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Citations for Cell Signaling Technology :
2501 - 2550 of 2787 citations for 6 Phenylamino 2 3 dihydro 1H indolizin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Proteins were separated by electrophoresis on 10% or 12% SDS-polyacrylamide gels and transferred to PVDF membranes that were probed with anti-pSmad1/5/8 (Cell Signaling 9511S), anti-BMP4 (Santa Cruz sc12721) ...
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bioRxiv - Cell Biology 2019Quote: ... Filters were incubated at 4 °C with primary antiserum diluted in 5% milk TBST as follows: FLAG antiserum (1:1000, Cell Signaling Technology), α-tubulin antiserum (1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were diluted in either 5% goat serum/PBS-T or SignalStain Boost IHC Detection Reagent (8114S, Cell Signaling Technology (CST)) ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... coupled with the desired antibodies (5 ug each of H3K27me3 {Millipore 07-449}, H3K4me3 {Millipore 04-745} and H2AUb {Cell Signaling 8240S}) ...
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bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C with the following primary antibodies diluted 1:1000 in 5% BSA/TBST: anti-pro-IL-1β (#12703, Cell Signaling Technology) and anti-NLRP3 (AG-20B-0014-C100 ...
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bioRxiv - Immunology 2020Quote: ... cells were cytospun 5’ at 500 rpm and senescence-associated beta-glalactosidase activity measured as recommended by the manufacturer (Cell Signaling Technology) on a Zeiss-Axio inverted phase-contrast microscope ...
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bioRxiv - Cell Biology 2021Quote: ... blocked with 5% BSA/PBS (1 h at room temperature) and stained with the following primary antibodies to: vimentin (1:500; Cell Signaling, #5741), Ki67 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... NCI-H522 and NCI-H1299 cells were treated with 5 μg/mL Poly I:C sodium salt (Cell Signaling Technologies Cat No. 61401) for 6 and 12 hours respectively.
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bioRxiv - Developmental Biology 2022Quote: ... Magnetic beads were allowed to bind with 5 μg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) by incubating for 8 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were then washed three times for 5 min with PB and mounted with a cover glass using ProLong (Cell Signaling Technology).
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Genomics 2023Quote: ... Membranes were washed in TBST and incubated 1 hour at room temperature with secondary antibody diluted 1:5000 in 5% milk / TBST [anti-rabbit IgG HRP (Cell Signaling 7074S)] ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... the fixed cells were blocked in PBS containing 5% bovine serum albumin (BSA) and then incubated with mouse anti-HA antibody (6E2, Cell signaling Technology) with 1:800 dilution overnight at 4°C ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... Astrocyte activation to a neurotoxic phenotype was induced in cells at 4-5 DIV by incubation with TNFα (25 ng/ml; Cell Signaling Technology), IL-1α (3 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... + 5% milk and then incubated overnight at 4°C with the following primary antibodies in TBS+5% milk: anti-RPB1 (1:1000; Cell Signaling, 14958), anti-EZH2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Systems Biology 2024Quote: ... anti-MHC Class I (clone EMR8-5, cat#: 88274) and anti-PSMB9 (clone E7J1L, cat#: 87667) were purchased from Cell Signaling Technology. Puromycin (cat# ...
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bioRxiv - Immunology 2023Quote: ... blocked with TBST (1x Tris buffered saline with 0.1% Tween 20) supplemented with 5% (wt/vol) BSA for 1 hr and blotted with PU.1 (9G7) (1:1000, Cell Signaling #2258) at 4°C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then cut and incubated with different primary antibodies diluted in 5% milk at 4°C overnight: αH3K27me3 (Cell Signaling C36B11) was used at 1:5000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... were incubated overnight (16 hours) in 1X TBST supplemented with either 5% non-fat dry milk or bovine serum albumin (#9997, #9998, Cell Signaling Technology) at 4○C with constant rocking according to the manufacturer’s recommendation ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in TBST plus 5% non-fat milk and then incubated with the following primary antibodies: phospho-S6 (S235/236; 1:2000, Cell Signaling, #4858), S6 (1:2000 ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were added overnight in 5% milk in TBS + 0.1% tween-20 (TBST): huntingtin (Clone D7F7, Cell Signaling 5656, 1:1,000), beta-actin (Clone 13E5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk and incubated with primary antibodies overnight and incubated with anti-rabbit secondary antibody from Cell Signaling Technologies for one hour after which blots were developed using Bio-Rad ChemiDoc™ Imaging Systems ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000, Cell Signaling Technology, 67B9), rabbit anti- Pten (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... permeabilised with 90% (v/v) methanol and then intracellularly stained with anti-phospho-p44/42 MAPK (Erk1/2; Cell Signaling) for 30 min at room temperature and then with secondary antibodies against rabbit IgG (H+L ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: The kinase inhibitors used were against cSrc (Dasatinib 100nM), AKT (Perifosine 10μM), ERK1/2 (U0126, 10μM) (0952, 14240 and 9903, respectively, Cell Signaling Technologies) and pNaKtide (1μM ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4°C with the rabbit anti-phospho-p44/42 MAPK (for pErk1/2) (Cell Signaling Technology) at 1:250 ...
