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Citations for Cell Signaling Technology :
151 - 196 of 196 citations for Recombinant Rat Il6st His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The rabbit anti-rat GSDMD (L60) (#93709) were bought from Cell Signaling Technology (CST, United States). We obtained Goat Anti-rabbit IgG/FITC (bs-0295G-FITC ...
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bioRxiv - Immunology 2023Quote: ... antibodies followed by HRP-conjugated secondary antibodies (anti-Rabbit or anti-Rat antibodies (Cell Signaling Technology).
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: HRP-conjugated anti-rat (Goat, Cell Signaling #7077, 1:5000), or HRP-conjugated anti-rabbit (Goat ...
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Neuroscience 2019Quote: ... Rat brain sections were incubated with following antibodies: rabbit anti-c-Fos (1:1000) (Cell Signaling, 2250S), Mouse anti-CamKII (Abcam ...
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bioRxiv - Immunology 2020Quote: ... then incubated with horseradish peroxidase-linked anti-rat secondary antibody (1:3000, Cell Signaling Technology®, 7077S) or anti-mouse secondary antibody (1:3000 ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-linked anti-mouse IgG and HRP-linked anti-rat IgG from Cell Signaling Technology (Danvers, MA) were used as secondary antibodies.
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bioRxiv - Biochemistry 2021Quote: ... HRP-linked anti-mouse IgG and HRP-linked anti-rat IgG from Cell Signaling Technology (Danvers, MA) were used as secondary antibodies.
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bioRxiv - Cancer Biology 2022Quote: ... HRP-linked anti-mouse IgG and HRP-linked anti-rat IgG from Cell Signaling Technology (Danvers, MA) were used as secondary antibodies.
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were detected using the secondary anti-rat IgG HRp-linked (Cell Signaling, 7077S, 1:1000) together with the SuperSignal™ West Femto Chemiluminescent Substrate (Thermo Fischer Scientific).
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with a 1:500 dilution of Alexa Fluor® 488-labeled rabbit anti-His tag antibody (Cell Signaling Technology, Danvers, MA) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibody anti-rat IgG HRP (catalog# 7077) was bought from Cell Signaling Technology (CST, Danvers, MA). All other reagents and chemicals ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers ...
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bioRxiv - Neuroscience 2024Quote: ... Merck NE1019, RRID:AB_604550; rat anti-MBP 1:500, Abcam ab7349, RRID:AB_305869; rabbit anti-FLAG 1:500, Cell Signaling Technology 14793 ...
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bioRxiv - Developmental Biology 2022Quote: ... rat TS cells were exposed to a phosphatidylinositol 3-kinase (PI3K) inhibitor (LY294002, 10 μM, 9901, Cell Signaling Technology), chloroquine (50 μM for 24 h ...
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bioRxiv - Immunology 2023Quote: ... CD208/DC-LAMP rat monoclonal antibody (eurobio #Cat.DDX0192P-100) (1/100) and Cathepsin B rabbit antibody (Cell Signaling Cat.D1C7Y)(1/300 ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076 ...
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bioRxiv - Bioengineering 2020Quote: ... followed by incubation with 1:2,500 dilution of primary antibodies for CD68 (rat anti-mouse, ABD SeroTec) and cleaved Casp3 (rabbit anti-mouse, Cell Signaling) in 0.5% w/v BSA in 1X PBS for 24 h at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... isolated rat cardiomyocytes were lysed in RIPA buffer (Cayman #10010263) supplemented with protease and phosphatase Inhibitor cocktail (Cell Signaling #5872S) on ice for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... which were detected with HRP-conjugated goat anti-rat immunoglobulin-G at 1:5000 dilution (Cell Signaling Technology, Cat# 7077S) or goat anti-rabbit immunoglobulin-G at 1:3000 dilution (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit monoclonal anti-CREB1 1:2,000 diluted in the corresponding blocking solution (5% BSA-TBST) (Cat# 9197, RRID: AB_331277, immunogen: recombinant protein specific to the amino terminus of human CREB1 protein, Cell Signaling Technology, Danvers, MA, USA); rabbit affinity isolated anti-actin 1:3,500 diluted in PBST (Cat# A2066 ...
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bioRxiv - Neuroscience 2023Quote: ... penicillin/streptomycin and 2% Fetal bovine serum (for vehicle group) or the same media with 100ng/ml recombinant colony-stimulating factor 1 (Csf1, Cell Signaling, Boston, MA, USA). Csf1 stock was diluted in saline.
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation in rat-anti-MAC2 antibody (Biozol, Eching, Germany; 1:100 diluted in Signalstain, Cell Signaling, Danvers, MA, USA) was conducted overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... HRP-linked anti-mouse IgG or HRP-linked anti-rat IgG were used as secondary antibodies (Cell Signaling Technology, Danvers, MA). Membranes were imaged with ChemiDoc imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... and further incubated with the secondary antibody (1:5,000 anti-rat [7077S]; anti-rabbit [7074S]; anti-mouse [7076S] (Cell Signaling Technology)) conjugated to horseradish peroxidase followed by ECL (Thermo Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam ...
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (Cell Signaling Technology, #3195, 1:100 dilution), anti-Myosin-VIIa rabbit polyclonal antibody (Proteus BioSciences Inc. ...
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bioRxiv - Developmental Biology 2020Quote: The following primary and secondary antibodies were used for immunofluorescence: anti-E-cadherin rat antibody (#3195, 1:100 dilution, Cell Signaling Technology), anti-SOX9 rabbit antibody (#AB5535-25UG ...
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bioRxiv - Neuroscience 2021Quote: Antibodies on these sections included Rat anti-CD3 epsilon (1:100, NBP1-26685, Novus Bio) and Rabbit anti-C-Kit (1:100, D13A2, Cell Signaling Tech).
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bioRxiv - Molecular Biology 2020Quote: ... After 3 washing steps (5 min each) with PBS-T membranes were incubated with horseradish-linked goat anti-rat (1:2000 dilution; 7077S; Cell Signaling Technology) or goat anti-rabbit (1:2000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500, rat anti-Flag 1:500 Novus NBP1-06712, mouse anti-NeuN Ab 1:500, Cell Signaling #94403S). For secondary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were then washed in 1X TBS with 0.05% Tween 20 and incubated with HRP-linked anti-rat secondary antibody (1:3000, Cell Signaling Technology®, 7077S) or anti-mouse secondary antibody (1:3000 ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat): anti-Pan-AKT (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-rat secondary antibody was used for detection of primary antibodies and visualized by SignalStain DAB Substrate kit (Cell Signaling Technology, Danvers, MA). All stained tissues were blindly evaluated by pathologists ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-rat IgG secondary antibody (Cell Signaling Technology, Danvers, MA, USA) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... mouse anti-MSP1 antibody (European Malaria Reagent Repository, 1:500), rat anti-PfBiP MRA-1247 (BEI Resources, NIAID, NIH, 1:100) and mouse anti-HA(6E2) (Cell Signaling Technology Inc., 1:100). Anti-mouse and anti-rat antibody conjugated to Alexa Fluor 488 or Alexa Fluor 546 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:100), Rat anti-GFP monoclonal antibody (nacalai tesque, GF090R, diluted 1:1000) and rabbit anti-pSmad1/5/9 antibody (Cell Signaling Technology, #13820, diluted 1:100). After the primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...