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Citations for Peprotech :
751 - 800 of 908 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Differentiation of ReN cells was performed by replacing FGF and EGF with 2 ng/ml GDNF (Peprotech, 450–10) and 1 mM dibutyryl-cAMP (Invivochem ...
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bioRxiv - Immunology 2024Quote: ... activated OT-1 T cells were initially expanded in IL-2 (20ng/ml) and IL-12 (20ng/ml; Peprotech) before being switched into IL-2 alone ...
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bioRxiv - Immunology 2024Quote: ... cells from C57BL/6 and CD11cdnR spleens were cultured for 7 days with 10 ng/ml IL-15 (PeproTech) supplemented with or without 10 ng/ml TGFβ1 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... The bone marrow cell suspension was cultured on non-treated 150-mm Petri dishes at a concentration of 0.5×106 cells/ml in complete RPMI-1640 with 20 ng/ml rGM-CSF (PeproTech). On day 3 ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and cultured in medium supplemented with mouse rIL-2 (20 U/ml; cat. 212-12, Peprotech) for 4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were re-suspended in DMEM/F12 complete medium containing 20 ng/mL recombinant murine FGF (PeproTech, 450-33), 20 ng/mL recombinant murine EGF (PeproTech ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were maintained in complete RPMI (10% FBS, 1% Pen/strep) with 30 U/mL rIL-2 (Peprotech, 200-02). To infect ...
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bioRxiv - Immunology 2019Quote: ... Bone marrow cells were then plated at 106 cells/ml in cRPMI containing 20ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF, Peprotech), and cultured for 7 days ...
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bioRxiv - Systems Biology 2019Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL SCF (Peprotech), 5 ng/mL Flt3-L ...
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bioRxiv - Developmental Biology 2020Quote: ... Resuspended cells were filtered with 35 µm filter (Falcon) and sorted into sorting buffer (20 ng/ml human basic FGF (Peprotech), 1XB27 RA-(Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... in the presence of freshly added 20 ng/ml mouse glial cell line-derived neurotrophic factor (GDNF, PeproTech, 450-44) as detailed elsewhere (Sleigh et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 anti-CD19 CD8 CAR T cells were expanded in presence of 7 x 106 irradiated (8000 cGy) TM-LCL feeder cells in RPMI1640 containing 50 IU/mL human recombinant IL-2 (Peprotech), 10 ng/ml recombinant human lL-15 (Peprotech) ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
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bioRxiv - Biochemistry 2020Quote: ... At day 8 cells were detached and aggregated in the U bottom microwells in the presence of 20 ng/mL HGF (PeproTech), 10 ng/mL oncostatin M (OSM ...
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bioRxiv - Immunology 2019Quote: ... One week later cells were harvested and seeded on well plates and incubated for one day with GM-CSF (20 ng/mL; Peprotech). Training experiments were performed with these cells according to the Monocyte training protocol ...
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bioRxiv - Immunology 2022Quote: ... the cells were supplemented with murine rIFN-γ (40 μg/ml) (Recombinant Murine IFN-γ, Catalog Number:315-05, Peprotech) or were kept unaltered ...
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bioRxiv - Immunology 2022Quote: ... The cells were cultured in the presence of 20 ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF; from Peprotech) in complete DC medium (CDCM) ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Neuroscience 2019Quote: ... to a density of 220 000 cells/cm2 in N2B27 Medium containing 10 μM Rock inhibitor and 20 ng/ml FGF2 (Peprotech). The medium was changed every third day without Rock inhibitor ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were grown to at least 500,000 cells/mL before treatment with 20 ng/mL recombinant human tumor necrosis factor α (TNF) (Peprotech), 300 nM A-485 (Structural Genomics Consortium) ...
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bioRxiv - Cancer Biology 2021Quote: ... and then cultured in CTS T Cell Expansion medium (Thermo) containing 10% FBS and 100 IU/ml human IL-2 (PeproTech). The CellTiter 96 MTS assay (Promega ...
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bioRxiv - Genetics 2019Quote: ... monocytes were plated in a 100mm x 20mm dish and cells were treated with 800 U/ml M-CSF (PeproTech) for seven days ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
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bioRxiv - Immunology 2021Quote: ... Optimized TCR constructs were retrovirally transduced in CD8+ healthy donor derived T cells as described before and expanded for 7 to 10 days with IL-7 and IL-15 (5ng/mL; Peprotech) before functional characterization (13,14).
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bioRxiv - Molecular Biology 2021Quote: ... in 96-well plates (5.0 x 105 cells/well) and incubated with DMEM containing 10% FCS in the presence of 100 ng/ml recombinant murine RANKL (PeproTech) with 20 ng/ml recombinant murine M-CSF (PeproTech) ...
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bioRxiv - Molecular Biology 2021Quote: ... with subsequent maintenance of adherent cells in RPMI 1640 medium supplemented with 10% human serum and M-CSF (10 ng/ml, Peprotech) for 3 days and then differentiated for a further 4 days in RPMI 1640 medium supplemented with 10% fetal calf sera without M-CSF.
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bioRxiv - Molecular Biology 2021Quote: ... cells were dissociated and 3×106 cells were plated in a bacterial petri dish in N2B27 medium supplemented with EGF 10 ng/mL (Peprotech) and FGF2 10 ng/mL (R&D systems) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 100 ng/ml recombinant human FLT3-L (Peprotech). After three days of differentiation ...
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bioRxiv - Immunology 2022Quote: Primary neuronal cells (DIV14) or oligodendrocytes were stimulated with 100 ng/μl of recombinant murine IL-12p70 (#210-12, Peprotech) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... primary neuronal cells were stimulated with 30 ng/μl or 100 ng/μl of Recombinant Murine IL-12p70 (#210-12, Peprotech) for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Cell Biology 2022Quote: ... 1.5 x 105 cells were seeded on collagen-coated 60 mm dishes and treated daily with 200 pM TGF-β1 (100-21; PeproTech) dissolved in DMEM media with 1% BSA and 1% penicillin/streptomycin for 4 days ...
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bioRxiv - Bioengineering 2022Quote: ... and then for three to five days (plasmablast/plasma cell differentiation) in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... TCR condition – cells activated with anti-CD3/CD28 antibodies on day 0 and cultured with IL-2 (2 ng/ml, PeproTech); TCR+TCR condition – cells activated with anti-CD3/CD28 on day 0 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured in RPMI 1640 medium with 10% FBS containing GM-CSF (25 ng/ml; Peprotech, Rocky Hill, NJ) and IL-4 (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The iPSC colonies were maintained in undifferentiated pluripotent state by culturing the cells under feeder free conditions on 0.1% Geltrex® (Peprotech) coated culture plates ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
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bioRxiv - Cell Biology 2023Quote: ... 1x106 cells were plated per well of 96-well round bottom plate and treated with 10ng/mL of TGF-β1 (Peprotech) or equivalent volume of DMSO for 20 minutes at 37°C ...