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1 - 50 of 261 citations for CD28 Human Cynomolgus HEK293 Fc since 2019

• The CD28-transmembrane domain mediates chimeric antigen receptor heterodimerization with CD28

  Yannick D. Muller, et al., bioRxiv - Bioengineering 2020

  Quote: ... rabbit anti-human CD28 (clone D2Z4E, Cell Signaling) followed by anti-rabbit IgG Pierce™ protein A/G magnetic beads (Thermo Fisher ...
• Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairment

  Kenji Ichikawa, Kazuhisa Ichikawa, bioRxiv - Molecular Biology 2022

  Quote: ... CD28 (Cell Signaling, 38774), ICOS (Cell Signaling ...
• The CD28-transmembrane domain mediates chimeric antigen receptor heterodimerization with CD28

  Yannick D. Muller, et al., bioRxiv - Bioengineering 2020

  Quote: ... rabbit anti-CD28 (clone D2Z4E, Cell Signaling), HRP-conjugated anti-mouse IgG (Cell Signaling ...
• Phosphatidylserine binding regulates TIM-3 effects on T cell receptor signaling

  Courtney M. Smith, et al., bioRxiv - Biochemistry 2021

  Quote: ... anti-CD28 pY191 (Cell Signaling Technology, catalog #16399), anti-AKT pT308 (Cell Signaling Technology cat# 2965) ...
• Retrograde And Anterograde Transport Of LAT-Vesicles During The Immunological Synapse Formation: Defining The Finely-Tuned Mechanism

  Juan José Saez, et al., bioRxiv - Cell Biology 2020

  Quote: ... rabbit anti-CD28 (1:1000, 38774, Cell Signaling), rabbit anti-GMAP210 (Gift from Michel Bornens) ...
• BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ

  Jaehyeon Jeong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HEK293 lysates were collected using RIPA buffer (Cell Signaling Technology), and the protein concentrations of the lysates were quantified through Bradford assays (Bio-Rad ...
• ERK2-topoisomerase II regulatory axis is important for gene activation in immediate early genes

  Heeyoun Bunch, et al., bioRxiv - Molecular Biology 2023

  Quote: ... HEK293 cell extracts were prepared using a RIPA buffer (Cell Signaling Technology) in the presence of freshly added protease inhibitors [1 mM benzamidine ...
• The Sequence Basis for Selectivity of Ephrin-B2 Ligand for Eph Receptors and Pathogenic Henipavirus G Glycoproteins

  Krishna K. Narayanan, et al., bioRxiv - Microbiology 2023

  Quote: ... A competitor solution was prepared by mixing soluble human IgG1-Fc fused Eph receptor/s (150 nM sEphB2-Fc or 1 μM sEphB3-Fc plus 1μM sEphB4-Fc) and anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
• Hypoxia is a dominant remodeler of the CD8⁺ T cell surface proteome relative to activation and regulatory T cell-mediated suppression

  James R. Byrnes, et al., bioRxiv - Immunology 2021

  Quote: ... CD8+ and CD4+ T effectors were stimulated by addition to tissue culture flasks coated with anti-CD3 (produced in-house, clone OKT3) and anti-CD28 ([D2Z4E], Cell Signaling) antibodies or with anti-CD3/anti-CD28 Dynabeads (Thermo ...
• LPA/PKD-1 signaling promotes development of arteriolar niche that supports self-renewal of breast cancer stem cells and stemness

  Yinan Jiang, et al., bioRxiv - Cancer Biology 2020

  Quote: ... mouse anti-human/rat CD44 (5640S) and rabbit anti-human/mouse ALDH1A1 (12035S) (Cell Signaling Technology), rabbit anti-human/mouse CD36 (ab133625 ...
• Loss of TIM4-Dependent Efferocytosis in Kupffer Cells Promotes Liver Fibrosis in Nonalcoholic Steatohepatitis

  Hongxue Shi, et al., bioRxiv - Pathology 2024

  Quote: ... human liver sections were incubated at 4 °C overnight with anti-human TIM4 (Cell signaling technology, #75484T ...
• Multi-organ signaling mobilizes tumor-associated erythroid cells expressing immune checkpoint molecules

  Yasuyo Sano, et al., bioRxiv - Cancer Biology 2020

  Quote: ... human PDL1 (E1L3N; Cell Signaling Technology), and EF5 (ELK3-51 ...
• A mosaic renal myeloid subtype with T-cell inhibitory and protumoral features is linked to immune escape and survival in clear cell renal cell cancer

  Dorothee Brech, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit-anti-human FOXO1 (Cell Signaling) and mouse-anti-human CD3 (UCHT1 ...
• Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins

  Takeshi Uemura, et al., bioRxiv - Neuroscience 2020

  Quote: ... or human IGF-I (Cell Signaling) was applied to the cultured CGCs at DIV2 ...
• Antibody-induced glomerulonephritis is amplified by RTEC-intrinsic IL-17 signaling and restrained by IL-17-mediated induction of the endoribonuclease Regnase-1 (Zc3h12a)

