Dl Serine 1 13C 99% citations for Cell Signaling Technology

Nutrient deprivation differentially affects gene expression, immunity, and pathogen susceptibility across symbiotic states in a model cnidarian

Maria Valadez-Ingersoll, et al., bioRxiv - Genomics 2023

Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...

polo affects cell fate upon ionizing radiation in Drosophila hematopoietic progenitors by negatively regulating lok

Tram Thi Ngoc Nguyen, et al., bioRxiv - Developmental Biology 2023

Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...

Transcriptional suppression of sphingolipid catabolism controls pathogen resistance in C. elegans

Mohamad A. Nasrallah, et al., bioRxiv - Immunology 2023

Quote: ... blocked with 5% fat-free milk in TBST and probed with a 1:1000 dilution of an antibody that recognizes the doubly-phosphorylated TGY motif of PMK-1 (Cell Signaling Technology) or a monoclonal anti-tubulin antibody (Sigma-Aldrich ...

Systemic Lupus Erythematosus Serum Stimulation of Human Intestinal Organoids Induces Changes in Goblet Cell Differentiation and Mitochondrial Fitness

Inga Viktoria Hensel, et al., bioRxiv - Cell Biology 2023

Quote: ... The membrane was blocked with 5% milk in TBST for 1 h at room temperature and then probed overnight at 4°C with pStat1 (1:1000; Cell Signaling #9167S), β-actin (1:500 ...

Radiation therapy promotes unsaturated fatty acids to maintain survival of glioblastoma

Mara De Martino, et al., bioRxiv - Cell Biology 2022

Quote: ... m-h-anti-CHOP 1:1000, m-h-anti-fatty acid synthase 1:100, cat#3180T; m-h-anti-vinculin 1:1000, cat#13901S; Cell Signaling Technology) at 4°C overnight ...

A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments

Christopher Chidley, et al., bioRxiv - Cancer Biology 2023

Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...

H3.1^Cys96 oxidation by mitochondrial ROS promotes chromatin remodeling, breast cancer progression to metastasis and multi-drug resistance

Flavio R. Palma, et al., bioRxiv - Cancer Biology 2022

Quote: ... Membranes were incubated with appropriate primary antibodies (phospho-ATM, 1:1000, Cell Signaling Technology, #13050; E-Cadherin, 1:1000, Cell Signaling Technology, #3195 ...

Regulation of Store-Operated Ca²⁺ Entry by IP₃ Receptors Independent of Their Ability to Release Ca²⁺

Pragnya Chakraborty, et al., bioRxiv - Cell Biology 2023

Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...

SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure

Luming Wan, et al., bioRxiv - Cell Biology 2023

Quote: ... anti-phospho-IRF3 (4947S, 1:1,000 dilution) and anti-phospho-TBK1 (5483T, 1:2,000 dilution) were purchased from Cell Signaling Technology (Massachusetts, USA). Anti-phospho-p65 (A19653 ...

Liraglutide protects against diastolic dysfunction and improves ventricular protein translation

Cody Rutledge, et al., bioRxiv - Physiology 2023

Quote: ... for 1 hour and then incubated overnight at 4°C with primary antibodies p-S6 (Ser240/244, 1:1000, Cell Signaling, #2215), S6 (1:1000 ...

Quantitative flow cytometric selection of tau conformational nanobodies specific for pathological aggregates

Jennifer M. Zupancic, et al., bioRxiv - Bioengineering 2023

Quote: ... Yeast cells were combined with QD-fibril complexes in a total volume of 200 μL with 1% milk and 1:1000 mouse anti-Myc antibody (Cell Signaling, 2276S) and allowed to incubate with end-over-end mixing at room temperature for approximately 3 h ...

Astrocytic Foxo1 regulates hippocampal spinogenesis and synaptic plasticity to enhance fear memory

João Filipe Viana, et al., bioRxiv - Neuroscience 2023

Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...

SPLIT HAND/FOOT VARIANTS FAIL TO RESCUE PRDM1A MUTANT CRANIOFACIAL DEFECTS

Brittany T. Truong, et al., bioRxiv - Developmental Biology 2023

Quote: ... and incubated overnight at 4°C with rotation in the respective antibody (IgG [1:100; Jackson ImmunoResearch, 111-005-003, RRID: AB_2337913], H3K27ac [1:66; Cell Signaling Technology, 4353S ...

Injury induced renal fibrosis promotes cystogenesis and cyst growth in adult mice with autosomal dominant polycystic kidney disease

Ming Wu, et al., bioRxiv - Pathology 2023

Quote: ... 0.01%Tween 20) for 1 hour at room temperature and was followed by incubation with anti-EZH2 (1:1000, 5246S, Cell Signaling Technology) antibody ...

A TrkB and TrkC partial agonist restores deficits in synaptic function and promotes activity-dependent synaptic and microglial transcriptomic changes in a late-stage Alzheimer’s mouse model

Amira Latif-Hernandez, et al., bioRxiv - Neuroscience 2023

Quote: ... rabbit polyclonal CaMKII (1:2000, 3362), rabbit monoclonal anti-phopho-GluA1Ser831 (1:2000, 75574) and rabbit monoclonal GluA1 (1:2000, 13185) purchased from Cell Signaling Technology, Inc. ...

