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Citations for Peprotech :
701 - 750 of 908 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Maintenance of neural progenitor cells was performed in neural maintenance medium with 10 µM bFGF (Peprotech, 100-18C) added after subculturing ...
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bioRxiv - Immunology 2024Quote: ... Single cell clones were expanded and stimulated with 1 ng/mL recombinant murine IFNγ (Peprotech, Cat. 315-05). Clones were stained for MHC-I H2-Db expression by flow cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... The NK cells were counted and were resuspended in cRPMI supplemented with 5 ng/ml rhIL-15 (PeproTech) and rested overnight in a 96-well U-bottom plate at 37 °C and 5 % CO2.
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bioRxiv - Immunology 2023Quote: ... BM cells from C57BL/6J mice were stimulated with 40 ng/mL recombinant GM-CSF (Peprotech, NJ, USA) for 4 days ...
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bioRxiv - Physiology 2023Quote: ... Hep3B and HepG2 cells and primary hepartocytes were treated with 10 or 25 ng/ml of BMP6 (PeproTech) or BMPs 2 ...
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bioRxiv - Immunology 2023Quote: ... NK cells were cultured for 6 hours in the presence of 1 ng/ml murine IL-12 (Peprotech) and 1 ng/ml murine IL-18 (MBL) ...
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bioRxiv - Molecular Biology 2023Quote: ... macrophages were generated from adherent cells in RPMI-1640 containing 10% FCS and M-CSF (Peprotech, 315-02) (30 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... splenic and lymph node single cell suspensions were cultured with Interleukin 7 (IL-7) (5 ng/ml, PeproTech) in complete RPMI 1640 medium ...
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bioRxiv - Molecular Biology 2024Quote: ... Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1, Peprotech) and anti-mouse CD40 antibody (5 µg ml-1 ...
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bioRxiv - Immunology 2024Quote: Human TNF-α was quantified in cell supernatants using a commercially available ELISA kit from PeproTech (900-TM25) and the appropriate commercial buffers (TMB ELISA Buffer Kit ...
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bioRxiv - Immunology 2024Quote: ... A portion of the mixed naïve T cells maintained in recombinant mouse IL-7 (5 ng/mL, Peprotech) to verify baseline T cell ratios over time in culture.
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bioRxiv - Immunology 2020Quote: ... GM-BMDMs were generated by culturing non-adherent BM cells in cDMEM supplemented with 50ng/mL GM-CSF (Peprotech). CD122+BMDMs were generated by adding 1ng/mL IFNα (Biolegend ...
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bioRxiv - Genomics 2020Quote: ... cells were assessed with and without 16h pretreatment of 10 ng/ml recombinant human IFNγ (Peprotech, Rocky Hill, NJ).
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bioRxiv - Cell Biology 2021Quote: ... PU.1 eYFP low and high SLAM cells were purified from mice that were treated with either PBS or .5µg IL-1β (Peprotech) 24 hours prior to harvest ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 500 U/mL human IFNγ and human IFNβ for 2 days prior to isolation (Peprotech). For all lysate blots ...
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bioRxiv - Cell Biology 2021Quote: ... These cells were then activated using CD3 and CD28 antibodies in the presence of 100U/ml IL-2 (Peprotech). For reprogramming ...
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bioRxiv - Cancer Biology 2022Quote: ... with sorted autologous TFH cells (at a 1:1 ratio) or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21 ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly sorted Lin-CD150+CD48-bone marrow cells were seeded at 3,000 cells per well in 96 well plates and cultured in SFEM supplemented with 10 ng/mL IL3 (Peprotech), 10 ng/mL IL6 (Peprotech) ...
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bioRxiv - Biophysics 2022Quote: ... EMT was induced by incubating cells in medium supplemented with 2 ng/mL TGF-β1 (PN:100-21, Peprotech) for 72 hours prior to the measurement ...
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bioRxiv - Cancer Biology 2021Quote: ... For inhibitor experiments, LGG275 cells were treated with γ-secretase inhibitors (DAPT (GSI-IX, Selleck Chem) and LY411575 (Peprotech)) at 10 mM for 5 days before RNA was extracted for QPCR ...
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bioRxiv - Neuroscience 2022Quote: ... coated 6 well plates at 200,000 cells/well in 2 ml/well StemFlex supplemented with ROCK inhibitor Y-27632 (5 mM, PeproTech) to inhibit apoptosis ...
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bioRxiv - Developmental Biology 2022Quote: ... undifferentiated WT ESCs were seeded at a density of 250.000 cells per well in 6-well plates and treated with 200ng/mL Wnt antagonist Dickkopf 1 (DKK1) (Peprotech) or 10μM iCRT3 [inhibitor of β-catenin–responsive transcription] (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then pipetted into penicillin-streptomycin free StemSpan SFEM medium with thrombopoietin (TPO; 20 ng/mL; PeproTech) and stem cell factor (SCF ...
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bioRxiv - Plant Biology 2019Quote: ... cells were stimulated with either 50 ng·ml−1 recombinant hVEGF-165 for 5 min (Peprotech, Rocky Hill, NJ, USA), 20 ng·ml−1 recombinant hPDGF-B for 5 min (Peprotech ...
