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Citations for Cell Signaling Technology :
301 - 335 of 335 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Bioengineering 2020Quote: ... each gel was rinsed briefly in deionized water and then stored for >12 hours in 1X tris-buffered saline solution with Tween® (TBST, prepared from Cell Signaling Technology 9997S 10X stock and MilliQ ultrapure water ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody binding was directed against eGFP using a solution containing a 1/4000 dilution of a mouse anti-GFP 4B10 antibody (Cell Signaling Technology, USA). For all VLPs ...
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bioRxiv - Genetics 2020Quote: ... 500 μl of PI (Propidium Iodide) /RNase solution was added and incubated with the cells for 20 min at room temperature (Cell Signaling Technology, 4087). The cell suspension was then applied onto FACS tubes with cell strainer cap (Corning ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Normalization was carried out on the control group and related to the corresponding ß-actin (monoclonal mouse, Cat. No. #3700, dilution 1:2000 in blocking solution, Cell Signaling Technology and monoclonal rabbit ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Molecular Biology 2024Quote: ... brains were stained with primary antibody (NC82) diluted in antibody solution (970µL of PBST, 30µL NGS, 1µL rabbit anti-GFP (Cell Signaling Technology, Cat # 2956S, 1:1000), 50 µL mouse anti-DLG (1:20) ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were saturated with the blocking solution followed by a 1hr at 37°C incubation with the primary antibodies: Bcl-xL (Cell Signaling #2764, 1/1000) and IP3R C-terminal (abcam #ab190239 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were filtered and incubated in a 1:500 solution of mouse anti-HA antibody covalently bound to Alexa 647 (Cell Signaling 6E2, product #3444). Cells were incubated with the antibody by shaking vigorously for 15-30 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Membranes were incubated for 1 hour at room temperature with horseradish peroxidase-conjugated horse anti-mouse IgG solution (1:2500, Cell Signaling, Cat. No. 7074P2) diluted in 1% non-fat milk/TBST ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then blocked in 2% BSA-PBS solution for 1 hr at room temperature and then incubated with a primary antibody against β-catenin (1:100; Cell signaling technology, #9562) diluted in 2% BSA-PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies, Cat. # 13174) and an anti- rabbit HRP secondary antibody (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then incubated overnight at 4 °C with primary antibodies diluted in the blocking solution: anti-TRKB (1:500, Cell Signaling Technology, Cat# 4607S) and anti-GAPDH (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT#: 14782S, EZH2 1:250-1:500 Cell Signaling Technologies CAT# ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in a solution of rabbit anti-c-fos primary antibody (1:1000 dilution, 2250T Rabbit mAb /74620 Mouse mAb, Cell Signaling Technology, Danvers, MA, USA) or mouse anti-TPH2 primary antibody (1:100 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... The fixed cells were centrifuged and the pellets obtained were collected in flow cytometry tubes and stained with preformulated PI/RNase solution (Cell Signaling Technology, catalog number: 4087s) for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... every sixth section of the LPB was incubated in a primary antibody solution containing rabbit anti HA-Tag (C29F4) monoclonal antibody (1:1000; 3724s, Cell Signaling Technologies, Danvers, MA, USA) overnight followed by incubation in a solution containing a secondary Alexa-Fluor Plus 488 donkey anti-rabbit antibody (1:3000 ...
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bioRxiv - Systems Biology 2022Quote: ... an Odyssey blocking buffer solution containing 10% OptiPrep™ and 1:2000 Alexa 488-conjugated anti-phospho-histone H3 (S10) antibody (pH3) (clone D2C8, Cell Signaling Technology, Danvers, MA) to label M-phase cells and 2 µg/ml Hoechst 33342 to stain all nuclei ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated overnight at 4°C in a primary antibody solution consisting of rabbit-anti-pSMAD1/5/9 (1:600, Cell Signaling Technology #13820 Lot 3) and goat-anti-SOX2 (1:400 ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5% BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology ...
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bioRxiv - Neuroscience 2024Quote: ... slices were moved to the primary antibody solution containing one or more of the following antibodies in blocking solution and were left there overnight: rabbit-anti-c-Fos (1:1000; Cell Signaling Technology, 2250s, Leiden, Netherlands), chicken-anti-GFP(1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were placed overnight at 4°C in a solution containing 2% donkey serum and primary antibodies for post-synaptic density protein 95 (PSD95; rabbit host, 1:100, Cell Signaling Technology, Danvers, MA, product #2507, lot 2), vesicular glutamate transporter 1 (VGLUT1 ...
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bioRxiv - Bioengineering 2023Quote: ... washed three times for 10 min in PBS and incubated for 1 h in blocking solution (5% normal goat serum, Cell Signaling Technology #5425, 0,3% Triton X-100 in PBS) at room temperature ...