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Citations for Click Chemistry Tools :
1 - 50 of 346 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... or Fluorescein Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) and a premixture of 100 μM tris((1-benzyl-1H-1,2,3-triazol-4-yl ...
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bioRxiv - Cell Biology 2024Quote: AF488 picolyl azide and Dde biotin picolyl azide were purchased from Click Chemistry Tools (Scottsdale, AZ). DNA Isolation Midi-Prep Plus and DNA Clean and Concentrator Kits were purchased from Zymo Research ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5-ethynyldeoxyuridine (EdU, Sigma-Aldrich or Click Chemistry Tools) was at 10mM in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... BTTAA (Click chemistry tools, 1236-100); CalFluor 647 Azide(Click chemistry tools ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were labeled with 20μM EdU (Click Chemistry Tools) for 20 min and EdU was clicked to a mixture of Alexa488 and biotin azides at molar ratio of 1 to 50 ...
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bioRxiv - Neuroscience 2024Quote: ... L-Azidohomoalanine (AHA) (Click Chemistry Tools 1066) was used at 4 mM in R/L-aCSF (AHA-aCSF ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Cell Biology 2024Quote: ... aggregates were embedded as described above and cultured in CK+DCI media with 50 µM azide-modified galactosamine (Click Chemistry Tools Catalog #2281). Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5 ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Biophysics 2024Quote: ... was incubated on a rocker with a 1.5-fold molar excess (15 μM) of dibenzocyclooctyne-amine fluorophore (DBCO-MB543, Click Chemistry Tools Inc.) for 2 h at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...
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bioRxiv - Genomics 2024Quote: ... we performed a click reaction between the azide-linked modifications (products from the previous reaction) and the strained alkyne DBCO-PEG4-biotin (Click Chemistry Tools). The resulting biotinylated-5hmC-modified cfDNA fragments were then affinity captured using Dynabeads M-270 Streptavidin (Thermo) ...
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bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
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bioRxiv - Biochemistry 2024Quote: ... Site-specific labeling with Cy3 and Cy5 fluorophores was done by diluting Cy3 and Cy5 alkyne dyes (Click Chemistry Tools) to the final concentration of 40 µM each in the extracellular buffer and incubating for 15 minutes in the incubator ...
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bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mg/l SILAC-heavy L-arginine (Silantes #201604102) and 0.1 mM L-azidohomoalanine (Click Chemistry Tools #1066-1000). The other half of the cells were labelled in AHA-SILAC-light medium containing regular L-lysine ...
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bioRxiv - Biochemistry 2024Quote: ... 40 mg/l SILAC-heavy L-arginine (Silantes #201604102) and 0.1 mM L-azidohomoalanine (Click Chemistry Tools #1066-1000), while the other half of the cells were subjected to AHA-SILAC-light medium containing regular L-lysine ...
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bioRxiv - Bioengineering 2024Quote: ... and were used for conjugation with dibenzocyclooctyne (DBCO)-N-hydroxysuccinimidyl (NHS) ester (#A133, Click Chemistry Tools). Typically ...
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bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... was conjugated to 0.3 mM azidoacetic acid NHS ester (#1070, Click Chemistry Tools) in a 300 μl PBS ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to block any unreacted BCN groups ...
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bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to label the SPAAC network and then washed 3 x 1 hr in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... dicysteines resuspended at a 40 mM concentration were prereacted with 100 µM of AlexaFluor 488 maleimide (1:400 dye:peptide) (Click Chemistry Tools; Scottsdale, AZ) for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... whereas PEG-BCN was preincubated for 15 minutes with 50 µM of AlexaFluor 568 azide (1:200 dye:PEG) (Click Chemistry Tools; Scottsdale, AZ). DN hydrogels were then formed with these fluorescently tagged components ...
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bioRxiv - Bioengineering 2024Quote: ... and then incubated with 50 µM AZDye 488-tetrazine (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to visualize the thiol-ene network ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were conjugated to mTz-PEG4-NHS (Click chemistry tools) at first in 1x BBS ...
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bioRxiv - Cancer Biology 2024Quote: ... oligonucleotides were derivatized with TCO-PEG4-NHS (Click chemistry tools) in 10x borate buffered saline (BBS) ...
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bioRxiv - Biophysics 2024Quote: ... AFDye 488 NHS Ester (Click Chemistry Tools) was added to FUS at a dye:protein molar ratio of 20:1 and incubated in dark at room temperature for 30 minutes ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... and NHS-PEG6-Alkyne) were purchased from Click Chemistry Tools. Fmoc- N-amido-dPEG12-TFP ester was purchased from Quanta BioDesign ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... elegans eggs were hatched into L1 larvae for 24 h without food in M9 buffer containing 1 mM tetra-acylated azidoacetylgalactosamine (GalNAz, Click Chemistry Tools) or M9 buffer containing equal volume of the vehicle (DMSO) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1 × 10−4 M AHA (1066-25, Click Chemistry Tools), 4 × 10−3 M GlutaMAX supplement (35050061 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores were purchased from Click Chemistry Tools or Fluoroprobes ...
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bioRxiv - Biochemistry 2024Quote: ... Cy5-azide (Click Chemistry Tools AZ118-1) was linked to 17-ODYA ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4- yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools >95%), sodium DL-lactate (NaC3H5O3 ...
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bioRxiv - Bioengineering 2024Quote: CalFluor 488 (Click Chemistry Tools), alkyne-PEG4-acid (Click Chemistry Tools) ...
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bioRxiv - Bioengineering 2024Quote: ... alkyne-PEG4-acid (Click Chemistry Tools), copper(II ...
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bioRxiv - Cell Biology 2024Quote: ... Ac4GalNAz and Ac4GlcNAz (Click Chemistry Tools) were resuspended in DMSO to stock concentration of 100 mM ...
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bioRxiv - Neuroscience 2024Quote: ... Azidohomoalanine (AHA) (Click Chemistry Tools, #1066-100) was dissolved in ddH2O at a stock concentration of 100 mM and diluted to 1 mM in sterile saline ...
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bioRxiv - Neuroscience 2024Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools, #1309-1), CuSO4 and additive overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... azide fluorophore (AZDye 405 Azide, Click Chemistry Tools 1307-5) to a concentration of 30 μM ...
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bioRxiv - Bioengineering 2024Quote: ... Media was supplemented with a mixture of L-homoproparglyglycine (HPG, Click chemistry tools 354232) and methionine (Millipore Sigma M5308 ...
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bioRxiv - Biochemistry 2024Quote: ... The eluted protein was mixed with DDM to a final concentration of 0.1% and mixed with a ten-fold excess of Cy3 Maleimide (Click Chemistry Tools, stock dissolved in DMSO). The labeling reaction proceeded for 2 hours at room temperature ...