  De-Dong Li, et al., bioRxiv - Immunology 2021

  Quote: ... human anti-Iκbζ (9244, Cell signaling), human/mouse anti-YY1 (sc-7341 ...
• Autophagy mediates cancer cell resistance to doxorubicin induced by the Programmed Death 1/Programmed Death Ligand 1 immune checkpoint axis

  Lori Minassian, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 30 minutes prior to addition of rPD-1-Fc (dosage according to Cell Signaling Technologies (CST) protocol ...
• pHLARE: A Genetically Encoded Ratiometric Lysosome pH Biosensor

  Bradley A. Webb, et al., bioRxiv - Cell Biology 2020

  Quote: ... and human LAMP1 (Cell Signaling Technologies; 9091) diluted in blocking buffer ...
• ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

  Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

  Quote: ... anti-mouse/human STING (Cell Signaling #13647), anti-human phospho-IRF3 (Abcam ab196035) ...
• ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

  Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human phospho-STING (Cell Signaling #19781), anti-mouse phospho-STING (Cell Signaling #72971) ...
• ABCC1/MRP1 exports cGAMP and modulates cGAS-dependent immunity

  Joanna H. Maltbaek, et al., bioRxiv - Immunology 2021

  Quote: ... anti-mouse/human Actin (Cell Signaling #3700), anti-human phospho-STING (Cell Signaling #19781) ...
• S1P-S1PR1 activity controls VEGF-A signaling during lymphatic vessel development

  AM Golding-Ochsenbein, et al., bioRxiv - Developmental Biology 2019

  Quote: ... rabbit anti-human Rab5 (Cell Signaling, #3547P). After an intense wash step in 0.1% Triton-X PBS ...
• Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of Post-Transcriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis

  Katherine E. Uyhazi, et al., bioRxiv - Developmental Biology 2019

  Quote: ... CDK4 monoclonal mouse anti-human (Cell Signaling), Cyclin D1 monoclonal mouse anti-human (Cell Signaling) ...
• Nuclear soluble cGAS senses DNA virus infection

  Yakun Wu, et al., bioRxiv - Immunology 2021

  Quote: ... anti-human cGAS (Cell Signaling Technology, #79978), anti-mouse cGAS (Cell Signaling Technology ...
• APP binds to the EGFR ligands HB-EGF and EGF, acting synergistically with EGF to promote ERK signaling and neuritogenesis

  Joana F. da Rocha, et al., bioRxiv - Neuroscience 2020

  Quote: ... Human recombinant EGF was from Cell Signaling and was used at a 100 ng/mL working concentration (WC) ...
• Splice switching oligonucleotide mediated gene knockdown in B cells and plasma cells

  Anne Marchalot, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit anti-human RelA antibody (Cell Signaling) or mouse anti-beta-actin antibody (Sigma) ...
• Bone morphogenetic protein 4 reduces global H3K4me3 to inhibit proliferation and promote differentiation of human neural stem cells

  Sonali Nayak, et al., bioRxiv - Neuroscience 2020

  Quote: ... Human OCT4 (Cell Signaling Technology, Cat#4641) and CCND1 (Cell Signaling Technology ...
• Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

  Thomas Chapman, et al., bioRxiv - Immunology 2019

  Quote: ... rabbit anti-human p38 #9212 (Cell Signaling), rabbit anti-human LAMTOR1 #8975 D11H6 (Cell Signaling) ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human pTBK1 (#5483, Cell Signaling), rabbit-anti human STING (#13647S ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human pSTING (#19781 Cell Signaling), rabbit-anti human pTBK1 (#5483 ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human TBK1 (#3504, Cell Signaling) or rabbit-anti human TREX1 (#185228 ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human STING (#13647S, Cell Signaling), rabbit-anti human TBK1 (#3504 ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human pIRF3 (#29047, Cell Signaling), rabbit-anti human pSTING (#19781 Cell Signaling) ...
• A base-line cellular antiviral state is maintained by cGAS and its most frequent naturally occurring variant rs610913

  Julia Kazmierski, et al., bioRxiv - Immunology 2021

  Quote: ... rabbit-anti human IRF3 (#4302, Cell Signaling), rabbit-anti human pIRF3 (#29047 ...
• Mechano-signaling of prostate tumor initiating cells facilitates their tropism to stiff metastatic niche

  Lanpeng Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-human phosphorylated Paxillin (Cell Signaling Technology) and anti-humanKi67 (Abcam) ...
• The V-ATPase-ATG16L1 axis recruits LRRK2 to facilitate lysosomal stress responses

