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Citations for Cell Signaling Technology :
1851 - 1900 of 2267 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... fresh frozen OCT-embedded embryo lung sections (5 μm thick) were stained with anti-E-Cadherin (Cell Signaling, Cat No 3195), anti-Snail (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... The protein A agarose bead was added with 450 µl of IP buffer and 5 µl of Myc antibody (Cell Signaling). The mixture was then incubated on rotator at 4°C for 4 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Membranes were then incubated o/n at 4C with 5% milk powder in PBS-T containing the primary antibodies at the following dilutions (1:1000 Lin28a (A177, Cell signaling); 1:4000 H3 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: The following antibodies (in 5% milk in TBST) were used for western blotting: anti-E-cadherin (#14472, monoclonal mouse, 1:1000, Cell Signaling), anti-N-cadherin (#BDB610920 ...
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bioRxiv - Immunology 2021Quote: ... Cells were treated with 5% goat serum for 30 min and incubated with antibodies against TRPC3 (Alomone Labs) or NFkB-p65 (Cell Signaling) for 1h ...
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bioRxiv - Developmental Biology 2022Quote: ... The soft palatal tissue from E13.5 embryos was collected and fixed according to the manufacturer’s instructions based on Cut and Run assay kit (Cell Signaling, 86652). Ten μl of IgG isotype control (Cell Signaling ...
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bioRxiv - Cancer Biology 2022Quote: ... The chromatin was then incubated with 5 µg of antibody against KDM6A (A302-374A, Bethyl Laboratories and 33510S, Cell Signaling Technology), KDM6B (ab169197 ...
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bioRxiv - Cancer Biology 2022Quote: β-Galactosidase staining at pH 6.0 was performed with 5 × 105 cells using the Senescence β-Galactosidase Staining kit (Cell Signaling # C10841) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... followed by blocking in 5% BSA with 0.2% Triton X-100 for 30min and incubation with primary antibody Axin1 (#2087, 1:200, Cell Signaling Technology) overnight ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with PBS-0.1% Tween 20 (PBS-T) containing 5% milk and were incubated with rabbit monoclonal anti-CYLD (1:500, #8462 Cell Signaling) and mouse monoclonal anti-β-actin (1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and the grey matter was Dounce homogenized in 5 volumes per weight (v/w) buffer containing phosphate buffer saline (PBS) and 1X protease inhibitor (#5871, Cell signaling) in a 15 ml glass homogenizer with 30 up and down strokes at 70% power by hand on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was then incubated with 5 μg of Anti-FLAG Antibody (DYKDDDDK Tag (D6W5B) Rabbit mAb#14793 antibody (Cell Signaling) and 50 μL of protein G beads at 4 ºC overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were washed 3x 5 mins with PBS and then stained with corresponding secondary antibodies (1:1000; 4414S Cell Signaling Technology, 8889S Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking solution was replaced with 1° antibody (Ab) (Phospho-Smad1/5/9 Rabbit mAb #13820, Cell Signaling Technology 13820T, 1:300) in 10%NGS/PBS-Tx and the slides were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... All antibodies were diluted in 1X PBS with 0.3% Triton X-100 and 5% bovine serum albumin (BSA, Cell Signaling Technology).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked for 1h and probed with 5% nonfat dry milk with the following primary antibodies: anti-IRF8 (clone D20D8, Cat# 5628, Cell Signaling), anti-CD74 (sheep anti-mouse cd74 ...
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bioRxiv - Cancer Biology 2023Quote: ... All antibodies were diluted 1:1000 in 5% milk/TBST (FGFR1: D8E4; GAPDH HRP-conjugated: 14C10; Cell Signaling, Danvers, MA, USA). The membranes were incubated with antibodies at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized with 100% cold methanol and stained with anti-phosphorylated (p)SMAD1/5/9 (Cell Signaling Technology, Danvers MA) overnight at a 1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... were blocked in TBST buffer (Tris-buffered saline and 0.15% Tween-20) containing 5% nonfat dry milk (#9999, Cell Signaling Technology). The blots were probed overnight with primary antibodies diluted in blocking buffer ...
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bioRxiv - Immunology 2024Quote: ... Membranes were blocked for 1 h in 5% non-fat milk in 1X TBST at room temperature and incubated with primary antibodies against KEAP1 (#8047, Cell Signaling), NQO1 (#11451-1-AP ...
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bioRxiv - Physiology 2024Quote: ... C2C12’s were replated and allowed 24 hours to recover after which the labeled C2C12 myoblasts were induced for apoptosis using 5 µM Staurosporin (cat. no.: 9953S, Cell Signaling) for 18h ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then blocked and permeabilized for 1 hour at room temperature while rocking with 1mL of blocking buffer (0.3% Triton X 100 and 5% normal goat serum (Cell Signaling Technology) in 1x PBS ...
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bioRxiv - Physiology 2024Quote: ... Triton X-100 CD31+ and CD31-cell lysates (5-15 µg/lane) were analyzed by western blot for proteins and eNOS (#32027; 1:500; Cell Signaling) and GAPDH (#97166 ...
