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Citations for Peprotech :
551 - 600 of 631 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... ROSAKIT WT cells were seeded at 0.3 x 106 cells/ml and treated for 4 days with IL-4 (20 ng/ml, PeproTech, #200-04) and human IgE (1 µg/ml ...
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bioRxiv - Immunology 2023Quote: EndoC-βH1 cells and dispersed human islets were exposed or not to IFNα (2000 U/mL, PeproTech Inc., Rocky Hill, NJ), IFNγ (1000 U/mL ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The cells were collected and cultured in X-Vivo (BE02-060F, Ozyme, France) supplemented with 50 ng/mL M-CSF (Peprotech, France) at a density of 2 x105 cells/mL ...
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bioRxiv - Immunology 2023Quote: ... Transfected cells were incubated for 24 hours before treatment with 50 ng/mL recombinant human IFN-γ (Peprotech; cat # 300-02) or cDMEM only control for 24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and plated on poly-L-ornithine/laminin (PLO)-coated dishes at a density of 10,000–15,000 cells/cm2 in N2B27 medium supplemented with 100 ng/ml FGF-8 (PeproTech, Cat.100-25), 200 ng/ml sonic hedgehog (PeproTech ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for the indicated time in the absence or presence of IL-7 (10 ng/ml, Peprotech, Cat#: 200-07) or stem cell factor (20 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Biochemistry 2019Quote: ... at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech, Rocky Hill, NJ). Bone-marrow-derived mast cells were prepared as described previously (55) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in 10 cm diameter Petri dishes with 10 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech, London, UK) for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions of cell lines were suspended at a density of 2,000 cells/ml in DMEM/Ham’s F-12 (nacalai tesque, no. 11581-15) containing 20 ng/ml bFGF (Peprotech, no. 100-18B), 20 ng/ml EGF (Wako ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... 33010-018)-coated plates at a density of 30 000 cells/cm2 and by supplementing the N2B27 medium with 12 ng/ml of bFGF (Peprotech, 100-18B) and 20 ng/ml of activin A (Peprotech ...
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bioRxiv - Immunology 2019Quote: ... Samples were adjusted to 7.5×105 cells/mL with cold cRPMI_M supplemented with 25 ng/mL of recombinant mouse colony-stimulating factor (m-csf, Peprotech, Rocky Hill, NJ) and cultured at 37°C ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2021Quote: ... Purified microglia cells were span down at 300 × g and plated at 100 103 cells per well in microglia media supplemented with 100 ng/ml of rhIL34 (Peprotech, 200-34), 2 ng/ml TGFb2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Developmental Biology 2021Quote: ... Differences were that for mouse cells the medium for aNSCs was additionally supplemented with 20 ng/ml recombinant murine Epidermal Growth Factor (PeproTech 315-09) and qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Immunology 2020Quote: ... and PD-L1 were performed as follows: 2e8 cells of the knockout (KO) library was stimulated with IFNγ (10ng/mL; Peprotech 315-05) for 24 hours after which cells were harvested by scraping to ensure integrity of cell surface proteins ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine stimulation of human articular chondrocytes was performed on cells at confluence by adding 10ng/ml interleukin-1β (IL-1β, Peprotech, London UK) to standard media and stimulating cells for up to 6 hours.
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bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Genomics 2023Quote: ... with cells in the third or subsequent passages defined as ‘late hCNCC’ and cultured with added 50 pg/ml BMP2 (Peprotech, 120-02) and 3 uM CHIR 99021 (Selleck ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2023Quote: Jurkat and THP1 WT and ARPC5 KO cells were seeded in 96-well plate and stimulated with RPMI 1640 + 100 ng/ml CXCL12 (Peprotech, 300-28A) or RPMI 1640 for the indicated time points ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Biophysics 2021Quote: ... EMT in all cells lines were induced by addition of 5ng/ml Targeted Growth Factor-β1 (TGFβ1) (Peprotech, catalog no. 100-21-10UG) for 48 hours62.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...