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Citations for Peprotech :
501 - 550 of 631 citations for Primary Bovine Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The iPSC colonies were maintained in undifferentiated pluripotent state by culturing the cells under feeder free conditions on 0.1% Geltrex® (Peprotech) coated culture plates ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Molecular Biology 2022Quote: ... Both SFEM II and our custom expansion media were supplemented with 100 ng/mL stem cell factor (Peprotech, 250-03), 40 ng/mL insulin-like growth factor (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and light zone cells (CD19+GL7+FAS+CXCR4-CD86+) and cultured in the presence of 200 ng/ml Il-9 (PeproTech) in RPMI media for 3 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were labelled with cell trace violet (CTV) (ThermoFischer Scientific) and cultured for 48 h with 10 ng/ml rhIL-2 (Peprotech) in vitro ...
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bioRxiv - Biochemistry 2022Quote: ... followed by 10 d of differentiation to dopaminergic neurons (20 ng/ml brain-derived neurotrophic factor, Peprotech; 20 ng/ml glial cell line-derived neurotrophic factor, Peprotech; 1 ng/ml transforming growth factor type β3 ...
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bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in standard 10% RPMI for Jurkat cells and cytokine enriched 10% RPMI (5 ng/ml IL7 and 5 ng/ml IL15, both from Peprotech) for primary T cells for the duration of experiment ...
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bioRxiv - Immunology 2023Quote: ... mouse bone marrow was flushed and cultured for 2 days in stem cell medium (DMEM + 15% FCS + 25ng/ml SCF (PeproTech) + 10ng/ml IL-3 (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...
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bioRxiv - Immunology 2023Quote: CD11b+ mouse lung cells were seeded in 12-well dishes for 2 days in DMEM supplemented with M-Csf (315-02, Peprotech). Cells were stimulated with murine IL-6 for 72 hours ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 1x106 cells were plated per well of 96-well round bottom plate and treated with 10ng/mL of TGF-β1 (Peprotech) or equivalent volume of DMSO for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... Hepatocyte-like cells were made by further incubating the cultures in Stage 3a media (HCM containing 20ng/mL Hepatocyte Growth Factor (Peprotech)) for 5 days followed by a further 15 days in Stage 2b media (HCM containing 10 𝜇g/mL Oncostatin M (R&D Systems Cat No ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Cancer Biology 2023Quote: ... CLL PBMCs were cocultured on irradiated NIH3T3 fibroblasts stably transfected with CD40 ligand or parental cells with or without interleukin-4 (IL-4, 10ng/ml, Peprotech) and IL-21 (25ng/ml ...
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bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Biophysics 2021Quote: ... bone marrow progenitor cells were cultured in complete RPMI media supplemented with interleukin-4 (IL-4, 10 ng/mL, PeproTech, Canada) and granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Developmental Biology 2021Quote: ... and treated with 25 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 24 hours.
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with either low-serum media or low-serum media supplemented with 20 ng/mL HGF (PeproTech, 100-39) or by standard culture media supplemented with Lomustine (Selleckchem ...
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bioRxiv - Neuroscience 2021Quote: ... NSCs were detached as described for cell maintenance and seeded at a density of 100,000 cells into precoated 24 wells in N2B27 medium supplemented with 20 ng/ml BMP4 (Peprotech, 315-27). After 3 days of differentiation in BMP4-supplemented N2B27 medium ...
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bioRxiv - Neuroscience 2021Quote: ... cells were detached with 0.05 % trypsin and re-plated on 0.1 % gelatin-coated flasks as single cells in N2B27 medium supplemented with 20 ng/ml FGF (Peprotech, 450-33) and 20 ng/ml EGF (Peprotech ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
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bioRxiv - Cell Biology 2021Quote: ... PMNs (2.5×106 cells/ml) were used as a control or stimulated with 10 ng/ml TNFα (PeproTech #AF-300-01A) for 15 min at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of TNF in cell culture supernatants was quantified by enzyme-linked immunosorbent assay (ELISA) following the instructions provided by the manufacturer (Peprotech, UK). Absorbance at 405 nm ...
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates in 100 µl media per well at a density of 40,000 cells/ml one day prior to treatment with soluble recombinant TRAIL (rTRAIL; Peprotech-310-04) or MEDI3039 (Medimmune) ...
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bioRxiv - Biochemistry 2022Quote: ... purified cells were cultured in complete medium added with 100 ng/ml macrophage colony-stimulating factor (M-CSF) (Peprotech, Stockholm, Sweden) or 100 ng/ml granulocyte–macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were subject to cytokines stimulation in serum free medium containing 10 ng/ml rat IFN-γ (Peprotech, #400-20) and 10 ng/ml rat TNF-α (Peprotech ...
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Combination image flow cytometry reveals novel methods for isolating megakaryocyte progenitors cellsbioRxiv - Cell Biology 2019Quote: ... 1×107 cells were incubated in IMDM + 10% FBS supplemented with 50 ng/ml of recombinant murine TPO (PeproTech, #315-14) in a T75 flask at 37 °C in 5% CO2 for 72 h.
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bioRxiv - Immunology 2019Quote: ... Lin- bone marrow cells were grown in culture with complete IMDM medium supplemented with 10 ng/ml M-CSF (Peprotech, QC).
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bioRxiv - Immunology 2021Quote: ... then the remaining negatively sorted cells were resuspended at 5×105 cells/ml in complete Iscove’s modified Dulbecco’ s medium supplemented with Granulocyte-macrophage colony-stimulating factor (GM-CSF) (PeproTech, #315-03), FLT-3L (PeproTech ...
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bioRxiv - Immunology 2020Quote: PBMCs were plated in 24-well plates at 2×106 cells per well with 100 IU/ml IL-2 (PeproTech, USA) in the complete medium ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 20 ng/ml glial cell line-derived neurotrophic factor and 20 ng/ml brain-derived neurotrophic factor (Peprotech, East Windsor NJ). Cells were plated on polyornithine/laminin-coated wells ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The cells were collected and cultured in X-Vivo (BE02-060F, Ozyme, France) supplemented with 50 ng/mL M-CSF (Peprotech, France) at a density of 2 x105 cells/mL ...