  Tomoya Eguchi, et al., bioRxiv - Molecular Biology 2023

  Quote: ... anti-human LAMP1 [D2D11] (Cell Signaling Technology), anti-mouse LAMP1 [1D4B] (Bio-Rad) ...
• Efficient enzyme-free isolation of brain-derived extracellular vesicles

  A. Matamoros-Angles, et al., bioRxiv - Neuroscience 2024

  Quote: ... CD81 human-specific (D3N2D) (#56039S, Cell Signaling) and mouse-specific (D502Q ...
• p38α-MAPK-deficient myeloid cells ameliorate symptoms and pathology of APP-transgenic AD mice

  Qinghua Luo, et al., bioRxiv - Neuroscience 2021

  Quote: ... Human Aβ was stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and microglia labeled with rabbit anti-Iba-1 antibody (Wako Chemicals ...
• A rectal cancer model establishes a platform to study individual responses to chemoradiation

  Karuna Ganesh, et al., bioRxiv - Cancer Biology 2019

  Quote: ... rabbit anti-human EPCAM (Cell Signaling, cat. #2626), rabbit anti-collagen IV (Serotec ...
• Chlorotoxin Redirects Chimeric Antigen Receptor T Cells for Specific and Effective Targeting of Glioblastoma

  Dongrui Wang, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit-anti-human-PDL1 (Cell Signaling Technology, E1L3N). The slides for IHC assays were scanned via a NanoZoomer 2.0-HT Digital slide scanner (Hamamatsu ...
• Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of Post-Transcriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis

  Katherine E. Uyhazi, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Cyclin D1 monoclonal mouse anti-human (Cell Signaling), Sox2 goat anti-mouse (Santa Cruz) ...
• Pumilio Proteins Exert Distinct Biological Functions and Multiple Modes of Post-Transcriptional Regulation in Embryonic Stem Cell Pluripotency and Early Embryogenesis

  Katherine E. Uyhazi, et al., bioRxiv - Developmental Biology 2019

  Quote: ... Cyclin A polyclonal mouse anti-human (Cell Signaling), CDK4 monoclonal mouse anti-human (Cell Signaling) ...
• Developing Small Molecules that Inhibit K-Ras/GTP Binding Based on New Affinity Measurements

  Luca Carta, et al., bioRxiv - Cancer Biology 2020

  Quote: ... mouse mAb anti-human RAS (Cell Signaling Technology). Compounds SHY-855 and SHY-867 were synthesized at Enamine LLC (Kiev ...
• Hydroxynonenal causes Langerhans cell degeneration in the pancreas of Japanese macaque monkeys

  Piyakarn Boontem, Tetsumori Yamashima, bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... rabbit anti-human cathepsin B (Cell Signaling, USA), and mouse monoclonal anti-Lamp2 (Abcam ...
• Low Level Whole-Brain Radiation Enhances Theranostic Potential Of Single Domain Antibody Fragments For HER2-Positive Brain Metastases

  Daniele Procissi, et al., bioRxiv - Cancer Biology 2022

  Quote: ... rabbit anti-human GAPDH (Cell Signaling Technologies, MA), and secondary goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP ...
• Liver Endothelium Microenvironment Promotes HER3-mediated Cell Growth in Pancreatic ductal adenocarcinoma

  Wei Zhang, et al., bioRxiv - Cancer Biology 2022

  Quote: ... Ki67 (human) (1:200, Cell Signaling Technology, 9449T). Isotype-matched immunoglobulin was used as a control ...
• Human milk extracellular vesicles target nodes in interconnected signalling pathways that enhance oral epithelial barrier function and dampen immune responses

  Marijke I. Zonneveld, et al., bioRxiv - Immunology 2020

  Quote: ... rabbit anti-human TLR3 (D10F10, Cell Signaling TECHNOLOGY, dilution 1.1000 ...
• Single cell spatial analysis identifies regulators of brain tumor initiating cells

  Reza Mirzaei, et al., bioRxiv - Cancer Biology 2022

  Quote: ... human Ki67 (1:400; Cell Signaling Technology, 9449), mouse Ki67 (1:500 ...
• Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

  Thomas Chapman, et al., bioRxiv - Immunology 2019

  Quote: ... rabbit anti-human TBK1 #3504 D1B4 (Cell Signaling), rabbit anti-human RIPK2 #4142 D10B11 (Cell Signaling) ...
• Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

  Thomas Chapman, et al., bioRxiv - Immunology 2019

  Quote: ... rabbit anti-human RIPK2 #4142 D10B11 (Cell Signaling), rabbit anti-human p38 #9212 (Cell Signaling) ...
• Ataxin-3 links NOD2 and TLR2 mediated innate immune sensing and metabolism in myeloid cells

  Thomas Chapman, et al., bioRxiv - Immunology 2019

  Quote: ... rabbit anti-human LAMTOR1 #8975 D11H6 (Cell Signaling), rabbit anti-human PLD3 HPA012800 (Sigma) ...