Labshake search
Citations for Cell Signaling Technology :
601 - 650 of 790 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed on ice at 1000 cells/µL lysis buffer according to the manufacturer’s protocol of the active RAS detection kit (Cat.#8821; Cell Signaling Technology). Total protein concentrations of samples were measured using a BCA protein-assay kit (Cat.#23225 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins samples were processed in SDS free lysis buffer for the assessment of Ras-GTP level using Active Ras Detection Kit (Cell Signaling) by following manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2022Quote: ... antigens were retrieved by boiling slides in citrate buffer (pH 6.0) for 1 min and pSMAD1/5 Ab (Cell Signaling, 41D10) was added overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: Cell lysates for protein analysis were prepared by scraping cells in 1x Cell Lysis Buffer (Cell Signaling Technology, Danvers, MA, USA) containing 1x protease/phosphatase inhibitor (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
-
bioRxiv - Systems Biology 2022Quote: ... cells were lysed in RIPA buffer and analyzed by SDS-PAGE followed by western blotting with antibodies to phospho and total Smad3 (Cell Signaling). The blots were quantified using an Odyssey fluorescent imaging system.
-
bioRxiv - Cancer Biology 2022Quote: ... rinsed in phosphate buffer 0.2 % Triton for 15 min and incubated with rabbit anti-P-cad (1:50; Cell Signaling; 2130S) or anti-pSrc (pY419 ...
-
bioRxiv - Cancer Biology 2022Quote: Cells with stable expression of FLAG-tagged human PDE6D were lysed in 1X cell lysis buffer (#9803, Cell Signaling, Danvers, MA) and incubated with anti-FLAG M2 Affinity Gel (A2220 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Briefly, sections were incubated with primary antibody (rabbit anit-PH3, 1:100 dilution in immunofluorescence buffer,) (Cell Signaling, Danvers, MA, 9701S) overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400 ...
-
bioRxiv - Microbiology 2020Quote: ... on ice for 15 min and washed once with 500 μl of nuclear extraction buffer B (Cell Signaling Technology, Cat. 7007), and then treated with 0.5 μL of micrococcal nuclease (Cell Signaling Technology ...
-
bioRxiv - Molecular Biology 2022Quote: ... The remaining 4.9 mg of digests were re-suspended in 1.4 mL of immunoaffinity purification buffer (Cell Signaling Technology, Danvers, MA) containing 50 mM 4-morpholinepropanesulfonic acid (MOPS)/sodium hydroxide ...
-
bioRxiv - Physiology 2022Quote: Liver samples (∼50 mg) were homogenized on ice for 60 seconds in 10X (v/w) RIPA Buffer (Cell Signaling, Danvers, MA) with protease and phosphatase inhibitors (Boston BioProducts ...
-
bioRxiv - Immunology 2019Quote: ... The concentrated supernatant was mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500; Cell Signaling) or mature IL-1β (Asp116 1:500 ...
-
bioRxiv - Cancer Biology 2019Quote: ... samples were resuspended in 750 uL 1X IAP Buffer (50 mM MOPS pH 7.2, 10 mM sodium phosphate, 50 mM NaCl from Cell Signaling Technology) using vortex and brief bath sonication ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were then centrifuged again and removed of supernatant before resuspension in isolation buffer (calcium and magnesium-free 1xPBS, 0.1% (w/v) bovine serum albumin (BSA) (Cell Signaling Technology), 2mM EDTA ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
-
bioRxiv - Molecular Biology 2021Quote: ... ChIP was performed using an amount of BEL-ChIP nuclear extract corresponding to 3.4 μg of input genomic DNA in 250 μL of ChIP Buffer + 1X PIC (SimpleChIP Kit #9003, Cell Signaling Technology). Antibody was added according to the vendor’s specifications and incubated overnight with end-over-end mixing at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... The drying product was dissolved in 100 μl PBS and mixed with SDS loading buffer for western blotting analysis with antibodies for detection of activated caspase-1 (D5782 1:500, Cell Signaling), mature IL-1β (Asp116 1:500 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Associated GTP-bound forms of Rho were released with SDS–polyacrylamide gel electrophoresis loading buffer and analyzed by Western blot analysis using a monoclonal antibody against RhoA (Cat #2117, Cell Signaling). Normalization took place based on the total RhoA levels present in the whole cell lysate of the same samples.
-
bioRxiv - Immunology 2019Quote: ... NGP overexpressing THP-1 cells were lysed by 1X sample buffer and then western blotting was performed by myc antibody (Cell Signaling). For NGP western blot ...
-
bioRxiv - Immunology 2019Quote: Samples were washed twice in ice cold HBS and then lysed in 1000ml lysis buffer for 30 minutes at 4°C with end over end mixing (Cell Signaling Lysis Buffer 20mM Tris-HCL pH7.5 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
-
bioRxiv - Neuroscience 2022Quote: ... 20 third instar larvae per genotype were flash frozen in liquid nitrogen and homogenized in 100 μL RIPA buffer (Cell Signaling) supplemented with cOmplete protease inhibitor cocktail (Roche ...
