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Citations for Cell Signaling Technology :
351 - 400 of 790 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Mouse BM-APCs were lysed in 1X Cell Lysis buffer (Cell signaling technologies, Catalog #9803) or EDTA free lysis buffer (50 ...
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bioRxiv - Immunology 2020Quote: ... BM-APCs were lysed using Cell Lysis Buffer (Cell Signaling Technologies, Danvers, MA, Catalog # 9803S) supplemented with PMSF (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The bacteria attached to the HeLa cells were lysed using a lysis buffer (Cell Signaling), followed by concentration using an Amicon Ultra-0.5 centrifugal filter apparatus (Merck KgaA) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibody and 10X CST Cell Lysis Buffer (Cell Signaling Technology, to 1X final concentration) were added to each IP sample ...
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bioRxiv - Biochemistry 2021Quote: Cell lysate was diluted to 1mg/mL in 1x cell lysis buffer (Cell Signaling Technologies), supplemented with 0.5% SDS ...
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bioRxiv - Biochemistry 2021Quote: ... HRP-linked antibody (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #7074) was incubated at 4 °C for 1h ...
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bioRxiv - Cell Biology 2021Quote: ... Cultured cells were lysed in a RIPA buffer (Cat. 9806s, Cell Signaling, Denvers, MA, USA). Cellular extracts were resolved in a 9% SDS-polyacrylamide gel and transferred to PVDF membranes (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated in Blue Loading Buffer containing 50 mM DTT (Cell Signaling, Danvers, MA) at 95°C for 5 min and resolved by SDS-PAGE ...
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bioRxiv - Genetics 2020Quote: ... cells were lysed under native conditions by homogenization in 1× cell lysis buffer (Cell Signaling) supplemented with Complete protease inhibitor tablet (Roche) ...
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bioRxiv - Physiology 2022Quote: ... Whole adipose tissue was homogenized in ice-cold RIPA buffer (Cell Signaling Technologies, cat# 9806) containing 2mM activated Na3VO4 and 1% protease inhibitor cocktail (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: The cells incubated under the indicated conditions were dispersed in lysis buffer (Cell Signaling; 9803) containing 20 mM Tris ...
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bioRxiv - Physiology 2022Quote: Western blotting was performed as previously described [26] using RIPA lysis buffer (Cell Signaling Technology) with added protease and phosphatase inhibitors (Cell Signaling Technology) ...
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bioRxiv - Developmental Biology 2023Quote: ... The pellets were resuspended in an equal volume of 2x RIPA buffer (Cell Signaling Technology) containing a 2x Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... minced and incubated with 5 ml 1x Red Blood Cell Lysis Buffer (Cell Signaling Technology) for 5 min with manual shaking ...
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bioRxiv - Genetics 2022Quote: ... the DNA Purification Buffers and Spin Columns for ChIP and CUT&RUN (Cell Signaling, #14209), with elution in 50 ul.
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bioRxiv - Biochemistry 2023Quote: ... after the supernatant was diluted 1:10 in 1× Cell Lysis Buffer (Cell Signaling #9803). Plates were read on an Epoch Microplate Spectrophotometer with Gen5 Software (Biotek Instruments) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Total cellular proteins were harvested by 1× RIPA lysis buffer (Cell Signaling Technology, MA, USA) containing phosphatase inhibitor and protease inhibitor ...
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bioRxiv - Immunology 2024Quote: ... Blocking was performed with plant-based protein blocking buffer (Cell Signaling Technology [CST], MA, USA) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... These tissue samples were washed extensively (6 times) with RBC lysis buffer (Cell Signaling Technology) until the soret absorption peak on the UV-visible spectra was almost negligible and the washes were colorless ...
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bioRxiv - Cancer Biology 2023Quote: ... before proteins were eluted into 150 μl 1x Blue Loading Buffer (7722S; Cell Signaling Technology) by boiling at 99 °C for 10 minutes.
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bioRxiv - Bioengineering 2023Quote: Cells were lysed in cell lysis buffer (Cell Signaling Technology, cat. no. 9803, Danvers, MA) supplemented with a protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested and lysed using RIPA buffer containing protease inhibitor (Cell Signaling Technology 9806S) and phosphatase inhibitor (PHOSS-RO Roche 4906845001) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells are lysed in 200 μL 1X Cell Lysis Buffer (catalogue number 9803, Cell Signaling). Protein lysate concentration is then determined using BCA protein assay kit (Catalog number 23225 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 50ul of Buffer 1 + 1X pA/G-MNase (Cell Signaling, cat. 57813) and incubated on ice for 1 hour and washed twice with Buffer 2 (0.05% w/v Saponin ...
