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Citations for Cell Signaling Technology :
101 - 150 of 196 citations for Recombinant Rat Il6st His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... followed by detection with 1μg/mL of AF647-conjugated secondary anti-HIS antibodies (Cell Signaling, Danvers, MA) for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was isolated by immunoprecipitation using a mouse anti-His antibody (Cell Signaling, catalog #: 2366S) followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti-His tag antibody (catalog #: 2366S) was obtained from Cell Signaling Technology (Danvers, MA).
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bioRxiv - Neuroscience 2022Quote: ... or anti-rat antibody (1: 4,000, #7077, RRID: AB_10694715, Cell Signaling, USA) for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat IgG HRP-linked antibody (1:10000, 7077, Cell Signaling Technologies). Immunoblot blot were developed using the Clarify™ Western ECL substrate (BioRad ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-rat IgG HRP-linked antibody (Cell Signaling Technology, 1:3000). All p53 constructs were cloned into pcDNA3.1 ...
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bioRxiv - Microbiology 2023Quote: ... and anti-rat IgG (Cell Signaling Technology, [7076S, 7074S, 7077S], 1:10,000). All blots were incubated with either SuperSignal West Pico PLUS ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight in)and probed again with fluorescent-tagged goat IgG to rabbit IgG (1:500, Cell Signaling Technology; room temperature, 1 h), both in PBS supplemented with 1% BSA and 0.3% Triton X-100 ...
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bioRxiv - Microbiology 2023Quote: ... HA- and EGFP-tagged proteins were incubated with anti-HA antibody (1: 2,000; 2999, Cell Signaling Technology, Danvers, MA, USA) or anti-EGFP antibody (1 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with Alexa Fluor 488 or Alexa Fluor 555 tagged anti-mouse antibody (1:1000, Cell Signaling Technologies) for 1hr at RT in a humid black chamber ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then incubated with the recombinant pAG-MNase (Cell Signaling, 40366; 1:33 volume) in 150 µl Perm buffer per well for an hour at room temperature ...
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bioRxiv - Systems Biology 2024Quote: ... U-2 OS cells underwent treatment with 50 ng/mL recombinant IFN-γ (Cell Signaling). The cytokine was diluted in 0.5% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was run on an SDS PAGE and probed with anti-his antibody (Cat # 2365, Cell Signaling Technologies).
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Bioengineering 2019Quote: ... and stained following standard protocols previously described (Xue et al. 2011) with primary antibodies against 3xHA-tagged Nme2Cas9 (anti-HA; Cell Signaling) and mCherry (anti-RFP ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged and myc-tagged proteins were detected with rabbit anti-DYKDDDDK (FLAG) monoclonal antibody and rabbit anti-myc monoclonal antibody (Cell signaling), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-rat horseradish peroxidase–conjugated secondary antibodies (1:1000, Cell Signaling Technology). The signal was detected using SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were then probed with secondary antibodies anti-rat IgG (7077S; Cell Signaling), anti-mouse IgG (7076S ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-rat Cyto c monoclonal antibody (1:1000, Cell Signaling Technology, USA), rabbit anti-rat Cox-IV monoclonal antibody (1:2000 ...
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bioRxiv - Immunology 2020Quote: ... Proteins were visualized by staining with anti-rat (1:3000, 7077, Cell Signaling) or anti-mouse (1:3000 ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-rat horseradish peroxidase–conjugated secondary antibodies (1:1000, Cell Signaling Technology). The signal was detected using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... and HRP-conjugated goat anti-rat IgG (1:5,000, 7077, Cell Signaling Technology).
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bioRxiv - Synthetic Biology 2023Quote: ... and CD45 (30-F11) Rat mAb (cat. no. 55307S, Cell Signaling, Danvers, MA). The antibodies were diluted 1:200 in 5% goat serum and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... rat anti-Emp and rabbit anti-α-tubulin (1:2000, Cell Signaling, 11H10). For western blot analysis ...
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bioRxiv - Immunology 2024Quote: ... membranes were incubated with HRP-conjugated anti-rat IgG (Cat# 7077S; Cell Signaling) for CASP-1 or HRP-conjugated anti-heavy chain of rabbit IgG (Cat# HRP-66467 ...
