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Citations for Cell Signaling Technology :
2701 - 2750 of 2787 citations for 6 Phenylamino 2 3 dihydro 1H indolizin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Plant Biology 2024Quote: ... The membranes were incubated overnight in agitation at 4°C with Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370; Cell Signaling Technology) in a dilution 1:1000 with PFBS ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated using a 10% acrylamide SDS-PAGE gel and immunoblotted with anti-phospho-p44/42 MAPK (Erk1/2) (anti-pMAPK; Cell Signaling Technology).
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of SARS-CoV-2 nucleocapsid protein (NP) (E8R1L) fragmented mouse monoclonal antibody f-mAb (Cell Signaling Technology Inc., Massachusetts, USA) are introduced to the sensor surface after diluting in PBS and incubated for 2 hours at room temperature ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was blotted with 1:1,000 dilutions of antibodies for phospho-p44/42 MAPK (phospho-Erk1/2; Cell Signaling Technology #9101), p44/42 MAPK (Erk1/2 ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µL of samples from each fraction were lysed with 5 µL 1:1 mixture of RIPA buffer (Cell Signaling Technology, Danvers, MA. Cat #: 9806S) and protease inhibitor (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole embryos were fixed in 4% paraformaldehyde overnight and stained with anti-phospho-Smad1/5/9 Rabbit monoclonal antibody (Cell Signaling Technology #13820S; 1:500), anti-phospho-H3 Rabbit monoclonal antibody (Cell Signaling Technology #9701S ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were then blocked with 5% nonfat milk at room temperature for 1 hour and then probed with primary antibodies (phospho-p44/42 MAPK, Cell Signaling 9101, 1:4000, dilution) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody dilutions were prepared in 5% BSA-TBST and incubated overnight with gentle agitation at the following dilutions phospho-AKT T308 1:2000 (Cell Signaling Technology Cat# 13038, RRID:AB_2629447), AKT 1:1000 (Cell Signaling Technology Cat# 9272 ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was washed with TBST and incubated in TBST + 5% (w/v) nonfat milk and anti-rabbit IgG HRP-linked antibody (Cell Signaling catalog #7074, 1:1000) for 1 hour then washed in TBST ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed four times for 5 min each and treated with 1:1000 diluted Alexa Fluor® conjugated sec antibody (Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The membranes were blocked with 5% BSA at room temperature for 30 min and incubated with anti-eNOS (clone: D9A5L, Cell Signaling Technology, MA, USA, 1:1,000) and anti-Ser177 p-eNOS (clone ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were carried out on the remaining sample overnight at 4 °C with rotation with 5 uL of antibody (Cell Signaling Technology D31H2 anti-CTCF, 3418S). 50uL of Protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:100), Rat anti-GFP monoclonal antibody (nacalai tesque, GF090R, diluted 1:1000) and rabbit anti-pSmad1/5/9 antibody (Cell Signaling Technology, #13820, diluted 1:100). After the primary antibody incubation ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-phospho-SAPK/JNK antibody (#9251) and anti-phospho-p44/42 MAPK (Erk1/2) antibody (#9101) were obtained from Cell Signaling Technology (MA, USA). The PPARγ inhibitor ...
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bioRxiv - Biochemistry 2021Quote: Cleaved-caspase-3 immunostaining was performed on 2 µm thick formalin-fixed paraffin sections in a 1:200 dilution according to the manufacture’s protocol (Cell Signaling Technology, Danvers, MA, USA). Pictures were taken on a slide scanner SCN4000 in a 20× magnification.