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Citations for Cell Signaling Technology :
2151 - 2200 of 2211 citations for 8 3 5 DIMETHYL 4 METHOXYPHENYL 8 OXOOCTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was transferred to new tubes and antibody was added and allowed to bind overnight at 4 °C with rotation (15uL antibody per tube, Cell Signaling Technology D31H2 anti-CTCF, 3418S). 30uL of pre-washed Protein G Dynabeads were added to each tube and rotated at 4 °C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... The following primary antibodies were added after blocking for an hour with 5% non-fat milk: STAT3 and Tyr705 pSTAT3 (Cell Signaling Technology, MA, USA), GAPDH (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% no-fat dry milk in TBST and immunoblotted with rabbit polyclonal anti-CHD7 1:2000 (Cell Signaling Cat. No.6505S), rabbit polyclonal anti-SOX2 1:2000 (Millipore Cat ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used in ChIP are: rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 5 µL per IP); mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Immunology 2021Quote: Cells were fixed with 0.25% paraformaldehyde in ice-cold PBS for 5 min and incubated with anti-ERp57 (#A484, Rabbit mAb, Cell Signaling Technology, Danvers, MA, USA) 1:100 for 40 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Cells were then resuspended in 100 μl PBS-FC containing 5% FBS with 1:100 diluted PE-conjugated anti-myc (9B11) mouse monoclonal antibody (Cell Signaling Technology, Danvers, MA) and incubated for 30 min in the dark ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked in 5% BSA in TBS-T before incubation with primary antibodies: phospho-AMPKα Thr172 (1:1000, Cell signaling, MA, USA #2535), β-actin (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling, pTYR 4G10 Millipore) and secondary 1h antibodies coupled to HRP ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was blocked for 2-hours in 1% NGS and 0.3% Triton X-100 followed by 2-3 days of 4C° incubation in 1xPBS (5% NGS, 0.3% Triton X-100) with primary antibody(ies): pS6 S235-236 (Cell Signaling, Catalog: 4858, 1:500), pS6 S244-247(ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS and incubated with anti-APRT antibody or an anti-β- Actin antibody (Cell Signaling Technology, USA) at 4°C overnight followed by the corresponding secondary antibody for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 5% BSA in TBS-T and probed with primary antibodies recognizing β-Actin (Cell Signaling Technology, #3700, 1:4000, Danvers, MA, USA), c-Myc (Cell Signaling Technology ...
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FGF signalling is involved in cumulus migration in the common house spider Parasteatoda tepidariorumbioRxiv - Developmental Biology 2021Quote: ... Fluorescent pMad antibody staining was performed using the Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb (Cell Signaling Technology, Inc.; antibody concentration: 1:1000) as primary and an Alexa488 anti rabbit AB (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed three times for 5 min per time with shaking then incubated with secondary antibodies (anti-mouse IgG, HRP-linked antibody, Cell Signaling, Danvers, MA, USA) diluted in 5% w/v nonfat milk at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated overnight at 4°C with antibodies for the designated antigens INTEGRIN α5 (1:1000, #4705), INTEGRIN β3 (1:1000, #4702), β-ACTIN(1:1000, #8457), β-TUBULIN (1:1000, #2146) purchased from Cell Signaling Technology, GAPDH (1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution, or rabbit anti-pMET, #3077, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Physiology 2023Quote: ... blots were washed with TBS-T three times and then incubated in TBS-T containing 5% non-fat dry milk and anti-biotin horseradish peroxidase (Cell Signaling, Danvers, MA, USA) and HRP-conjugated donkey anti-chicken antibody (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... and then incubated with the following primary antibodies for 2 hours at room temperature in 5% BSA: 1:1000 anti-SMARCC1 (Cell Signaling Technologies cat. #11956T), 1:1000 anti-SMARCA4 (Cell Signaling Technologies cat ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked in Tris-buffered saline/0.1% Tween (TBST)/5% milk and probed with antibodies against E-Cadherin (Cell Signaling Technology, 3195S, 1:1000 dilution), Vimentin (Cell Signaling Technology ...
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: 5 μM thick paraffin-embedded liver sections were used for hematoxylin and eosin (H&E) staining and Ki67 (Cell signaling technology 12202S, 1:400) immunohistochemical staining ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was blocked with milk-TBST (5% skimmed milk, 1xTBS and 0.1 % Tween-20) and then incubated with anti-STAT1 (Cell Signaling Technology Cat# 9172, RRID:AB_2198300), anti-pSTAT1 (Cell Signaling Technology Cat# 9167 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies, Cat. # 13174) and an anti- rabbit HRP secondary antibody (Cell Signaling Technologies ...
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bioRxiv - Genetics 2019Quote: ... the membranes were incubated for 12 hours or longer at 4°C with rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 1:1000 dilution) or mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
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bioRxiv - Molecular Biology 2019Quote: ... 20 µL of samples from each fraction were lysed with 5 µL 1:1 mixture of RIPA buffer (Cell Signaling Technology, Danvers, MA. Cat #: 9806S) and protease inhibitor (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then washed three times for 5 minutes each with 1X TBST and incubated with a secondary anti-rabbit horseradish peroxidase-conjugated antibody (Cell Signaling, Cat # 7074S, 1:2000) in 5% BSA for two hours at room temperature with gentle rocking ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were then blocked with 5% nonfat milk at room temperature for 1 hour and then probed with primary antibodies (phospho-p44/42 MAPK, Cell Signaling 9101, 1:4000, dilution) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... The membrane was washed again 4 times in TBST for 5 m each and blocked again before incubating with primary antibodies: total Akt (1:1000, Cell Signaling Technology, Cat. #9272S, RRID:AB_329827) & total S6RP (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.0% Tween-20) for 1 hour at room temperature and probed with primary antibody (1:1000 HA-Tag Rabbit mAb, Cell Signaling, #3724, in 5% milk) overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were transferred to a PVDF membrane and blocked for one hour in 5% dry milk before incubation with KIF3A (D7G3) rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, #8507) in TBS containing 0.1% Tween-20 and 5% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2019Quote: Immunohistochemical stainings of pERK1/2 and pS6 were performed on 5 μm thick coronal sections of paraffin-embedded mouse brains using primary antibodies raised against pERK1/2 (1:300, Cell Signaling Technology Cat# 4370, RRID:AB_2315112) and pS6 (1:300 ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133; V5 tag, ThermoFisher #R960-25; ETV6, Santa Cruz, # sc-166835x; ERG, Cell Signaling Technologies # 97249). Subsequent chromatin capture ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies were incubated for 2 h in a solution of TBST (0.05%) and LI-COR blocking buffer (5%) containing the following antibodies: anti-ERK1/2 (Cell Signaling Technologies; dilution factor: 1/2000) and streptavidin IRdye680 (LI-COR Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody dilutions were prepared in 5% BSA-TBST and incubated overnight with gentle agitation at the following dilutions phospho-AKT T308 1:2000 (Cell Signaling Technology Cat# 13038, RRID:AB_2629447), AKT 1:1000 (Cell Signaling Technology Cat# 9272 ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...