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Citations for Cell Signaling Technology :
2201 - 2250 of 2267 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Samples were transferred to a PVDF membrane and blocked for one hour in 5% dry milk before incubation with KIF3A (D7G3) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, #8507) in TBS containing 0.1% Tween-20 and 5% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody dilutions were prepared in 5% BSA-TBST and incubated overnight with gentle agitation at the following dilutions phospho-AKT T308 1:2000 (Cell Signaling Technology Cat# 13038, RRID:AB_2629447), AKT 1:1000 (Cell Signaling Technology Cat# 9272 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was washed with TBST and incubated in TBST + 5% (w/v) nonfat milk and anti-rabbit IgG HRP-linked antibody (Cell Signaling catalog #7074, 1:1000) for 1 hour then washed in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... we developed our RiboAb cocktail (20 µL used at a dilution of 1:1000) containing 5 µL rpl5 (Cell Signaling, Danvers, MA, USA, cat #: 14568S), 5 µL rpl11 (Cell Signaling ...
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bioRxiv - Genetics 2024Quote: ... Membranes were blocked in 5 % milk for 1 hour followed by overnight incubation with PTEN primary antibody (Cell Signaling Technology, cat# 9188; 1:1000 dilution) or GAPDH primary antibody (Santa Cruiz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were washed four times for 5 min each and treated with 1:1000 diluted Alexa Fluor® conjugated sec antibody (Cell Signaling Technology, Danvers, MA, USA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:100), Rat anti-GFP monoclonal antibody (nacalai tesque, GF090R, diluted 1:1000) and rabbit anti-pSmad1/5/9 antibody (Cell Signaling Technology, #13820, diluted 1:100). After the primary antibody incubation ...
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bioRxiv - Molecular Biology 2023Quote: The membranes were blocked with 5% BSA at room temperature for 30 min and incubated with anti-eNOS (clone: D9A5L, Cell Signaling Technology, MA, USA, 1:1,000) and anti-Ser177 p-eNOS (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked in 5% milk and probed with primary antibodies (HA-BioLegend Clone HA.11, H3-Abcam, CDH2-Sigma-Aldrich, TWIST1-Santa Cruz, Vimentin-Cell Signaling Technology, GAPDH-GeneTex) overnight ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C overnight with primary antibodies (HIF1α, 1:1000, Novus Biologicals, NB100-134; HIF2α, 1:1000, Cell Signaling Technology, 7096; β-Actin, 1:5000, Merck, A1978) in 0.1% skimmed milk in TBST buffer (blocking buffer) ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Microbiology 2020Quote: ... blocked with 5% skimmed milk powder at room temperature for 1 h, the membrane was incubated with the target primary antibody with desired dilution (anti-ACE-2, 1:1000 (Cell signaling technology, MA, USA); TMPRSS2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-phospho-SAPK/JNK antibody (#9251) and anti-phospho-p44/42 MAPK (Erk1/2) antibody (#9101) were obtained from Cell Signaling Technology (MA, USA). The PPARγ inhibitor ...
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bioRxiv - Biochemistry 2021Quote: Cleaved-caspase-3 immunostaining was performed on 2 µm thick formalin-fixed paraffin sections in a 1:200 dilution according to the manufacture’s protocol (Cell Signaling Technology, Danvers, MA, USA). Pictures were taken on a slide scanner SCN4000 in a 20× magnification.
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Cancer Biology 2021Quote: ChIP was performed on 5X106 cells from 2-day control and radiated gliomaspheres using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, #9005S) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... hypoxia inducible factor 1 subunit alpha (HIF1A; 1:1000, no. #14179), Bax (1:1000, no. #2772), and Bcl-2 (1:1000, no. #4223) (Cell Signaling Technology, Inc., USA). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... pEGFR (Y1068, #3777) pAKT (S 473, #9271 and p44/42 MAPK (ERK1/2) (T202/Y204, #4370) were purchased from Cell Signaling Technology (Danvers, MA), β- actin was used as a housekeeping proteinand the antibody was bought from EMD Millipore (#MAB1501 now part of Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The NC membrane was incubated for 1 hour at RT in blocking buffer (5% nonfat milk in PBS [pH 7.4] containing 0.1% Triton X-100 and 0.02% NaN3) and immunoblotted for 2 hours at RT with antibodies to CFTR (1:1000, Cell signaling Technology Cat. No. 78335,), SNAP-23 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...
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bioRxiv - Cell Biology 2023Quote: ... 10% FBS) and incubated with the following primary antibodies in blocking buffer for 2 hours at RT: YAP (Cell Signaling Technology, #14074, 1:100), TAZ (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... and MAPK activation was assessed by immunoblot analysis using primary anti-Phospho-p44/42 MAPK antibody (Erk1/I 2; Thr-202/Tyr-204) (Cell Signaling, Danvers, MA, USA) and secondary goat anti-rabbit-HRP antibody (Cell Signaling ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was blocked with 2%BSA overnight and was probed with antibodies against goat anti-FLAG (1:1000, Cell Signaling Technologies, D6W5B, #14793), alpha Tubulin (1:10000 ...
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bioRxiv - Developmental Biology 2024Quote: ... antibodies specific to phosphorylation of serine 473 of Akt1/2/3 were used to detect pAkt (1:300, 4060S, Cell Signaling Technology, Danvers, MA); and antibodies specific to phosphorylation of tyrosine 783 of PLCγ1 were used to label pPLCγ (1:250 ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... which were diluted to 1:100 in 5% BSA and 0.5% Triton X-100 in PBS: anti-BECN1 (#3738; Cell Signaling Technology and sc-48341; Santa Cruz Biotechnology), anti-PLIN1 (#9349 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked in 0.1% Tween PBS supplemented with 5% (w/v) skimmed milk and subjected to immunoblotting with the following primary antibodies at the indicated dilutions: TBK1 (Cell Signaling [CS-3013], rabbit polyclonal, 1:500); GAPDH (Invitrogen [AM4300] ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology, A21247 Life Tech). F-actin was stained with Alexa Fluor-conjugated Phalloidin (1:40 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 30min in the primary + secondary antibody solution: 2%BSA-PBS + Rabbit anti-mouse Phosopho-Akt (Ser473) antibody (Cell Signaling Technologies 4060; 1:50), Goat anti-Rabbit Alexa 488 secondary antibody (ThermoFisher A32731 ...