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bioRxiv - Cancer Biology 2019Quote: ... Other primary antibodies used are anti–Phospho-p44/42 MAPK (phosphorylated ERK1/2, Thr202/Tyr204, 197G2, Cell Signaling Technology #4377), anti-p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated overnight with rabbit polyclonal primary antibodies against phospho-p44/42 MAPK (Erk1/2) (Cell Signaling, 1:2000) at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were used: rabbit anti-Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (4370, 1:100, Cell Signaling), rabbit anti-Phospho-Drosophila p70 S6 Kinase (Thr398 ...
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bioRxiv - Physiology 2022Quote: ... anti-p44/42 MAPK (Erk1/2) (#4695) and anti-Acetyl-α-Tubulin (Lys40) (#5335) antibodies were from Cell Signaling Technology. Anti-Lamin A/C (sc-20681) ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibodies used for immunofluorescence staining were as follows: anti-Phospho Myosin Light Chain 2 (Cell Signaling Technology, #3671), anti-ZO-1 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... and the plate was extensively washed with TBST + 100 mM EDTA and a 1:8,000 dilution of primary antibody (p-MEK1/2 (S217/S221) Rabbit Antibody (Cell Signaling)) was added to the plate and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies against the following proteins were used in the indicated dilutions: ERK1/2 (#4695, RRID:AB_390779, Cell Signaling Technology, 1:50), JUN (#9165 ...
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bioRxiv - Cancer Biology 2020Quote: ... phospho-Erk1/2 T202/Y404 (4370) 1:2000 and Erk1/1 (4695) 1:2000-1:5000 (all from Cell Signaling); and Tubulin (T5168 ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg of fusion proteins and 8 μg of FLAG-PKD1-CA were incubated in kinase buffer (Cell Signaling Technology) for 30 min at 30°C in the presence of 200 μM ATP ...
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bioRxiv - Cancer Biology 2021Quote: ... Zebrafish embryos were stained with primary antibodies directed against Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (4370S, Cell Signaling) at 1:200 ...
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bioRxiv - Plant Biology 2022Quote: ... and probed with anti-phospho-p44/42 mitogen-activated protein kinase (Erk1/2) (Thr202/Tyr204) (Cell Signaling; 1:1,000 dilution), anti-MBP (New England Biolab ...
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bioRxiv - Cell Biology 2022Quote: ... phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370) and pS473-Akt (#4060) antibodies were from Cell Signaling Technology (CST). Further antibodies used were against GST (sc-138 ...
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bioRxiv - Plant Biology 2022Quote: ... The primary antibody was anti-p-ERK (anti-pERK MAPK [Phospho-p44/p42 MAPK, ERK1/2, Thr202/Tyr204, D13.14.4E], Cell Signaling Technology) that specifically recognizes the [T-X-Y] MAPK activation motif ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining supernatants were subjected to immunoprecipitation by incubation with 2 μl of anti-mTOR antibody (#2983, Cell Signaling Technology) for 3 hours (4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Constant volumes of sample were incubated overnight with 2 μg α-BRD4 antibody (Cell Signaling, Danvers, MA, USA, Cat. # 13440) or a nonspecific isotype control (LSBio ...
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: i) Phospho-Myosin Light Chain 2 (Ser19) (1:50, Rabbit, Cell Signaling Technology #3671S) ii ...