
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bioRxiv - Immunology 2023Quote: ... 6-week-old Foxp3IRES-GFP mice were injected intravenously with 10 μg of recombinant mouse IL2 (PeproTech, 212-12) or PBS vehicle control (in 110 μl volume) ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant human IL-6 (AF-200-06) and human TNFα (300-01A) were purchased from PeproTech (New Jersey, USA). Mouse cTnT/TNNT2 ELISA Kit (E-EL-M1801c) ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations were centered in the nanomolar range previously used to study acute cytokine responses in neuron cultures.23,89 Recombinant murine cytokines were purchased from Peprotech, IFN-γ (cat 315-05) ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in differentiation medium I-10 + M-CSF (i.e., IMDM, 10 % FBS, 1 mg/ml Pen/Strep containing 25 ng/ml recombinant murine M-CSF (PeproTech)) at a density of 1-2 Mio ...
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bioRxiv - Immunology 2023Quote: ... at a density of 7.5×105 cells/ml in 2 ml complete media supplemented with 20 ng/ml recombinant human GM-CSF (Peprotech). The plates were incubated at 37°C in 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... naive primary human CD4+ T cells were cultured on-chip for 24 h with media spiked with 1.22 ng/mL of recombinant human CCL21 (Peprotech). Brightfield images were acquired using transmitted light in time series mode (1 image was collected every 30 sec for 10 cycles ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Cell Biology 2024Quote: ... Bone marrow-derived macrophages (BMDMs) were differentiated using 20 ng/ml of recombinant murine macrophage colony-stimulating factor (PeproTech) from bone marrow cells which were harvested from femurs and tibias ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were re-suspended in DMEM/F12 complete medium containing 20 ng/mL recombinant murine FGF (PeproTech, 450-33), 20 ng/mL recombinant murine EGF (PeproTech ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Microbiology 2020Quote: ... the PBMCs were cultured with the synthetic peptide mixture of SARS-CoV-2 at a concentration of 1μg/ml or DMSO in RPMI 1640 medium containing 10% FCS and 20 U/ml recombinant human IL-2 (Peprotech) for 7 days ...
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bioRxiv - Microbiology 2020Quote: ... were cocultured with 2×105 peptide-pulsed autologous DCs in a 24-well plate in the presence of 10 ng/mL recombinant human interleukin-2 (IL-2; Peprotech). The next day ...
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bioRxiv - Immunology 2019Quote: Bone marrow-derived macrophages (BMDMs) were cultured in complete RPMI with recombinant human CSF-1 (50 ng/ml; PeproTech Inc.) for 6 days ...
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bioRxiv - Biochemistry 2019Quote: ... CD14+ cells were differentiated in to macrophages using recombinant human granulocyte-macrophage colony-stimulating factor (200 ng/mL GM-CSF) and recombinant human interferon gamma (50 ng/ml IFNγ) (Peprotech) in standard tissue culture DMEM media containing fetal calf serum ...
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bioRxiv - Neuroscience 2019Quote: ... or a combination of lipopolysaccharide (LPS, 100 ng/mL) and recombinant murine IFN□ (25 ng/mL, PeproTech, Rocky Hill, NJ). Concurrently ...
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bioRxiv - Microbiology 2019Quote: ... and maintained as previously described[2] in media supplemented with 10ng/ml recombinant interleukin-4 and 10ng/ml granulocyte-macrophage colony stimulating factor (Peprotech). Bone marrow macrophages (BMMΦ ...
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bioRxiv - Microbiology 2019Quote: ... Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech) at a cell density of 2e6 cells/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were allowed to attach on coverslip for 1 h and then cells were incubated with murine recombinant CCL2 (Peprotech) for 5 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... and vascular endothelial growth factor (VEGF) (Catalog No. 100-20, Recombinant Human VEGF165, Peprotech, Rocky Hill, NJ, 0.1 μg/mL). A mixture of heparin ...
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bioRxiv - Bioengineering 2021Quote: Lyophilized 90 % PEGDMA microrods were loaded by resuspending ∼100,000 (100 k) microrod aliquots in 20 μL of 1 mg/mL recombinant human β-NGF (Peprotech) as described in the Protein loading of PEGDMA microrods section ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 anti-CD19 CD8 CAR T cells were expanded in presence of 7 x 106 irradiated (8000 cGy) TM-LCL feeder cells in RPMI1640 containing 50 IU/mL human recombinant IL-2 (Peprotech), 10 ng/ml recombinant human lL-15 (Peprotech) ...
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bioRxiv - Cell Biology 2020Quote: ... Enteroendocrine cell reporter organoids were derived from the proximal small intestine of Neurog3-RFP mice (expressing RFP under the Neurog3 promoter) and cultured as described above using recombinant murine Noggin (100ng/ml, Peprotech) and 10% R-Spondin conditioned medium ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells at a plate confluence of 80% were treated for 10 min with 100 ng/mL animal-free recombinant human EGF (PeproTech) or Gibco™ distilled water (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: PDAC cells were seeded in a 6-well plate and after 24 hours were treated with 10 ng/ml of recombinant TGF-β1 (rTGF-β1, Peprotech) either alone or in the presence of 80 μM of Vactosertib (Vacto ...