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Citations for Cell Signaling Technology :
4801 - 4850 of 10000+ citations for Dl Serine 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... and stained with 1:50 RBD-sfGFP expression medium (prepared as previously described and 1:250 anti-myc Alexa 647 (clone 9B11, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Bioengineering 2020Quote: ... and blocked with 1% BSA for 1 hour prior to incubation with primary antibodies raised against OCT4 (Cell Signaling, Cat. # 28901:800), NANOG (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with APC-conjugated CD45 (1:25 dilution; Miltenyi) and Alexa Fluor 488-conjugated pan-keratin (1:25 dilution, Cell Signaling Technology) antibodies for 10 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... 1:1,000, 9251), anti-c-jun (60A8, 1:1,000, 9165), anti-phospho-c-jun (S63, 1:1,000, 9261) (Cell Signaling, Beverly, MA, USA); anti-BECN1/Beclin-1 (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... from DSHB; anti-FoxO3 (1/1000; cat. no. 2497) and anti-phospho-Hsp27(Ser82) (1/1000; cat. no.2406) from Cell Signaling Technology; anti-HA (1/1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to western blot analysis using anti-Prdx5 (1:500, Ab Frontier) and anti-hnRNPK (1:500, Cell Signaling Technology) antibodies.
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bioRxiv - Cancer Biology 2022Quote: The following antibodies were used for immunoblotting (IB) at 1:1,000 unless otherwise stated: Anti-AXL (8661S, Cell Signaling, IHC 1:500), anti-phospho AXL y702 (5724 ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with 1:5 home-made anti-p53 (overnight at +4°C) and 1:100,000 anti-Lamin B1 (1 hr at room temperature) (Cell Signaling Technology, #12586). As secondary antibodies ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.1% Tween-20 in PBS) for at least 1 h and then incubated in 1:100 anti-pSmad1/5/9 (Cell Signaling, #13820) in FBS-PBST at 4°C overnight ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated for 1 hour at 25°C with a solution containing 1/400 goat α-rabbit-Alexa-488 (Cell Signaling 4412S) and 1 μM Hoechst 34580 to detect nuclear material (H21486 Molecular Probes. ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were further incubated with α-Syn rabbit polyclonal antibody 1:500 (US biological) followed by goat anti-rabbit secondary at 1:1000 (Cell signaling) after which treated with TMB substrate ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Cancer Biology 2020Quote: ... DyLight 800 conjugate (1:15000, #5257) and anti-rabbit IgG(H+L) DyLight 680 conjugate (1:15000, #5366) from Cell Signaling Technologies. The images were captured using Odyssey CLx Imager (LICOR Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... 5% BSA (AmericanBio) in TBST (TBS with Tween 20) for 1 h at RT and incubated with AKT (1:1000, rabbit, Cell Signaling 9272), pAKT (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... Cat no.: 4370; Dilution 1:1000) and total p44/42 MAPK (Cat no.: 4695; Dilution 1:1000) were obtained from Cell Signaling Technology. Horseradish peroxidase (HRP)-conjugated anti-rabbit (Cat no. ...
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bioRxiv - Physiology 2021Quote: ... A mouse primary anti-CaSR monoclonal antibody (1:2000, Gentex, Zeeland, MI) or a mouse primary anti-Myc (9B11) monoclonal antibody (1:1000, Cell Signaling Technology) was applied followed by a secondary horseradish peroxidase-coupled secondary antibody (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... rabbit anti-Stat3K685ac (2523S, 1:2000 dilution), and anti-rabbit IgG HRP conjugate (7074P2, 1:10000 dilution) were from Cell Signaling Technology. Anti-tubulin hFAB Rhodamine (12004166 ...
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bioRxiv - Developmental Biology 2021Quote: ... and Samples were washed extensively in PBS-Tx (6 × 1 hour) and incubated with anti-rabbit-HRP (Cell Signaling #7074, 1:1000) diluted in 1% BSA/PBS-Tx ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.5% Tween-20) and incubated 1 hour at room temperature with 1/2500 of primary antibody anti-pERK (Cat.#4377; Cell Signaling Technology) diluted saline buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 1 h incubation with the HRP-conjugated secondary antibody (Cell Signaling 7076S, 1:10,000 in 2.5% milk in TBS-T). After three washes in TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked with 1% BSA plus 10% goat serum for 1 hr and stained with the anti-phospho-paxillin (Tyr118) antibody (Cell Signaling Technology) at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Systems Biology 2022Quote: ... Membranes were probed with p-E2F1 (1:1,000 dilution; cat. no. MABE1782; MilliporeSigma) or E2F1 (1:1,000 dilution; cat. no. 3742S; Cell Signaling Technology, Beverly, MA) and ROCK2 (1:1,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... gels were transferred to Nitrocelllose membranes and probed with anti-CFTR (ab596 from Cystic Fibrosis Foundation, 1:1750) or anti-Nav 1.5 (D9J7S, Cell Signaling, 1:5000) overnight in 5% milk in TBST ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h at room temperature and blotted with an antibody against phosphorylated upstream frameshift protein 1 (phospho-UPF1) (pUPF1; 1:1000, Cell Signaling Technologies) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... p-S6S235/236 (#2211; 1:500) and p-4EBP1T37/46 (#2855; 1:1000) antibodies were obtained from Cell Signaling (Danvers, MA, USA). Cre antibody (NB100-56135 ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:3000), anti-p21 Waf1/Cip1 (clone 12D1, #2947, 1:3000), anti-Vinculin (clone E1E9V, #13901, 1:5000) were from Cell Signaling Technology. Goat anti-rabbit antibody linked to horseradish peroxidase (HRP ...
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bioRxiv - Systems Biology 2023Quote: ... Anti-caspase-8 antibody (4790, dilution 1:1000) and anti-cleaved caspase-8 antibody (8592, dilution 1:500) were purchased from Cell Signaling Technology. Anti-p-RIP3 antibody (ab222320 ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... The sections were incubated with primary antibody (pS6 Cell Signaling 2215, 1:1000; GFAP Cell Signaling #3670, 1:500; NeuN Cell Signaling D4G40 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed in TTBS/1% skim milk, incubated with secondary antibodies (1:5000 anti-mouse IgG, HRP-linked antibody (Cell Signaling #7076S)) ...
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bioRxiv - Systems Biology 2023Quote: ... rinsed in wash buffer and incubated for one hour in the primary antibodies (PAK4 Santa Cruz Biotechnology sc-390507 1:75 primary dilution, TGFBI Abcam ab170874 1:300 primary dilution, DDR1 Cell Signaling #5583 primary dilution 1:2000 ...