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Citations for Click Chemistry Tools :
1 - 50 of 314 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... and NHS-PEG6-Alkyne) were purchased from Click Chemistry Tools. Fmoc- N-amido-dPEG12-TFP ester was purchased from Quanta BioDesign ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... elegans eggs were hatched into L1 larvae for 24 h without food in M9 buffer containing 1 mM tetra-acylated azidoacetylgalactosamine (GalNAz, Click Chemistry Tools) or M9 buffer containing equal volume of the vehicle (DMSO) ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with 5 μM picolyl azide dye (Click Chemistry Tools, Scottsdale, AZ) for 10 minutes at room temperature.
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bioRxiv - Cell Biology 2024Quote: AF488 picolyl azide and Dde biotin picolyl azide were purchased from Click Chemistry Tools (Scottsdale, AZ). DNA Isolation Midi-Prep Plus and DNA Clean and Concentrator Kits were purchased from Zymo Research ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5-ethynyldeoxyuridine (EdU, Sigma-Aldrich or Click Chemistry Tools) was at 10mM in DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labeled with 20μM EdU (Click Chemistry Tools) for 20 min and EdU was clicked to a mixture of Alexa488 and biotin azides at molar ratio of 1 to 50 ...
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bioRxiv - Neuroscience 2024Quote: ... were submerged in dimethyl sulfoxide (DMSO) with 10 mM dibenzocyclooctyne-PEG-amine (DBCO-PEG4-amine; Click Chemistry Tools, A103P) overnight at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... or Fluorescein Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) and a premixture of 100 μM tris((1-benzyl-1H-1,2,3-triazol-4-yl ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Neuroscience 2024Quote: ... L-Azidohomoalanine (AHA) (Click Chemistry Tools 1066) was used at 4 mM in R/L-aCSF (AHA-aCSF ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
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bioRxiv - Cell Biology 2024Quote: ... BTTAA (Click chemistry tools, 1236-100); CalFluor 647 Azide(Click chemistry tools ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Bioengineering 2024Quote: ... and were used for conjugation with dibenzocyclooctyne (DBCO)-N-hydroxysuccinimidyl (NHS) ester (#A133, Click Chemistry Tools). Typically ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... was conjugated to 0.3 mM azidoacetic acid NHS ester (#1070, Click Chemistry Tools) in a 300 μl PBS ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to block any unreacted BCN groups ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to label the SPAAC network and then washed 3 x 1 hr in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... dicysteines resuspended at a 40 mM concentration were prereacted with 100 µM of AlexaFluor 488 maleimide (1:400 dye:peptide) (Click Chemistry Tools; Scottsdale, AZ) for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... whereas PEG-BCN was preincubated for 15 minutes with 50 µM of AlexaFluor 568 azide (1:200 dye:PEG) (Click Chemistry Tools; Scottsdale, AZ). DN hydrogels were then formed with these fluorescently tagged components ...
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bioRxiv - Bioengineering 2024Quote: ... and then incubated with 50 µM AZDye 488-tetrazine (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to visualize the thiol-ene network ...
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bioRxiv - Biochemistry 2024Quote: ... Site-specific labeling with Cy3 and Cy5 fluorophores was done by diluting Cy3 and Cy5 alkyne dyes (Click Chemistry Tools) to the final concentration of 40 µM each in the extracellular buffer and incubating for 15 minutes in the incubator ...
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bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
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bioRxiv - Biophysics 2024Quote: ... AFDye 488 NHS Ester (Click Chemistry Tools) was added to FUS at a dye:protein molar ratio of 20:1 and incubated in dark at room temperature for 30 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mg/l SILAC-heavy L-arginine (Silantes #201604102) and 0.1 mM L-azidohomoalanine (Click Chemistry Tools #1066-1000). The other half of the cells were labelled in AHA-SILAC-light medium containing regular L-lysine ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mg/l SILAC-heavy L-arginine (Silantes #201604102) and 0.1 mM L-azidohomoalanine (Click Chemistry Tools #1066-1000), while the other half of the cells were subjected to AHA-SILAC-light medium containing regular L-lysine ...
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bioRxiv - Cancer Biology 2024Quote: ... oligonucleotides were derivatized with TCO-PEG4-NHS (Click chemistry tools) in 10x borate buffered saline (BBS) ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were conjugated to mTz-PEG4-NHS (Click chemistry tools) at first in 1x BBS ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Genomics 2024Quote: ... we performed a click reaction between the azide-linked modifications (products from the previous reaction) and the strained alkyne DBCO-PEG4-biotin (Click Chemistry Tools). The resulting biotinylated-5hmC-modified cfDNA fragments were then affinity captured using Dynabeads M-270 Streptavidin (Thermo) ...
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bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
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bioRxiv - Cell Biology 2024Quote: ... aggregates were embedded as described above and cultured in CK+DCI media with 50 µM azide-modified galactosamine (Click Chemistry Tools Catalog #2281). Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5 ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Biophysics 2024Quote: ... was incubated on a rocker with a 1.5-fold molar excess (15 μM) of dibenzocyclooctyne-amine fluorophore (DBCO-MB543, Click Chemistry Tools Inc.) for 2 h at 4 °C ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Molecular Biology 2023Quote: ... Stocks of N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz, Click Chemistry Tools) were made to 500 mM in sterile dimethyl sulfoxide (DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.
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bioRxiv - Physiology 2023Quote: ... and 1.14 μl Biotin-N3 (stock solution: 9.67 mM in DMSO; 1265, Click Chemistry Tools, Scottsdale, AZ), were added for each reaction and this was incubated at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2023Quote: ... or Fluorescein Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) and a premixture of 100 μM tris((1-benzyl-1H-1,2,3-triazol-4-yl ...
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bioRxiv - Biophysics 2023Quote: ... for further conjugation with DBCO-PEG4-NHS (Click Chemistry Tools, A134-2) via succinimidyl ester chemistry ...
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bioRxiv - Neuroscience 2023Quote: ... Alkylated and cleaned sample of AHA/ANL incorporated samples were clicked to a disulfide-biotin alkyne (DST) (Click Chemistry Tools, #1498) as described before ...
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bioRxiv - Molecular Biology 2023Quote: ... is incubated with DBCO-Peg5-NHS Ester (linker; 10mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (10 μg of antibody and 2.8 μg of linker ...