Inhibiting endothelial cell Mst1 attenuates acute lung injury in mice

Zhi-Fu Guo, et al., bioRxiv - Physiology 2023

Quote: ... 1:1000 dilution), antianti-p-MOB1 (#8699, 1:1000 dilution), and anti-MOB1 (#13730, 1:1000 dilution) antibodies were purchased from Cell Signaling Technology. Anti-phospho-Mst1 (AP0906 ...

Synaptic Activity Causes Minute-scale Changes in BAF Complex Composition and Function

S. Gourisankar, et al., bioRxiv - Molecular Biology 2023

Quote: ... then stained with 1:2000 chicken anti-GFP (Aves #GFP-1020) for visualization of dendrites and 1:3200 rabbit anti-cFos (Cell Signaling 9FG) to validate stimulation ...

The renal capsule, a vibrant and adaptive cell environment of the kidney in homeostasis and aging

Ben Korin, et al., bioRxiv - Immunology 2023

Quote: ... The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100, 78060S, Cell Signaling Technology) in Ce3D™ Antibody Diluent Buffer (48 hr ...

SAP30BP interacts with RBM17/SPF45 to promote splicing in a subset of human short introns

Kazuhiro Fukumura, et al., bioRxiv - Molecular Biology 2023

Quote: ... anti-SF3B1 (1:500 dilution; D221-3, MBL Life Science) and anti-phosph-SF3B1(Thr313) (1:500 dilution; D8D8V, Cell Signaling Technology). After washing three times with PBS ...

GCN5L1-mediated acetylation prevents Rictor degradation in cardiac cells after hypoxic stress

Paramesha Bugga, et al., bioRxiv - Cell Biology 2023

Quote: ... Cat. 97166 [D4C6R], mouse mAb, 1:1000; Akt, Cell Signaling, Cat. 4685 [11E7], rabbit mAb, 1:1000; p-Akt [Ser473], Cell Signaling, Cat ...

Leveraging the p53 signaling pathway as a radio-sensitization strategy in endometrial cancer

Roberto Vargas, et al., bioRxiv - Cancer Biology 2023

Quote: ... 1:3000), anti-p21 Waf1/Cip1 (clone 12D1, #2947, 1:3000), anti-Vinculin (clone E1E9V, #13901, 1:5000) were from Cell Signaling Technology. Goat anti-rabbit antibody linked to horseradish peroxidase (HRP ...

C-type natriuretic peptide improves maternally aged oocytes quality by inhibiting excessive PINK1/Parkin-mediated mitophagy

Hui Zhang, et al., bioRxiv - Developmental Biology 2023

Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174; rabbit anti-p62 antibody, 1:1000, Cell Signaling, Cat#23214 ...

T cell responsiveness to IL-10 defines the immunomodulatory effect of costimulation blockade via anti-CD154 and impacts transplant survival

Marcos Iglesias, et al., bioRxiv - Immunology 2024

Quote: ... Protein expression levels were detected with the following antibodies: Jak-1 (6G4) and phospho-Jak-1 (Tyr1034/1035) (from Cell Signaling Technology), IL-10Rα (A3 ...

Unravelling biological processess and EGFR pathway regulation by the receptor-like protein tyrosine phosphatase PTPRH in non-small cell lung cancer

Mylena M. O. Ortiz, et al., bioRxiv - Cancer Biology 2024

Quote: ... The coverslips were then incubated with primary antibodies against BioID2 (1:5000, BioFront #BID2-CP-100) or HA tag (1:800, Cell Signaling #3724) diluted in 1% BSA/ PBS-Tween 0.1% for one hour in a humidified chamber at RT ...

Inhibition of Inflammatory Osteoclasts Enhances CGRP⁺TrkA⁺ Signaling and Accelerates Callus Remodeling in Osteoporotic Fractures

Yuexia Shu, et al., bioRxiv - Pathology 2024

Quote: ... the cells are stained with specific fluorescent dyes using antibodies targeting Cx3cr1 (1:100, NBP2-68616, Novus) and Ly6C (1:100, 88920, Cell Signaling Technology). Once stained ...

Engineered 3D Hydrogel Matrices to Modulate Trophoblast Stem Cell-Derived Placental Organoid Phenotype

Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...

LEDGF interacts with the NID of MeCP2 and modulates MeCP2 condensates

Saskia Lesire, et al., bioRxiv - Molecular Biology 2024

Quote: ... Subsequently membranes were incubated with primary antibodies (1:500 rabbit anti-LEDGF-PWWP [Abcam; ab177159], 1:1000 rabbit anti-MeCP2 [Cell Signaling Technology; 3456S] ...

A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants

Aashima Khosla, et al., bioRxiv - Plant Biology 2020

Quote: ... and horse anti-mouse (1:5000, Cell Signaling, #7076). Blots were developed using an Azure Radiance Plus chemiluminescent substrate (#AC2102).

SARS-CoV-2 infection induces a pro-inflammatory cytokine response through cGAS-STING and NF-κB

Christopher J. Neufeldt, et al., bioRxiv - Microbiology 2020

Quote: ... Rabbit anti-IkB (Cell Signaling: 9242s, WB - 1:1000); Sheep and-TGN46 (Biorad:AHP500G ...

Nucleus translocation of tRNA synthetase mediates late integrated stress response