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bioRxiv - Molecular Biology 2023Quote: ... and myc- and HA-tagged proteins were immunoprecipitated with Protein A/G beads (GeneBio, 22202B-1) coated with anti-myc antibody (Cell signaling, 2276S) at a dilution of 1:250 or anti-HA antibody (Cell signaling ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were sorted for the coexpression of each component via fluorescence-activated cell sorting on a FACS ARIA II (Beckton-Dickinson) by staining with appropriate fluorescently tagged anti-Myc and anti-Flag antibody for 30 minutes at 4°C (Cell Signaling Technologies) or expression of the transgenes ...
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bioRxiv - Plant Biology 2020Quote: ... Secondary ∝-rat-HRP was used at a dilution of 1/10,000 (Cell Signaling Technology).
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bioRxiv - Biochemistry 2021Quote: ... Adult rat mitochondrial protein was added for comparative control and α-tubulin (Cell Signaling) was used as the loading control ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used were goat anti-Rat-IgG (Cell Signaling, 7077s, 1:1000) and goat anti-mouse (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... the top five recombinant p-S65-Ub antibodies or the reference antibody (Cell Signaling Technology, 62802, E2J6T) were used as capturing agents ...
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bioRxiv - Molecular Biology 2022Quote: Synchronized young adult animals expressing N-terminal HA-tagged PMK-1 were used for immunoprecipitation with anti-HA-Tag (C29F4) rabbit mAb (Cell Signaling Technology 11846) and eluted with 2x laemmli buffer ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in 5% milk in PBST for 1 hour at room temperature and incubated overnight at 4°C with agitation in primary antibody at the following dilutions: rabbit anti-His (1:1,000, Cell Signaling, 2365), rabbit anti-GST (1:1,000 ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... 1 μg/ml of recombinant antibodies and 0.2 μg/ml of the reference antibody (Cell Signaling Technology, 62802) were used ...
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bioRxiv - Physiology 2019Quote: ... Anti-rat or anti-rabbit IgG conjugated to HRP (Cell Signaling Technology Danvers, MA, USA) were used as secondary antibody ...
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bioRxiv - Physiology 2022Quote: ... or goat anti-rat IgG HRP-conjugated (1:2000, catalog no. 7077, Cell Signaling Technology). Membranes were then washed with TBS + 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2023Quote: ... secondary antibody was added (anti-rat Cell Signaling 7077S, 1:5000, anti-mouse Cell Signaling, 7076S ...
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bioRxiv - Genomics 2023Quote: ... The presence of the correctly tagged MYC protein was validated by Western blot probed with a c-MYC antibody (Rabbit mAb, Cell Signaling Technology, Cat# 5605). The recombinant pAAV-CRE plasmid was packaged in HEK293T cells using pHelper and pRC2-mi342 plasmids (Takara ...
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bioRxiv - Microbiology 2023Quote: ... The spike protein and fragments were detected by rabbit monoclonal antibodies (mAb) against the 6-His tag (12698S) from Cell Signaling Technology. The nitrocellulose (NC ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... rabbit anti-GATA1 (NF that was produced against recombinant N-terminal zinc finger76, rabbit anti-GAPDH (14C10, Cell Signaling) and anti-β3 integrin (sc-14009 ...
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bioRxiv - Developmental Biology 2020Quote: ... They were probed with rabbit IgG to rat YAP (1:100, Cell Signaling Technology, Boston, USA)and β-catenin (1:300 ...
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bioRxiv - Cancer Biology 2019Quote: ... goat anti-rat IgG conjugated to Alexa Fluor 488 (Cell Signaling Technology, 4416S, 1/1000 dilution) and goat anti-rabbit IgG antibody conjugated to Alexa Fluor 546 (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse anti-human/rat CD44 (5640S) and rabbit anti-human/mouse ALDH1A1 (12035S) (Cell Signaling Technology), rabbit anti-human/mouse CD36 (ab133625 ...
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bioRxiv - Developmental Biology 2022Quote: The followings were used: rat monoclonal anti-E-cadherin (Cell Signaling Technology, #3195, 1:100 dilution), rabbit polyclonal anti-phospho-myosin light chain (pMLC ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse anti-human/rat CD44 (5640S) and HRP-linked anti-rabbit IgG (7074) (Cell Signaling Technology), CD45 monoclonal Antibody (14-9457-80) ...