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bioRxiv - Neuroscience 2023Quote: Proteins were extracted from day 40 neural organoids using RIPA buffer (Cell Signaling Technology, 9806) supplemented with protease and phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were immediately collected in 50μl of cell lysis buffer (Cell Signaling Technology) supplemented with Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: Hippocampal tissue was homogenized fresh after dissection using cell lysis buffer (Cell Signaling Technology, MA). Protein samples were separated using SDS-PAGE 10% precast gels (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: EOC cell lines were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, BioConcept) containing a proteinase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were prepared from 5x106 cells using cell lysis buffer (Cell Signaling Technology, 9803S) supplemented with protease-phosphatase inhibitor (ThermoFisher ...
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bioRxiv - Genomics 2024Quote: Cells were harvested by removal of media and addition of RIPA buffer (Cell Signaling Technology #9806 ...
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bioRxiv - Microbiology 2024Quote: ... Following incubation with a secondary mouse antibody (Cell signaling,1:1000 dilution in blocking buffer) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ChIP-PCR was performed using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Cell Signaling, Cat # 9005) by following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor lysates were prepared in Kontes tubes with disposable pestles using RIPA Buffer (Cell Signaling Technology) supplemented with phenylmethylsulphonyl fluoride (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were resuspended in 500 mL of FACS buffer with 0.5μg/mL DAPI (Cell Signaling Technology). The cell suspensions were analyzed using the FACS Fortessa (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: Cell lysates or mouse tissues were prepared using 1× cell lysis buffer (Cell Signaling Technology, USA) with 1 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Genomics 2020Quote: ... with each buffer supplemented with 1mM PMSF and 1x protease inhibitor cocktail (Cell Signaling Technologies, #5871).
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bioRxiv - Genetics 2019Quote: ... The agarose affinity gel was then washed three times with Cell Lysis Buffer (Cell Signaling Technology) and bound proteins eluted with NuPAGE LDS Sample Buffer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Flow cytometry enriched cell populations were incubated in lysis buffer (9803, Cell Signaling Technology, Boston, USA) on ice for 30 min and protein concentration measured by BCA assay (7780 ...
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bioRxiv - Neuroscience 2020Quote: ... after which the PBS was replaced with 200ul of RIPA buffer (Cell Signaling Technology, Danvers, MA) containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 293FT cells were incubated in the lysis buffer (Catalog No. #9803, Cell Signaling Technology, Danvers, MA) for half hour on ice ...
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bioRxiv - Cell Biology 2021Quote: Protein extracts from cells were obtained using RIPA 1X lysis buffer (#9806 Cell signaling, MA, USA) plus protease/phosphatase inhibitors (#P8340 and #P0044 ...
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bioRxiv - Cancer Biology 2021Quote: ... it was extracted with DNA Purification Buffers and Spin Columns (Cell Signaling Technologies, Cat. No 14209). Real-time PCR using different sets of primers (Supplementary Table S2 ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from cells were obtained using RIPA 1x lysis buffer (9806, Cell Signaling, MA, USA) plus protease/phosphatase inhibitors (#P8340/#P0044 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed in 500 μl of nuclear extraction buffer A (Cell Signaling Technology, Cat. 7006) on ice for 15 min and washed once with 500 μl of nuclear extraction buffer B (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2021Quote: ... Each pellet was resuspended in 4 mL of 1X Nuclei isolation buffer B (#7007, Cell Signaling) supplemented with 1 M DTT ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were incubated with kinase buffer containing 200 ng of human RB protein (Cell Signaling, 9303) and 200 µM ATP for 30 min at 37°C with gentle rocking ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with primary antibody in blocking buffer (polyclonal rabbit anti-HA antibody (Cell signaling, 1:100) overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated on ice for 15 min in RIPA lysis buffer (Cell Signaling, Danvers, MA) supplemented with Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... After 72hr of treatment cells were lysed with 1x Cell Lysis Buffer (9803, Cell Signaling Technology) according to the reagent protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... The cell pellet (2 × 107 cells) was suspended wit 2 mL of Lysis buffer (Cell Signaling) supplemented with Protease Inhibitor Cocktail Set III (Merck Millipore) ...