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Citations for Cell Signaling Technology :
201 - 250 of 261 citations for CD28 Human Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The sections designated to be incubated with the human AR antibody (#5153, 1:1000, Cell Signaling Technology) were boiled in 10 mM citrate buffer for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... were coated overnight at 4°C with a rabbit anti-human CD4 antibody (Cell Signaling Technology, 93518) at a concentration of 2 μg/ml in PBS ...
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bioRxiv - Immunology 2021Quote: ... 96-well plates were coated with human ACE2 recombinant protein (1μg/ml, mFc tag; #83986, Cell Signaling Technology), and then blocked using PBS containing 5% skim milk for 2 h at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 1mg nuclear lysate in 1.5mL RIP Buffer was incubated with 2.5µg rabbit anti-human RBBP7 antibody (Cell Signaling) or rabbit IgG isotype control antibody (I5006 ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated overnight at 4°C with a rabbit anti-human phospho-H2AX antibody (Cell Signaling, #9718) diluted (1:200 ...
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bioRxiv - Cancer Biology 2019Quote: ... and rabbit anti-SNAI2 (1:500 for human sections and 1:400 for mice sections, Cell Signaling: 9585) were added and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-human DLL4 (Cat# 2589) and rabbit anti-RhoA (Cat# 2117) were purchased from Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-β-catenin (1:500 for human sections and 1:150 for mice sections; Cell Signaling: 8480); rabbit anti-TWIST (1:500 ...
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bioRxiv - Immunology 2020Quote: ... the 96 well plate was coated with human ACE2 recombinant protein (1μg/ml, mFc tag; Cell Signaling Technology). The plate was blocked with PBS containing 5% skim milk for 2 h at RT ...
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bioRxiv - Immunology 2022Quote: ... Protein detection was carried out using anti-human LC3-I/II antibody (Cell Signaling Technology, Danvers, MA, #12741).
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bioRxiv - Immunology 2022Quote: ... Proteins were detected using human monoclonal primary antibodies anti-cleaved caspase-3 (1:250, clone 5A1E, Cell Signaling) and anti-β-actin (1:1,000 ...
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bioRxiv - Microbiology 2022Quote: The antibodies with the indicated dilutions were as follows: anti-human ACE2 (Cell Signaling Technology, #74512, 1:1000), anti-ACE2 (Affinity ...
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bioRxiv - Molecular Biology 2023Quote: ... and was directly used for quantitative PCR with SimpleChIP human CDKN1A promoter primers (Cell Signaling Technology, MA, US). ChIP-qPCR data were normalized to input DNA and presented as percentage of input ...
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bioRxiv - Cancer Biology 2023Quote: ... RRID: AB_2335990) and rabbit anti-human pSTAT3 clone D3A7 (Cell Signaling Technologies Cat# 9145, Lot# 43 RRID: AB_2491009) raised against a synthetic phosphopeptide corresponding to residues surrounding Tyrosine 705 of murine STAT3 ...
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bioRxiv - Bioengineering 2023Quote: A human non-immune single-chain (scFv) library14 was sorted first against anti-Myc antibody (Cell Signaling, 2276S) to select for full-length scFv antibodies ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant mouse Ifng and human IFNG proteins (with carrier) were purchased from Cell Signaling Technology (catalog #39127 and 8901). They were reconstituted with sterile water at a concentration of 0.1 mg/ml ...
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bioRxiv - Immunology 2020Quote: The following reagents were used to stain human cells: α-TCF-1 PE (clone C63D9) from Cell Signaling Technology; α-CCR5–BV650 (clone 3A9) ...
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bioRxiv - Cancer Biology 2022Quote: ... and antibodies used—human c-MYC (D84C12) #5605 and alpha tubulin (DM1A) Mouse mAb #3873 from Cell Signaling Technologies—were previously described by our group39 ...
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bioRxiv - Cancer Biology 2021Quote: ... The purified DNA (2µl per reaction) was quantified by real-time PCR using SimpleChIP human MITF (Cell Signaling Technology) or human c-MYC primers (forward ...
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bioRxiv - Immunology 2021Quote: Intracellular markers used were Rab5A (early endosomal marker; mouse anti-human Rab5A; 1:200; Cell Signaling Technology Cat. #46449), Rab7 (late endosomal marker ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was incubated with α-mouse ACE2 (cross-reactive to human ACE2) (1:1,000; #38241 Cell Signaling Technology), α-human TMPRSS2 (1:1,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were stained overnight with rabbit anti-human ABCB1 (E1Y7S, mAb #13978; Cell Signaling Technology; dilution 1:400). Sections were reviewed independently by two researchers blinded to clinical data ...
