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Citations for Cell Signaling Technology :
601 - 650 of 768 citations for Protein Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... — 10 mg of digested proteins were dissolved in 1X immunoprecipitation buffer (50 mM MOPS, 10 mM Na2HPO4, 50 mM NaCl, pH 7.2, Cell Signaling). Modified symmetric dimethyl arginine peptides ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10 µg of the protein extracts were resolved by SDS-PAGE and probed using antibodies against STAT3 (1:1,000, #9139S; Cell Signaling), Phospho-STAT3 (1:2,000 ...
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bioRxiv - Neuroscience 2022Quote: ... The antibodies used on membranes containing cytosolic and nuclear protein fractions were: rabbit anti PCBP1 (1:1000, #8534, Cell Signaling), mouse anti PCNA (1:1000 ...
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used in the indicated dilutions: ERK1/2 (#4695, RRID:AB_390779, Cell Signaling Technology, 1:50), JUN (#9165 ...
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bioRxiv - Plant Biology 2019Quote: ... Detection of phosphorylated proteins was performed in blocking solution (5% BSA, 0.1% [v/v] Tween-20) with anti-phosphothreonine antibodies (9381, Cell Signaling) and with anti-rabbit-HRP (W4011 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Plant Biology 2021Quote: ... 6xHis-tagged ALI1 and 6xHis-tagged control protein were incubated with streptavidin beads (Sepharose® Bead Conjugate, Cell Signaling Technology) that were already loaded with M4 (oxo-C14-HSL tagged with biotin ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total p38 mitogen-activated protein kinase antibody (p/total p38 1:1000 Cell Signaling Technology, Danvers, MA). After incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred onto PVDF membranes and probed with the following antibodies: HIF-1α (D2U3T) (#14179, Cell Signaling Technology), BiP (C50B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... a rabbit mAb raised against synthetic peptide corresponding to residues surrounding Ser518 of human Merlin protein from Cell Signaling Technology Cat # 13281 and a mouse monoclonal antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Rabbit anti β-actin antibody at 1:1000 dilution was employed for normalization of total protein amount (8457, Cell Signaling). For revelation ...
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bioRxiv - Plant Biology 2022Quote: ... and probed with anti-phospho-p44/42 mitogen-activated protein kinase (Erk1/2) (Thr202/Tyr204) (Cell Signaling; 1:1,000 dilution), anti-MBP (New England Biolab ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates of equal protein concentration were incubated overnight in 4 °C with an anti-Src antibody (Cell Signaling Technology, #2108). Complexes were pulled out using protein-A sepharose beads (GE-Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... manufacturer and catalog number shown in brackets): Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E, Alexa Fluor 555, 1:200, Cell Signaling, 39855); S6 Ribosomal Protein (54D2 ...
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bioRxiv - Genetics 2023Quote: ... Protein was extracted from frozen tissue by homogenization of tissue with RIPA lysis buffer and PMSF (Cell Signaling, Danvers, MA). Protein concentrations of samples were determined using Bradford Assay (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... HA- and EGFP-tagged proteins were incubated with anti-HA antibody (1: 2,000; 2999, Cell Signaling Technology, Danvers, MA, USA) or anti-EGFP antibody (1 ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... Chromatin complexes were immunoprecipitated with primary antibody as below and incubated with protein G magnetic beads (Cell Signaling Technology #9006) overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and a rabbit antibody recognizing a cellular marker protein (anti-TGN46, Abcam, ab50595, 1:600; anti-EEA1, Cell Signaling Technology, 2411 ...
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bioRxiv - Biochemistry 2023Quote: ... and blots (20-50 µg of total protein) were probed with the following antibodies: HA (3724S, Cell Signaling; 1:1500), BRCA1 (SC6954 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell extract were analyzed after migration of 50µg protein extract by Western blotting using a primary antibody against HES1 (#11988, Cell Signaling). Signals were revealed using a rabbit peroxidase-conjugated secondary antibody (1/5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Up to 30 µg of protein lysate was separated by SDS-PAGE and transferred to nitrocellulose membranes before antibody incubation (IκBα: Cell Signaling, CS9242 ...
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bioRxiv - Developmental Biology 2023Quote: ... the following antibodies were used: rabbit anti-CCAAT/enhancer binding protein alpha (C/EBPA, 1:500, 8178P, Cell Signaling Technology), chicken anti-green fluorescent protein (GFP ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were subjected to enrichment of tyrosine phosphorylated proteins using an the PTMScan HS phopshotyrosine kit (Cell Signaling Technology, #38572) following in-solution tryptic digestion and desalting ...
