Labshake search
Citations for Click Chemistry Tools :
151 - 200 of 346 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and biotin-DADPS-azide (8) were purchased from Click Chemistry Tools. Full chemical structures of key reagents are shown in Fig ...
-
bioRxiv - Bioengineering 2023Quote: ... and BTTES were purchased from Click Chemistry Tools. ATTO643 azide was purchased from ATTO-Tech GmbH ...
-
bioRxiv - Cancer Biology 2023Quote: ... Click reaction was performed to conjugate biotin-azide (Cat# 1265, Click Chemistry Tools) to EdU labeled nascent DNA and sonicated (Bioruptor Pico sonication device) ...
-
bioRxiv - Cancer Biology 2023Quote: ... The Click-iT® Alexa Fluor 555 azide reaction was then performed according to the manufacturer’s instructions (Click Chemistry Tools). After washing with 1x PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
-
bioRxiv - Cell Biology 2023Quote: ... EdU detection was performed using an in-house developed Click-iT EdU reaction cocktail made of 200 nM AZDye™ 488 Azide (Cat. No. 1275, Click Chemistry Tools, Scottsdale, AZ), 800 μM Copper (II ...
-
bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
bioRxiv - Bioengineering 2023Quote: ... and 2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl) acetic acid (BTTAA) (catalog no. 1236) were purchased from Click Chemistry Tools. Iodoacetamide and 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride (DMB ...
-
bioRxiv - Biochemistry 2023Quote: Cy3 and Cy5 maleimide were purchased from Click Chemistry Tools. S6-Cy5/L9-Cy3 ribosomes were prepared by partial reconstitution of ΔS6-30S and ΔL9-50S subunits with S6-41Cys-Cy5 and L11-11Cys-Cy3 proteins as previously described [13 ...
-
bioRxiv - Genomics 2023Quote: ... The modified DNA was added to 1µl [1.0mM] Disulfide Biotin DBCO (Click Chemistry Tools A112-5) and incubated at 37°C for 2 hours ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools) was first dissolved at 20mM in DMSO and added to the Fibrinogen solution dropwise to a 0.16mM final concentration ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Methyltetrazine (mTz)-PEG5-NHS Ester (Click Chemistry Tools) was first dissolved at 8.8mM in DMSO and added to GelMA solution dropwise with stirring to 0.88mM final concentration ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Ligands (GFP and mCherry) were modified using trans-Cyclooctene (TCO)- PEG4-NHS Ester (Click Chemistry Tools) to generate GFP-TCO and mCherry-TCO ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
-
bioRxiv - Biochemistry 2023Quote: ... HEPES and Cy5-azide dye (Click Chemistry Tools) were added to the protein sample at a final concentration of 25 mM and 350 µM ...
-
bioRxiv - Biochemistry 2022Quote: For the acid-cleavable reagent (DADPS Biotin Azide, CLICK CHEMISTRY TOOLS) ligated sample ...
-
bioRxiv - Biochemistry 2022Quote: ... Fluorophores for protein labeling were from Click Chemistry Tools Inc.
-
bioRxiv - Bioengineering 2022Quote: ... biotinylated DNAs at 200 μM were also adjusted to pH 8.0 and conjugated with UV cleavable DBCO-NHS ester (Click Chemistry Tools) at 1:20 molar ratio for 2 h ...
-
bioRxiv - Bioengineering 2022Quote: ... 50 μL antibodies were then modified with UV-cleavable azido-NHS ester (Click Chemistry Tools) at 1:15 molar ratio for 2 h ...
-
bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
-
bioRxiv - Bioengineering 2022Quote: ... 30 μL of 200 μM amine-ended biotinylated oligos was incubated with 100 mM photocleavable DBCO-NHS ester (Click Chemistry Tools) at 1 ...
-
bioRxiv - Bioengineering 2022Quote: ... were modified with heterobifunctional Sulfo-DBCO Maleimide linker (Click Chemistry Tools) to arm the peptide with an azide-reactive group (dibenzocyclooctyne group ...
-
bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... 100 uM Biotin-Azide (1167-5, Click Chemistry Tools) and incubated for another 1 h ...
-
bioRxiv - Biochemistry 2022Quote: ... and TCO-PEG4-NHS ester (Click Chemistry Tools), respectively.
-
bioRxiv - Biochemistry 2022Quote: ... Stock solutions of biotin-tetrazine (biotin-PEG4-methyltetrazine, Click Chemistry Tools), bis-biotinamide (bis-dPEG3-biotin ...
-
bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
-
bioRxiv - Genomics 2022Quote: ... 10 µL of 10 mM DBCO-NHS (Click Chemistry Tools cat# A134-10) was then added to the antibodies and allowed to rotate at room temperature for one hour 26 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2 mM THPTA (Click Chemistry Tools 1010-100), and 100 µM copper sulfate (Fisher Scientific AC197722500) ...
-
bioRxiv - Cell Biology 2022Quote: ... alkynyl-palmitate (Click Chemistry Tools; Scottsdale, AZ), Cy5.5 (tetramethylindo)(di)- carbocyanines ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were then washed with ice-cold D-PBS 4 times and incubated in D-PBS supplemented with 1 μM Cy5.5-azide (Click Chemistry Tools), 1 mM TCEP (adjusted to pH 7.0) ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected with pcDNA3.1-SARS CoV-2-Spike-C9 and incubated overnight in DMEM supplemented with 10% (v/v) charcoal-stripped FBS with or without 100 μM of clickable fatty acid analogs (Click Chemistry Tools, Scottsdale, AZ) coupled to albumin ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...
-
bioRxiv - Neuroscience 2022Quote: 1M Azidohomoalanine (AHA) stock solution was made from powder (Click Chemistry Tools, Cat# 1066100) in 1xPBS with pH adjusted to 7 using 1N NaOH ...
-
bioRxiv - Neuroscience 2022Quote: ... 5 mM Biotin-PEG4-Alkyne (Click Chemistry Tools, Cat#TA105100) and 50 mM TCEP (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... and AF488 azide (0.5 μM resuspended in DMSO, Click Chemistry Tools 1275) for 30 min at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
-
bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
-
bioRxiv - Biophysics 2022Quote: ... Stock solutions were made as follows: Cy3 and Cy5 alkyne dyes (Click Chemistry Tools) 10 mM in DMSO ...
-
bioRxiv - Biophysics 2022Quote: ... BTTES (Click Chemistry Tools) 50 mM ...
-
bioRxiv - Bioengineering 2022Quote: ... Dibenzocyclooctyne acid was purchased from Click Chemistry Tools (Scottsdale, AZ). Cytochalasin D was obtained from Santa Cruz Biotech ...
-
bioRxiv - Cell Biology 2022Quote: ... EdU was labelled using the 647 azide plus kit (Click-&-Go Plus EdU 647, Cat#1353, Click Chemistry Tools) according to the manufacturers protocol ...
-
bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
-
bioRxiv - Cancer Biology 2022Quote: ... Biotin-alkyne (#1266-5) and TAMRA-alkyne (#1255-5) were purchased from Click Chemistry Tools (Scottsdale, AZ). O-GlcNAc Chemoenzymatic labeling using the Click-IT™ kit were carried out as recommended by the manufacturer (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2022Quote: ... TCO-NHS ester (Click Chemistry Tools, USA), and p-SCN-Bn-deferoxamine (Macrocyclics ...
-
bioRxiv - Cancer Biology 2022Quote: ... tetrazine-PEG5-NHS ester (Click Chemistry Tools, USA), TCO-NHS ester (Click Chemistry Tools ...
-
bioRxiv - Genetics 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...