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bioRxiv - Bioengineering 2019Quote: ... at a concentration of approximately 8.0 × 105 cells mL−1 with 100 IU mL−1 IL-2 (200-02, Peprotech) and cultured in 6 well non-TC treated plates (Corning ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were re-plated using Accutase and seeded at a density of 100,000 cells/cm2 onto Geltrex-coated plates in N2B27 medium supplemented with BDNF (20ng/ml, Peprotech), GDNF (20ng/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were re-plated using Accutase and seeded at a density of 100,000 cells/cm2 onto Geltrex-coated plates in N2B27 medium supplemented with BDNF (20ng/ml, Peprotech), GDNF (20ng/ml ...
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bioRxiv - Immunology 2019Quote: ... Cells were resuspended at 106/ml in cRPMI with 20ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF, Peprotech) and plated for culture ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 million cells were plated into 96-well U-bottom plate with 50 ng/ml IL7 (PeproTech, 217-17) and 50 ng/ml IL12 (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were polarized into M1 macrophages by 20 h incubation with human interferon (IFN)-γ (PeproTech, 20 ng/mL) and ultrapure LPS (10 pg/mL ...
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bioRxiv - Immunology 2021Quote: ... and plated in 6-well cell culture plates at the concentration of 1.5 million cells per mL in 4mL total of complete IMDM medium supplemented with FLT3L (200ng/ml, Peprotech). At day 10 of differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... Monocytes (3.6 ×106 cells) were first seeded and stimulated with M-CSF for 3 days (20 ng/ml, PeproTech) in 1-well chamber slides (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... cells were exposed to different dose-course or time-course of human IL-22 (Peprotech, Rocky Hill, NJ, USA). In order to study the rescue effecting ...
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bioRxiv - Immunology 2022Quote: ... heterologous Human naive T cells from healthy volunteers were sorted as CD3+CD4+CD45RA+CD45RO- and added to the culture at a ratio of 1:10 (DC:T cell) in the presence of IL-2 recombinant cytokine (50 ng/ml) (Peprotech). Five days post co-culture ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with TNF-α (100 ng/ml) and IFN-γ (10 ng/ml, all Peprotech, London, UK) [70] for 24 hours in reduced medium (basal EBM2 (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: K562 cells were cultured in 12-well plates and treated with 10 ng/mL interferon beta (PeproTech, #300-02BC) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
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bioRxiv - Immunology 2024Quote: ... Edited cells were cultured and expanded in R10 containing 5ng/ml IL7 and 5ng/ml IL15 (Peprotech #20007, #20015) and replenished every 2-3 days until freezing at days 14-16.
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bioRxiv - Molecular Biology 2023Quote: ... and then differentiated the cells into macrophages by supplementing with M-CSF (20 ng/mL, Peprotech, Rocky Hill, NJ). Seahorse stress testing was performed to compare their metabolic glycolytic activity after 7 days of differentiation.
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bioRxiv - Physiology 2023Quote: ... Cells were then incubated for 24 hr in the presence or absence of 10ng/ml TNF (PeproTech, 315-01A) with or without 0.1uM 6-Hydroxyhexanoic acid (6-HHA ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR and 500 ng/mL fibroblast growth factor 4 (FGF4, Peprotech, Cranbury, NJ) in RPMI+B27 media (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were differentiated into neutrophils by replacing IL3 with 10ng/ml Granulocyte Colony Stimulating Factor (GCSF; Peprotech, 300-23) and inducing with 100nM 4-hydroxy-tamoxifen (OHT ...
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bioRxiv - Immunology 2023Quote: ... transduced T cells were rested for additional 2 days in fresh medium containing 10 ng/ml rhIL-2 (Peprotech) before 1×105 pMIG-empty vector (Ametrine+ ...
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bioRxiv - Cancer Biology 2022Quote: ... CML CD34+ cells were cultured at 100,000 cells/ml in SFM medium with the addition of EPO (3 U/ml, catalogue no. 100-64; PeproTech). Following a 72h incubation ...
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bioRxiv - Immunology 2023Quote: ... The cells were stimulated with IL-12+IL-18 (10 ng/ml, Peprotech, and 50 ng/ml, MBL, respectively) or plate-bound anti-CD16 (1 mg/ml ...
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bioRxiv - Genomics 2023Quote: ... Cells were differentiated into neutrophils by replacing IL3 with 10ng/ml Granulocyte Colony Stimulating Factor (GCSF; Peprotech, 300-23) and inducing with 100nM OHT after 48 hours.
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bioRxiv - Cell Biology 2023Quote: ... The unattached cells were collected and plated in 96-well plates with MCSF (50ng/ml, PeproTech, Rocky hill, USA)18added ...
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bioRxiv - Neuroscience 2024Quote: ... Differentiation of ReN cells was performed by replacing FGF and EGF with 2 ng/ml GDNF (Peprotech, 450–10) and 1 mM dibutyryl-cAMP (Invivochem ...