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Citations for Cell Signaling Technology :
201 - 250 of 296 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... Tendons were homogenized in radioimmunoprecipitation assay (RIPA) buffer with protease/phosphatase inhibitors (Cell Signaling Technology [CST] #58725) using 0.5-mm zirconium oxide beads and a Bullet Blender Gold Cell Disrupter (Next Advance Inc.) ...
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bioRxiv - Developmental Biology 2020Quote: This assay was performed using a commercially available senescence β-Galactosidase staining detection kit (Cell Signaling, #9860). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: The CUT&RUN experiments were performed using the Cut and Run Assay Kit (Cell Signaling Technology, 86652S). EpCAM+ epithelial cells from c57Bl/6 E17.5 mouse lungs were isolated and ∼100,000 cells were bound to concanavalin A beads and then incubated overnight with antibodies for PRDM16 (Sheep anti-PRDM16 R&D AF6295 ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin immunoprecipitation (ChIP) assay was performed by using SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology, #9003) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was mounted on Superfrost Plus precleaned slides (Fisher; catalog # 12-550-15) followed by cover-slipping with Prolong Gold antifade mounting media (Cell Signaling Technology, catalog # 9071S) and sealed with nail polish.
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bioRxiv - Immunology 2021Quote: ChIP assays were performed using a SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... caspase-3 activity was determined by Caspase-3 Activity Assay Kit (Cell Signaling Technology, Inc., Danvers, MA, USA). Primary cortical neurons were seeded onto 96-well plates ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ChIP assay was conducted using the Simple ChIP(R) Enzymatic Chromatin IP Kit purchased from Cell Signaling Technology following the manufacturer’s instruction ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... MAPK activity assay was performed by immunoblotting with anti-phospho p44/42 MAP kinase antibody (Cell Signaling Technology). For gene expression assays ...
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bioRxiv - Cell Biology 2022Quote: Exosomes isolated by ultracentrifugation were lysed in equal volume of radio-immunoprecipitation assay (RIPA) buffer (Cell Signaling Technology) containing protease inhibitor cocktail (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunofluorescence assays were performed with the following primary antibodies: anti-Trx-1 (1:50, Cat# 24295, Cell Signaling) and anti-eGFP (1:400 ...
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bioRxiv - Cell Biology 2021Quote: Cells cultured on 6-well plates were stained using SA-β-gal activity assay kit (Cell Signaling Technology) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ChIP assays were conducted with the use of SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads; Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and Rabbit (DA1E) mAb IgG XP® Isotype Control (15 μL/assay, #66362; Cell Signaling Technology, Danvers, MA). DNA obtained from the CUT&RUN assay was amplified using SYBR Green qRT-PCR with the StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The immunocytochemistry-based assay was performed with Senescence β-Galactosidase Staining Kit (Cell Signaling Technology, catalog number: 9860) according to the manufacturer’s guidelines ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ChIP assay was conducted using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling Technology, Danvers, Massachusetts, USA) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies for western blots and co-immunoprecipitation assays were anti-phospho-p44/42 MAPK (Cell Signaling, Technology, 9101), anti-p44/42 MAPK (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... ChIP assays were performed using a SimpleChIP Enzymatic Chromatin IP kit brought from Cell Signaling Technology (9003, USA). HeLa cells were treated with estradiol (10-5M ...
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bioRxiv - Cancer Biology 2023Quote: ChIP assays were performed using the SimpleChIP plus Enzymatic Chromatin IP kit (9005S; Cell Signaling Technology, Danvers, MA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: G6PD enzyme activity was determined using the Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit (12581S, Cell Signaling Technology) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 90,000 −120,000 cells were used per condition to perform CUT&RUN using CUT&RUN assay kit (86652S, Cell signaling) with an anti zebrafish Pu.1 antibody (GTX128266 ...
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bioRxiv - Developmental Biology 2020Quote: ... The ChIP assay was performed using the SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Beverly, MA) following the manufacturer’s protocol with some modifications(56) ...
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bioRxiv - Cancer Biology 2021Quote: CUT&RUN for H3K9ac in A2058 cells was carried out using the CUT&RUN Assay Kit (Cell Signaling Technology), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: Chromatin immunoprecipitation assays were performed using the SimpleChIP Enzymatic Chromatin IP Kit with magnetic beads (Cell Signaling Technology, MA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: The ChIP assays were performed using the Simple Chip Enzymatic Chromatin IP kit (Cell Signaling Technology, Danvers, MA, USA) according to the manufacturer’s instructions.GECs were crosslinked with EBM-2 containing 1% formaldehyde for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP assay was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (9003, Cell Signaling Technology, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin immunoprecipitation assays were performed using SimpleChIP® Enzymatic Chromatin IP Kits (Magnetic Beads) (#9003, Cell Signaling, Ozyme, France). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: Protein samples were collected and lysed using a radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Cell Signaling Technology). Lysates were quantified by Bradford assay (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: The SA-β-gal assay was performed with a Senescence beta-galactosidase staining kit (Cell Signaling Technology, Danvers, Massachusetts). β-galactosidase staining solution and the fixative solution were arranged with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured from the cells growing in 96-well plate using XTT assay kit (Cell Signaling Technology) following manufacturer’s instruction using BioTek Synergy microplate reader.