-
bioRxiv - Cancer Biology 2022Quote: ... harvested at 50-80% confluence and lysed by sonication in RIPA buffer (Cell Signaling Technology; 20 mM Tris-HCl (pH 7.5), 150 mM NaCl ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were reblocked with PBS Blocking Buffer and reprobed overnight at 4°C with anti-Akt antibody (Cell Signaling Technology #9272) diluted 1:1,000 in PBS antibody buffer ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
-
bioRxiv - Physiology 2022Quote: ... Isolated islets and MIN-6 cells were mechanically homogenized in ice cold RIPA lysis buffer (Cell Signaling Technology, Danvers, MA, USA) supplemented with 1x protease inhibitors cocktail (Sigma ...
-
bioRxiv - Biophysics 2023Quote: ... The blots were probed with an anti-BRD9 rabbit monoclonal antibody (Cell signaling technology 48306, 1:500 in the blocking buffer) and an anti-β actin mouse monoclonal antibody (Santa Cruz Biotechnology sc-47778 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were diluted in blocking buffer and applied for 1 hour at RT: rabbit anti-p53 (Cell Signaling Technology, 2527) at 1:200 dilution ...
-
bioRxiv - Immunology 2023Quote: ... stimulated T cells were fixed with the Foxp3/TF staining buffer set (eBioscience) for 30 min at 4°C and stained intranuclearly with rabbit polyclonal anti-mouse HIF-1α (Cell Signaling) o/n in permeabilization buffer (eBioscience) ...
-
bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803 ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue was rapidly weighed and homogenized with a pestle homogenizer in the following solution: 1x Cell Lysis Buffer (Cell Signaling, 9803S), 0.5% (v/v ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membranes were then incubated in the same buffer with anti-TAZ and anti-β -actin antibody (#8418, #5125, Cell Signaling) or anti-IHH antibody (#13388-1-AP ...
-
bioRxiv - Immunology 2023Quote: ... Tissues were then stained with the following fluorophore-conjugated anti-mouse antibodies for (1:100, 1.5 hr, room temperature, gentle agitation) in blocking buffer: Iba-1 (78060S, Cell Signaling Technology), F4/80 (123122 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were lysed by ice-cold RIPA buffer (150mM NaCl, 1% NP40, 0.1% SDS and 50mM Tris, PH7.4) containing protease and phosphatase inhibitor (Cell Signaling Technologies; 5872) for 20 minutes ...
-
bioRxiv - Cancer Biology 2023Quote: ... The DNA fragments were purified using the DNA purification buffers and spin columns (ChIP, CUT&RUN, Cell Signaling Technology, cat# 14209) and SYBR Green-based real-time PCR was conducted ...
-
bioRxiv - Physiology 2023Quote: ... Tibialis anterior (TA) and EDL were homogenized using an MP homogenizer in RIPA buffer (#9806, Cell Signaling Technologies Inc., Danvers, MA) supplemented with a protease and phosphatase inhibitor cocktail (Halt ...
-
bioRxiv - Microbiology 2023Quote: Samples used for measuring HMGB1 protein levels in the stool and colonic mucus were homogenized in cell lysis buffer (Cell Signaling) containing complete protease inhibitor (Roche ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were then diluted in three volumes of flow cytometry buffer (1x PBS, 25 mM HEPES pH 7.4, 1% bovine serum albumin [Cell Signaling Technology #9998] ...
-
bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membranes at 100 volts for 90 minutes in cold 1X transfer buffer (Cell Signaling Technology, 12539S). Membranes were blocked with 5% (w/v ...
-
bioRxiv - Neuroscience 2024Quote: ... Staining with anti-Pu.1 antibody in 1X Permeabilization buffer (microglia marker 1:50, Pu.1-AlexaFluor 647, 9G7 Cell Signaling) was performed for 40 min ...
-
bioRxiv - Neuroscience 2024Quote: ... Chromatin was sheared using a Covaris Sonicator for 20 minutes in sonication buffer (SimpleChIP Plus Sonication Kit, Cell Signaling Technology, #57976) to obtain fragments in the range of 200-500 bp ...
-
Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Clones with successful deletions based on the PCR result were then analyzed using Western blots with antibodies against FOS or EGR1 and pERK antibody (Cell Signaling Technology) to identify clones that lack expression of the respective TFs and still induce MAPK signaling when the RAF1-CR3 kinase domain is activated with 4OHT ...
-
bioRxiv - Cell Biology 2020Quote: ... the pellet was washed with PBS (–) and incubated on ice for 5 min in 0.1 ml cell lysis buffer (Cell Signaling Technology, Inc.). In the other conditions ...
-
bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
-
bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
-
bioRxiv - Genetics 2019Quote: ... The culture medium was then replaced with medium containing 50 mM biotin and cultured for a further 24 hours when the cells were lysed using RIPA Buffer (Cell Signaling Technology) with 1 µM phenylmethylsulfonyl fluoride (PMSF ...