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Citations for Cell Signaling Technology :
501 - 550 of 768 citations for Protein Detection since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: Total protein was extracted from cells or tissues using cell lysis buffer (Cell Signaling, Cat No. 9803), containing 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies used were rabbit anti-phospho-S6 ribosomal protein (Ser235/236, 1:200; Cell Signaling 4858), mouse anti-NeuN (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Total protein lysates were analyzed by western blotting with the following antibodies (all from Cell Signaling Technology): phospho-ALK Y1604 (3341) ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with 1xPermbuffer III containing monoclonal antibodies against phosphorylated-S6-Ribosomal protein (D57.2.2E, Cell Signaling) or phosphorylated-mTOR (clone MRRBY ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates containing 400-500 μg protein were incubated with rabbit anti-human TREM2 antibody (Cell Signaling, 91068) / Dynabeads protein A (Thermo fisher ...
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bioRxiv - Genetics 2022Quote: ... Myc-tagged Rrp4 proteins were detected with mouse anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP ...
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bioRxiv - Genetics 2022Quote: ... and Myc-tagged Rrp4 proteins were detected with anti-Myc monoclonal antibody 9B11 (1:2000; Cell Signaling). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was isolated by immunoprecipitation using a mouse anti-His antibody (Cell Signaling, catalog #: 2366S) followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems ...
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bioRxiv - Molecular Biology 2022Quote: ... and associated proteins were harvested from top layer with RIPA buffer containing phosphatase inhibitor cocktail (Cell Signaling), and protease inhibitor cocktail (Sigma).
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bioRxiv - Cell Biology 2022Quote: ... 90 μg of protein was used for western blotting with cleaved caspase-3 (Cell Signaling Technologies, 9661), actin (Sigma ...
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bioRxiv - Immunology 2022Quote: Antibodies against the following proteins were used in Figure S1: Phospho-Rb (Ser807/811) (Cell Signaling, #9308), Cyclin B1 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit polyclonal phospho-S6 ribosomal protein (S240/244) (1/2000; Cell Signaling, Beverly, MA #5364, RRID:AB 10694233) was incubated 48 hours at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 3-day differentiated SKM cells using cell lysis buffer (9803S, Cell Signaling Technology), supplemented with a complete protease and phosphatase inhibitor cocktail (78442 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were prepared by mixing 10 to 20 μg of protein with Blue loading buffer (Cell Signaling) and 40 mM DTT (Cell Signaling) ...
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bioRxiv - Genetics 2023Quote: ... Immune complexes were precipitated using either protein A or G agarose beads (Cell Signaling Technology, Danvers, MA) for 4 h at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was pulled down overnight using Invitrogen Protein A Dynabeads with antibodies for H3 (Cell Signaling Technology), H3K27me3 (Abcam ...
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bioRxiv - Synthetic Biology 2024Quote: Surface-expressed proteins were assayed using Alexa Fluor 647 Anti-Myc tag antibody (Cell Signaling Technologies #2233S). T cell activation was assessed using Alexa Fluor 647 anti-CD25 (Biologend #302618) ...
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bioRxiv - Molecular Biology 2024Quote: Protein extract was isolated from cells using 1x Cell Signaling Lysis Buffer (Cell Signaling, Danvers, MA, USA), supplemented with 1x Halt™ Protease Inhibitor Cocktail EDTA-free (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... anti-Microtubule-associated protein 1 light chain B (anti-MAPLC3/LC3; for western blot: Cell Signaling Technology-CST ...
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bioRxiv - Cell Biology 2023Quote: ... the protein-DNA crosslinks in the immunoprecipitates were uncoupled using ChIP Elution Buffer (7009, Cell Signaling Technology). The DNA was subsequently purified using DNA purification columns ...
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bioRxiv - Physiology 2024Quote: ... Data were normalized for the expression of the housekeeping protein β-actin (cs4967, Cell Signaling, 1:5000).
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bioRxiv - Molecular Biology 2024Quote: 10μL of Protein G Dynabeads bound to 2.5μL of anti-HA monoclonal antibody (C29F4, Cell Signaling Technology) or 5μL of anti-ALG-1 polyclonal antibody (homemade ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and the total amount of each protein was detected by immunoblotting with antibodies against EphA2 (D4A2, Cell Signaling), EGFR (#4267 ...
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bioRxiv - Immunology 2019Quote: ... Equal amounts of protein were subjected to do immunoprecipitation using anti-DAP12 Ab (Cell Signaling Technology, Danvers, MA) and protein A/G plus agarose beads (Pierce Classic IP Kit ...
