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Citations for Cell Signaling Technology :
251 - 300 of 335 citations for Corning Hygromycin B Solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed in water and boiled in 1X Citrate Buffer unmasking solution (Cell Signaling cat# 14746S). Tissue sections were then incubated in a 3% H202 solution for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... Solution was then extracted and purified using DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, Cell Signaling #14209). DNA samples were then used for qPCR or sequencing.
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then incubated overnight in rabbit anti-ΔFosB (1:2000 in blocking solution, Cell Signaling, Danvers, MA) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Blocking buffer was removed and replaced with primary antibody solution (rabbit α-HA (C29F4, 1:1000, Cell Signaling Technology) and mouse α-HSP70 (5A5 ...
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bioRxiv - Developmental Biology 2024Quote: ... the blocking solution was drained and replaced with primary antibody: Cleaved Caspase-3 (1:250, Cell Signaling cat#9661T), or αPhospho-histone H3 (Ser10 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated overnight at 4 °C with primary antibody in the same solution used for blocking (Cell Signaling Technology rabbit antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were labeled with a one-step staining solution containing anti-RelA (1:400, Cell Signaling Technology, #8242), Nano-secondary AF568 (Chromotek ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were then incubated overnight in blocking solution with antibodies recognizing CD45 (Cell Signaling cat. no 70257S; 1:200) or CD3 (Abcam cat ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were diluted in the blocking solution at the following concentrations: H2K9ac 1:400 (9649, Cell Signaling Technologies), Lamin A/C 1:10,000 (4777 ...
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bioRxiv - Microbiology 2024Quote: ... Actin is also blotted by primary antibody solution of beta-Actin 8H10D10 antibodies (produced in mouse) from Cell signaling in blocking solution (1:10000) ...
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bioRxiv - Cell Biology 2021Quote: ... after each step samples were washed 3x with PBS+/+ and mounted in ProlongGold + DAPI solution (Cell Signaling Technologies, CST 8961S). Z-stack image acquisition was performed on a confocal laser scanning microscope (Zeiss LSM880 ...
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bioRxiv - Microbiology 2023Quote: ... A non-competitor solution was prepared by diluting anti-myc Alexa 647 (clone 9B11, 1/250 dilution, Cell Signaling Technology) in PBS-BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Chromatin solutions were incubated with 10 ug of anti-HIF1α (Cayman, Rabbit, #10006421) antibody or anti-rabbit IgG (Cell Signaling #2729) overnight at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibody solution contained 1:500 Alexa Fluor 488 antibody goat anti-rabbit (4412S, Cell Signaling Technologies, Danvers, MA, United States) with 0.1 M PBS ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibodies were diluted in blocking solution overnight at 4°C: phos-NFkB p65 (Ser536) (93H1) Rabbit mAb (1:1000) (Cell Signaling), phos-Stat3 (Tyr705 ...
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bioRxiv - Genetics 2022Quote: ... Beads were then resuspended in 250 μl blocking solution and 2 μg antibody (RAD21, Abcam ab992; CTCF, Cell Signaling Technology 3418) was added ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight in rabbit anti-c-Fos primary antibody (1:200 in blocking solution, Cell Signaling Technologies, Ab #2250). Following overnight incubation in primary antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was then transferred to a secondary antibody solution containing a 1:5000 dilution of α-mouse HRP (Cell Signaling) for one hour of shaking at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Then the sections were incubated with primary antibody of interest in the blockage solution (c-fos,1:200, Abcam/ab208942; NFκB-p65, 1:200, Cell Signaling Technology/8242 ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... rinsed in 1x TBS-T (137 mM sodium chloride, 20 mM Tris, 0.1% Tween-20) (diluted from 10x solution from Cell Signaling Technology), and placed on top of a stack of 2 sheets of 3MM Chr chromatography paper (Whatman ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h and incubated overnight at 4°C with the corresponding primary antibody diluted in blocking solution: Anti-Caspase 3 (rabbit, 1:100, Cell Signaling). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was rapidly weighed and homogenized with a pestle homogenizer in the following solution: 1x Cell Lysis Buffer (Cell Signaling, 9803S), 0.5% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then incubated overnight in the saturation solution containing the primary antibody (rabbit anti c-Fos (1/1500, Cell Signaling), sheep anti-BrdU (1/2500 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743 ...
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bioRxiv - Biophysics 2024Quote: ... cells were washed 3X in PBS and blocked for 1 h at RT with 5% BSA solution containing 5% normal goat serum (Cell Signaling) and 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then washed with CyTOF permeabilization buffer and the barcoded samples were combined before being incubated with antibodies in a solution containing 10% NGS (Cell Signaling #5425). Cells were then washed with CyTOF staining buffer and fixed with 10% formalin (Merck #F5554 ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... They were rinsed with 1x PBS again and incubated with 50 μL of blocking solution (1x PBS, 0.5% goat serum) with 1:300 anti-rabbit mTOR (Cell Signaling Technology, #2983S) for 1 h at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of each substrate solution was added to 1 mL of PBS or RIPA lysis buffer (Cell Signaling Technology, Danvers, MA) for NLuc experiments ...
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bioRxiv - Bioengineering 2022Quote: ... We cultured tricultures for 7 days and subsequently stained them with CD31 and phalloidin (7 μL per 1000 μL solution) or cytokeratin 18 (CK18; 1:250, Cell Signaling 24E10) using the protocol described above ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody solution contained a 1/1000 dilution of a monoclonal rabbit anti-HA antibody (C29F4, Cell Signaling Technology, Danvers, USA) in Tris-buffered saline (TBS ...
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bioRxiv - Bioengineering 2022Quote: ... the membrane was incubated overnight at 4°C or with primary antibody solution (1:1000 dilution for anti-Alix [E4T7U, Cell Signaling Technology] ...
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bioRxiv - Neuroscience 2022Quote: ... for 1h at room temperature and incubated over night at 4°C with the following antibodies diluted in blocking solution: anti-RSK2 (1/1000, Cell Signaling Technology); anti-RPS6 (1/1000 ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution using the following concentrations: 1:1000 anti β-actin (13E5) rabbit primary antibody (#4970 from Cell Signaling), 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara) ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Pathology 2023Quote: ... They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242; Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were fixed in ice-cold 70% ethanol for at least 30 minutes and were then labeled with propidium iodide (PI)/RNase staining solution (#4087, Cell Signaling Technology) and further incubated for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Digestion was stopped by adding 1× stop buffer (digitonin solution, RNase A, and 50 pg S. cerevisiae spike-in DNA (Cell Signaling Technology) for normalization ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were rinsed with pre-warmed 1x PBS and fixed with 1x fixative solution provided by senescence beta-galactosidase staining kit (9860, Cell Signaling Technology) for 15 minutes ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed in TBST and incubated with HRP - conjugated anti-rabbit IgG at 1:2000 dilution in blocking solution (#7074, Cell Signaling Technology). Bands were revealed with ECL Prime reagent (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were cut for different antibodies depending on protein size and placed into the appropriate antibody solution made in TBST (CBS 1:1000 Cell Signaling Technologies CAT# ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... cells were incubated in the blocking solution with the HRP linked anti-mouse or anti-rabbit secondary antibody (respectively #7076 or #7074; Cell Signaling Technology) at 1:10,000 dilution (1 hr at RT in the dark) ...
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bioRxiv - Genomics 2024Quote: ... Separate PCLS were fixed for 30 minutes in the fixative solution from the β-galactosidase staining kit (Cell Signaling Technology Cat#9860) and then washed in PBS.
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...