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bioRxiv - Neuroscience 2024Quote: ... washed in TBST-Tween20 and blocked for 1h at room temperature with TBST/5% Normal Goat Serum (Cell Signaling Cat# 5425). Following blocking ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were washed three times with PBS-T for 5 minutes each.The wells were then incubated with Mouse Anti-Myc antibody (9B11, Cell Signaling Technology) diluted 1:3000 in PBS-T with 5% BSA for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cells were incubated with primary antibody anti-phospho-histone H2A.X (γH2AX) (#2577; Cell Signaling. 1:200 in 5% BSA in PBS), for 1 hour ...
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bioRxiv - Biophysics 2024Quote: ... Knockdown efficiency was validated by immunoblotting with pannexin-1 primary antibody (Cell Signaling Technologies, 91137, 1:1000 in 5% BSA-TBST).
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bioRxiv - Cancer Biology 2024Quote: ... followed by 3 5-minute washes by 1x TBST and probing with horseradish peroxidase conjugated secondary antibodies (Cell Signaling, 7074, 7076) for 1-1.5 hours at 25°C ...
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bioRxiv - Cancer Biology 2024Quote: ... then stained overnight with primary antibodies in blocking buffer (5% goat serum or SignalStain antibody diluent, Cell Signaling Technology, cat#8112). For non-CST primary antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... or Phospho-p44/42 MAPK (erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit monoclonal antibody (Cell Signaling Technology; 4370). Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat (A0545 ...
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Wnt-dependent activation of ERK mediates repression of chondrocyte fate during calvarial developmentbioRxiv - Developmental Biology 2021Quote: ... Western Blots were performed with the following primary antibodies: rabbit-anti-p-ERK1/2 (1:500, Cell Signaling 9101), rabbit-anti-Erk1/2 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 μg of nuclear protein lysates were incubated with 2 μg of either Myc-Tag antibody (Cell Signaling Technology) or an endogenous NUP210-specific antibody (Bethyl Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody used for immunofluorescence include anti-phospho-p44/p42 ERK1/2 (Thr202/Tyr204) (Cat# 4370) from Cell signaling technologies used at 1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... and Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (clone D13.14.4E) (#4370; 1:1000) were purchased from Cell signaling. M2 anti Flag Mouse antibody (#SLBT7654 ...
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bioRxiv - Cell Biology 2022Quote: ... and the supernatant containing fragmented chromatin was incubated with 2 μl of anti-YY1 antibody (D5D92Z, Cell Signaling, 46395) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 10 μM of p38 inhibitor (SB203580) or MEK1/2 inhibitor (U0126) was used (Cell Signaling Technology, Danvers, MA, USA). Cells were monitored by live cell imaging using the BD Pathway 855 Bioimaging System (BD Biosciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Ward) at 1:400 concentration or a rabbit anti-phospho-Myosin Light Chain 2 (Ser19) (#3671, Cell Signaling Technology) at 1:50 concentration ...
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bioRxiv - Developmental Biology 2020Quote: Cell lysates were prepared in 1x SDS Sample Buffer (62.5 mM Tris-HCl pH 6.8, 2%SDS, 42mM DTT, 10% glycerol and 0.01% bromophenol blue; Cell Signaling Technology), sonicated to shear DNA and reduce viscosity and then heat denatured ...
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bioRxiv - Developmental Biology 2022Quote: ... the following primary antibodies were used: Rabbit monoclonal antibody (mAb) to Snail-2 (clone C19G7, Cell Signaling, 1/300), mouse mAb to Sox-10 (Clone A2 ...
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bioRxiv - Cancer Biology 2022Quote: ... antibodies (RRID:AB_330744) and monoclonal mouse anti-phospho-p44/42 MAPK antibodies (pERK1/2; pT202/pY204) (RRID:AB_331768) were from Cell Signaling.
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bioRxiv - Molecular Biology 2024Quote: ... and the anti-phospho-Thr202/Tyr204-ERK1/2 polyclonal antibody were purchased from Cell Signaling Technologies (Beverly, MA, USA). The anti-cyclin B1 monoclonal antibody was from BD Biosciences Pharmingen ...
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bioRxiv - Immunology 2024Quote: ... The fixed cells were incubated for 2 hrs at room temperature with β-actin antibody (Cell Signaling Technology, 4967) at 1:1000 dilution and then incubated with VRDye 549 Goat anti-Rabbit IgG Secondary Antibody (LI-COR biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... blocked in 2% BSA for 1h and stained with primary anti-UBA1a/b antibodies (Cell Signaling, 4891S, 1:500) for 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 μg of protein were added to pre-washed 50 μL streptavidin Sepharose bead conjugates (Cell Signaling Technology, 3419S) and rocked overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (4370, 1:1000, Cell Signaling Technology), anti-CD9 (ab307085 ...
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bioRxiv - Developmental Biology 2024Quote: ... the following primary antibodies were used: Rabbit monoclonal antibody (mAb) to Snail-2 (clone C19G7, Cell Signaling, 1/300), mouse mAb to Sox-10 (Clone A2 ...
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bioRxiv - Biochemistry 2024Quote: ... and negative control IgG from the same species (Cell Signaling Technology, 2729; 2 µg antibody for 0.5 mg lysate). For detection of protein-antibody complex ...
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bioRxiv - Neuroscience 2021Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...