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bioRxiv - Cancer Biology 2023Quote: ... 17 human lung ADC samples were performed IHC staining with an anti-CD38 antibody (Cell Signaling Technology, Cat #51000).
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bioRxiv - Neuroscience 2021Quote: Soluble protein was collected from cell pellets and human brain tissue by resuspension in Cell Lysis Buffer (Cell Signaling Technologies) supplemented with 10µM PMSF on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... a rabbit mAb raised against synthetic peptide corresponding to residues surrounding Ser518 of human Merlin protein from Cell Signaling Technology Cat # 13281 and a mouse monoclonal antibody ...
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bioRxiv - Pathology 2023Quote: ... Human lung tissue sections were stained with anti-CD31 antibody (3528, Cell Signaling Technology, Danvers, MA, USA 1:200 dilution), anti-ACKR1 antibody (NB100-2421 ...
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bioRxiv - Developmental Biology 2024Quote: ... which consists of Basal Human TS Cell Medium supplemented with 100 ng/mL of neuregulin 1 (NRG1, 5218SC, Cell Signaling), 7.5 μM A83-01 ...
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bioRxiv - Genetics 2022Quote: ... human anticentromere (1:100; Antibodies Inc., cat# 15-234) and mouse monoclonal anti-H3K9me2/3 (1:100, Cell Signaling, cat# 5327) primary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: Human tissue samples and cell preparations were harvested in Complete RIPA Buffer - 1× radio immunoprecipitation buffer (10× RIPA; 9806, Cell Signaling) and one tablet each of PhoStop and Complete Mini (04906845001 and 04693124001 ...
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bioRxiv - Cell Biology 2021Quote: Human paraffin embedded pancreas biopsies were sectioned to 3 μm and immunostained with antibodies against MAFB (Cell Signaling Technologies, cat#41019), ARX (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2022Quote: ... De-identified FFPE tumor specimens were cut into 5 μm sections and IHC performed as described above to stain for human EZH2 (5246, Cell Signaling) and NKp46 (AF1850 ...
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bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
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bioRxiv - Bioengineering 2019Quote: ... cardiac fibroblasts were pre-treated in plastic tissue-culture flasks with 10 nM human TGF-β1 (Cell Signaling Technology, cat# 8915LC) for 48h ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360 ...
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bioRxiv - Cancer Biology 2020Quote: ... Human-specific EpCam-647 (clone VU1D9, #5447) and rabbit anti-human γH2AX antibodies (Phospho-Histone H2A.X Ser139; clone 20E3, #9718) from Cell Signaling Technologies, and EpCam-APC antibody (clone EBA-1 ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...
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bioRxiv - Microbiology 2022Quote: ... we stimulated NFκB-dependent gene expression by treating cells with purified human TNF (100 ng per ml; Cell Signaling Technology, Inc.) in 1X Opti-MEM reduced serum media (Gibco Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with rabbit-anti-human ABCB1 (E1Y7B; mAb #13342; Cell signaling Technology; dilution 1:1000) and mouse-anti-human β-actin (sc-47778 ...
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Cancer Biology 2020Quote: ... Anti-Phospho-p44/42 MAPK (ERK1/2—Thr202/Tyr204, CAT# 9101) and anti-MCL-1 (D35A5 rabbit anti-human primary, CAT# 5453) antibodies were purchased from Cell Signaling Technology. Anti-ELP4 (rabbit anti-human primary ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Genomics 2020Quote: To assess editing efficiency,1 × 106 cells were taken from culture five days post electroporation and stained with the following primary anti-human antibodies: Cbl-b (Clone D3C12, Cell Signaling Technologies) followed by AlexaFluor 647 F(ab’)2 goat anti rabbit IgG (H+L ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies have been used on human PDAC tissue of figure 1b or mouse PDAC tissue from figure 2b: pan-Keratin (#4279, Cell Signaling Technology), p-ERK (#9101 ...
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bioRxiv - Pathology 2024Quote: ... The membranes were blocked with 5% non-fat milk in Tris-buffered saline with 0.1% Tween 20 (TBST) for 1 hour at room temperature and then incubated overnight at 4°C with antibodies recognizing human TIM4 (Cell signaling technology, #75484T ...
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bioRxiv - Cancer Biology 2019Quote: ... The immunohistochemical staining was also conducted by incubating of indicated sections with distinct primary antibodies against human Nrf1 (from Cell Signaling Technology, Inc.), β-catenin and Cyclin D1(both obtained from Epitomics ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Bioengineering 2023Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...