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bioRxiv - Synthetic Biology 2021Quote: Surface expressed proteins were assayed for using Alexa Fluor 647 Anti-Myc tag antibody (Cell Signaling Technologies #2233S). Jurkat activation was assessed using Alexa Fluor 647 anti-CD25 (Biologend #302618 ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred to nitrocellulose membranes and immunoblotted using mouse monoclonal Cas9 antibody (7A9-3A3, Cell Signaling Technology), HRP-conjugated rabbit monoclonal GAPDH antibody (D16H11 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitated proteins were resolved by acrylamide gel electrophoresis and immunoblotted with antibodies against either acetylated lysine (Cell Signaling) or malonylated lysine (Cell Signaling) ...
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bioRxiv - Cell Biology 2020Quote: Protein lysates were prepared using 1X RIPA analysis (10X RIPA buffer, #9806, Cell Signaling Technologies, Danvers, MA, USA) containing protease and phosphatase inhibitors (#5872S ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell lysates were immunoprecipitated using HIF-1α antibody and protein A-magnetic beads (Cell Signaling Technology, 73778) during overnight incubation at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the total protein was detected by using specific antibodies (GRK2: Santa Cruz sc-13143; GRK3: Cell signaling technology #80362 ...
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bioRxiv - Neuroscience 2020Quote: ... and a-Tubulin proteins were visualized by chemiluminescence after exposure to the anti-SMN antibody (Cell Signaling 2F1) following by an HRP linked secondary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were transferred to PVDF membrane and subjected to immunoblot analysis with anti-LGALS1 (#12936; Cell Signaling Technologies) and pan 14-3-3 loading control (#8312 ...
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bioRxiv - Genomics 2022Quote: ... USA and the mouse monoclonal antibody anti-Glial fibrillary acidic protein (GFAP) was purchased from Cell Signaling Technology, USA for immunohistochemistry (IHC) ...
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bioRxiv - Biochemistry 2022Quote: ... PP2Ac and Actin protein levels were detected by anti-PP2Ac antibody (Cell signaling Technology, Cat#: 2259, 1:1000) and anti-β-Actin antibody (Cell signaling Technology ...
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bioRxiv - Genomics 2019Quote: Protein lysates were obtained using ice-cold 1X RIPA buffer containing protease and phosphatase inhibitors (Cell Signaling 9806), and freshly supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Immunology 2020Quote: ... The DNA-protein complexes were immunoprecipitated using 5.0 μl per sample of STAT5 antibody (9363S, Cell Signaling Technology), and IgG (2729P ...
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bioRxiv - Microbiology 2020Quote: ... the proteins were probed overnight with rabbit anti-GFP antibody (1:2,000; clone D5.1, Cell Signaling Technology, USA) and revealed with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins separated by SDS-PAGE and analyzed by Western blotting using relevant antibodies (Cell Signaling: anti-SMAD1 #9743), anti-P-SMAD1/5 #9516 ...
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bioRxiv - Immunology 2019Quote: ... and eukaryotic translation initiation factor 4Ebinding protein 1 (4E-BP1) phosphorylated on Thr37/46 (236B4, Cell Signaling Technology). A viability dye was included in every panel (LIVE/DEAD Near-IR ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total protein kinase B antibody (AKT 1:1000, CST2920, CST4060, Cell Signaling Technology, Danvers, MA), anti-phospho and total IkB kinase antibody (p/total IkB 1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-phospho-s6 ribosomal protein (pS6 Ser240/244) (rabbit monoclonal, catalog #2215S, Cell Signaling Technology, MA, RRID: AB_331682). Secondary Alexa Fluor 594 (goat anti-rabbit ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were the following: anti-NOTCH1 targeting C terminus of the protein (Cell signaling, catalog no. 3608); anti-NOTCH2 targeting C terminus of the protein (Cell signaling ...
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bioRxiv - Microbiology 2020Quote: ... rabbit anti S6 ribosomal protein (5G10; 1:103 dilution in 5% BSA, Cell Signaling Technology, Leiden, The Netherlands). Secondary Abs. ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies against the following proteins were used: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (clone 14C10) (#2118, Cell Signaling, USA), E-cadherin (clone HECD-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA and proteins were eluted from beads by adding 150 μL of 1X elution buffer (#7009, Cell Signaling) and heating at 65°C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected using human monoclonal primary antibodies anti-cleaved caspase-3 (1:250, clone 5A1E, Cell Signaling) and anti-β-actin (1:1,000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples of equal protein concentration were prepared in reducing and denaturing conditions (Cat. No. 7722, Cell Signaling Technology).
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bioRxiv - Physiology 2022Quote: ... blocked in 5 % BSA TBST for 1 h and reprobed with S6 ribosomal protein antibody (#2317, Cell Signaling) using the same protocol as above ...