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bioRxiv - Biochemistry 2023Quote: ... 53BP1FFR proteins (WT and 53BP1-PN) and γH2A.X were stained respectively with HA-Tag (C29F4) rabbit antibody (Cell Signaling Technology) and anti-ψH2A.X (Ser139 ...
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bioRxiv - Cancer Biology 2023Quote: ... A horseradish peroxidase-conjugated β-actin antibody was used to detect this protein loading control (clone 8H10D10, #12262 Cell Signaling).
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-S6 ribosomal protein (Ser240/244) (pS6) (rabbit polyclonal, #2215, RRID: AB_331682, Cell Signaling Technology; 1:1000 for WB), anti-S6 ribosomal protein (S6 ...
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 µg total protein was resolved in 8% non-reducing SDS-PAGE gel and blot with PKM2 antibody (Cell Signaling, 3198s).
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were blocked with Q-PBS and probed with antibodies against the following proteins: p-EGFR Y1068 (#3777S, 1:200, Cell Signaling), MET (#AF276 ...
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bioRxiv - Genetics 2021Quote: ... the samples were incubated with 30 ml of ChIP Grade Protein G magnetic beads (Cat# 9006S; Cell Signaling Technology; Danvers, MA) for 2 hours at 4°C under gentle rotation ...
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bioRxiv - Plant Biology 2022Quote: ... The prey and bait proteins were detected using anti-His (1:1000, No. #2365; Cell Signaling Technology [CST], Danvers, MA, USA) and anti-GST (1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Expression of Anti-HA fusion protein was examined by staining cells with rabbit Anti-HA (1:250; #3724, Cell Signaling Technology) and Alexa Fluor@ 488 donkey anti-rabbit IgG (1:2000 ...
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bioRxiv - Molecular Biology 2019Quote: ATM protein expression was assessed by SDS-PAGE and western blot using a rabbit anti-ATM antibody (1:1000; 2873S; Cell Signaling). Horseradish peroxidase-conjugated α-rabbit antibody (1:5000 ...
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bioRxiv - Cancer Biology 2019Quote: ... The resulting phosphorylated RB protein was detected by western blotting using anti-p-Rb (S807/S811) antibody (Cell Signaling Technology, 8516S).
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bioRxiv - Cell Biology 2021Quote: ... Protein accumulation rate upon proteasome inhibition was measured after 3-hour treatment with 10μM of MG-132 (Cell Signaling Technology #2194) in the RPMI medium.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to nitrocellulose using standard methods and membranes were blocked in 5% non-fat dry milk (Cell Signaling Technology) in TBS with 0.2% Tween-20 (Boston BioProducts) ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2021Quote: ... 100ug of protein was incubated with 6 uL of ubiquitin antibody and protease/phosphatase inhibitors (Cell Signaling Technologies, Danvers, MA, USA) overnight ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... For immunoprecipitation assays, cell lysates were incubated with Protein G Agarose (cat # 20398, Pierce) and anti-Flag antibody (cat # 2368, Cell Signaling) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... 10-15 μg of proteins were separated by SDS-PAGE and immunoblotted using the following antibodies: p-ERK1/2 (9101, Cell Signaling), ERK1/2 (4695 ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of relative densitometry was obtained by normalizing to the background and to loading control proteins (ACTB, from Cell Signaling Technology) using Image Lab Software (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... an amount of digested lysate equivalent to 10 mg of protein was subjected to ubiquitin remnant immunoprecipitation according to the manufacturer’s protocol (Cell Signaling Technologies). For phosphorylation analysis ...
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bioRxiv - Molecular Biology 2020Quote: Western blot was performed after chemoenzymatic labeling to detect O-GlcNAcylated proteins using α-streptavidin-HRP (Cell Signaling Technologies, Danvers, MA) (1:3000) ...
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bioRxiv - Plant Biology 2021Quote: ... and the interaction between coexpressed proteins was examined by immunoblot analyses using a mouse anti-Myc monoclonal antibody (1:10,000; Cell Signaling Technology), a rat anti-RFP monoclonal antibody (1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... Lysed samples were centrifuged and incubated for 3 h with Pierce Protein A/G Magnetic beads (88802) which were pre-incubated over night with V5 antibody (Cell signaling E9H80 ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with antibody that recognizes proteins IDH 1 (1:1000) and IDH 2 (1:1000) (Cell Signaling, MA) and α-Tubulin (Abcam ...
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bioRxiv - Plant Biology 2019Quote: ... SDS-PAGE gels and the interaction between co-expressed proteins determined by immunoblot analysis using a mouse anti-MYC monoclonal antibody (1:10,000; Cell Signaling Technology) and a mouse anti-GFP monoclonal antibody (1:5,000 ...