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bioRxiv - Developmental Biology 2024Quote: ... The cells were collected according to the manufacturer’s instructions for the Cut and Run assay kit (Cell Signaling, 86652). 10 μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: Cleaveage Under Targets and Release Using Nuclease experiment was performed using a CUT & RUN Assay Kit (Cell Signaling, USA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Supershift assays were performed by incubating monoclonal antibody (Ab) to specific AP-1 transcription factors c-Jun (60a8, Cell Signaling), c-Fos (9F6) ...
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bioRxiv - Cell Biology 2022Quote: ... adipocytes were treated with assay medium +/-insulin for 10 minutes and harvested in cell lysis buffer (Cell Signaling Technology #9803) with phosphatase inhibitor (Thermo Fisher Scientific 78442) ...
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bioRxiv - Bioengineering 2020Quote: ... BrdU was added to the cells and proliferation was assessed 12h later using a colorimetric BrdU assay (Cell Signaling, Inc.).
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bioRxiv - Microbiology 2021Quote: Kinase assay was performed using recombinant proteins purified from E.coli and commercially available 10X Kinase buffer (Cell Signaling Technology, #9802). Recombinant proteins (N’-His PINK1 and N’-His Ajuba ...
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bioRxiv - Microbiology 2021Quote: ... The antibodies used in the WB assay included anti-PDGFRβ rabbit mAb (1:1000, Cell Signaling Technology, Danvers, CA, USA), anti-JEV NS3 rabbit antiserum (kindly provided by C.J ...
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bioRxiv - Immunology 2020Quote: Cells were collected and lysed on ice for 30 min in 1X radioimmunoprecipitation assay (RIPA) buffer plus (Cell Signaling Technology) with protease and phosphatase inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Intracellular cAMP concentrations were determined using a cyclic AMP XP Assay Kit (Cell Signaling Technology, catalog number 4339S, Danvers, MA) using 50μg total protein per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: S2R+ cells expressing Flag-dwg were subjected to ChIP assays using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... cGMP was measured in duplicate according to manufacturer’s directions using the Cyclic GMP XP® Assay Kit (Cell Signaling #4360) after the supernatant was diluted 1:10 in 1× Cell Lysis Buffer (Cell Signaling #9803) ...
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bioRxiv - Microbiology 2023Quote: Cell lysis was performed using Radioimmunoprecipitation (RIP) assay buffer (#9806, Cell Signaling Technology, 3 Trask Lane, Danvers, MA 01923, USA) supplemented with protease and phosphatase inhibitor (#5872) ...
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Targeting adipocyte ESRRA promotes osteogenesis and vascular formation in adipocyte-rich bone marrowbioRxiv - Molecular Biology 2023Quote: ... These cells were then subjected to ChIP assays with primary antibody against ESRRA (10 μl/IP, Cell Signaling Technology #13826) or normal rabbit IgG ...
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bioRxiv - Developmental Biology 2024Quote: The following antibodies were used in the immunostaining assays: Alexa Fluor® 488 Phalloidin (Cat# 8878, Cell Signaling, 1:200), anti-Myosin (Cat# A4.1025 ...
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bioRxiv - Cell Biology 2020Quote: ... The pull-down assay of ZFP982-MYC tagged protein was performed through co-Immunoprecipitation (co-IP) using MYC-Tag (9B11) antibody (#2276, Cell Signaling). For MYC-co-IP ...
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bioRxiv - Immunology 2021Quote: ... anti-β-actin (3700) and the secondary antibodies used for western blot and immunofluorescence assay were purchased from Cell Signaling Technology. Anti-SYVN1 (ab170901 ...
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bioRxiv - Neuroscience 2021Quote: Pericytes viability was assessed using the 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay according to the manufacturer’s procedure (Cell Signaling Technology). Cells were plated at the appropriate concentration in 96-well plates under different experimental conditions ...
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bioRxiv - Cell Biology 2021Quote: Chromatin immunoprecipitation assay was performed using SimpleChIP® Enzymatic Chromatin IP Kit - Magnetic Beads (Cell Signaling Technology, Inc., Danvers, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: CUT&RUN experiments were conducted using 200,000 PS or PSP cells using the CUT&RUN assay kit (Cell Signaling Technology, 86652). Briefly ...