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Citations for Cell Signaling Technology :
1901 - 1950 of 2211 citations for 8 3 5 DIMETHYL 4 METHOXYPHENYL 8 OXOOCTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in 5% milk before probing with the following antibodies: rabbit anti-SHIP1 (1:1000, Cell Signaling D1163) and rabbit anti-β actin (1:2500 ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were then blocked with 5% non-fat dry milk in Tris-buffered saline (#9997, Cell Signaling Technology, USA) with 0.1% Tween 20 (P1379 ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunoprecipitations were carried out as previously described63 on approximately 5 ug of chromatin (minimum 2.5 ug, maximum 8.8 ug) using 5 ul of total RNA Pol II antibody (Cell Signaling D8L4Y). Libraries were prepared using the NEBNext Ultra II DNA Library Kit for Illumina (NEB ...
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bioRxiv - Biophysics 2023Quote: ... blots were incubated in blocking buffer (5%(w/v) milk powder (nonfat dry milk, Cell Signaling Technology, Danvers, MA, USA)) in TBST containing 25 mM Tris base ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA TBST for 1h at 24 prior to incubating with STAT3 (Cat #12640, RRID:AB_2629499, Cell Signaling Technologies) or NF- kB (p65 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nitrocellulose membrane was blotted with primary antibodies in TBS containing 0.05% Tween and 5% BSA (Cell signaling; p65-RelA (8242), phospho-p65-RelA-Ser536 (3033) ...
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bioRxiv - Cell Biology 2023Quote: ... and staining was performed after blocking with 5% normal goat serum with anti-Insulin (Linco) and anti-Glucagon (Cell Signaling) antibodies using established protocols ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was blocked in 5% milk and incubated with primary antibody: anti-Phospho-IRF3 (Ser 396) (D601M, Cell Signaling), anti-IRF3 (D6I4C ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was blocked with 5% milk in 1X TTBS and incubated overnight with the appropriate primary antibody (Cell Signaling c-IAP2 58C7 Rabbit mAb #3130 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were washed 3X 5 min with TBS-T and incubated with secondary antibody at 1:500 (#7074, Cell Signaling) in 1% BSA in TBS-T for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... incubated in primary antibody 1:20 mouse anti-AP2 (Developmental Studies Hybridoma Bank, University of Iowa, 3B5 supernatant) and 1:300 rabbit anti-Cleaved Caspase 3 (Cell Signaling Technology, Danvers, MA #9661) o/n in humid chamber ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168, 1:1000 anti-PARP Cell Signaling Technologies #9542 ...
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bioRxiv - Neuroscience 2024Quote: ... Representative 20X maximum intensity projections of apoptotic cells in the ganglion cell layer labeled by primary antibody against cleaved (active) caspase-3 (Cell Signaling Technology Cat# 9661, RRID:AB_2341188).
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% milk in TBS/0.1% Tween-20 (TBST) and incubated with anti-phospho-eNOS (Thr495) and anti-eNOS (Suppl Table 3; 1:1,000, Cell Signaling cat. #9574 and 9572, respectively). Membranes were washed in TBST ...
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bioRxiv - Developmental Biology 2023Quote: We used an antibody for phospho histone 3 (pH3) that was previously reported(Shirae-Kurabayashi et al, 2006) (pH3-ser10-6g3-mouse-mAb, Cell Signaling; #9706; 1:500 diluted). The primary antibody was added together with anti-DIG antibody during FISH process ...
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bioRxiv - Cancer Biology 2024Quote: Quantification of hepatocyte and tumor cell apoptosis was performed in liver slides by anti-cleaved caspase-3 immunostaining (Cell Signaling Technology #9661, diluted 1:400), detected with HRP-bound goat anti-rabbit secondary antibody (Invitrogen #31460 ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were incubated overnight at 4 °C with the primary antibodies NF-κB p65 Mouse mAb (Cell Signaling Technology, diluted 1:1000) or TBP Rabbit mAb (Cell Signaling Technology ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Neuroscience 2020Quote: ... were lysed in complete RIP lysis buffer and the cell lysates were divided into equal parts and incubated (overnight with rotation, at 4°C) with anti-Pol II (Cell Signaling Technology (CST), Cat ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then fixed with 4% paraformaldehyde and processed for immunostaining with EGFR (D38B1) XP rabbit mAb (4267, Cell Signaling Technology, 1:100), anti-rabbit secondary antibody Alexa Fluor 555 Conjugate (4413 ...
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bioRxiv - Cell Biology 2020Quote: SMC cultured on glass coverslips were fixed in 4% PFA prior to immunostaining with phospho-histone H2AX (γH2AX) antibody (Cell Signaling Technologies #2577) as described previously (19) ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
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bioRxiv - Systems Biology 2022Quote: ... blocked in 5% dry milk (American bioInc) for 60 min followed by incubation overnight (at 4°C) with primary antibody according to manufacturer’s instruction (Cell Signaling Technology, pSMAD3 (9520S) SMAD3 (9513S) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked followed by overnight incubation at 4°C with primary antibodies: C-terminal Parkin antibody (PRK8; Cell Signaling, 1:40,000 dilution) and N-terminal Parkin antibody (https://mrcppureagents.dundee.ac.uk/reagents-view-antibodies/589302 ...
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bioRxiv - Cell Biology 2019Quote: ... Caco-2 cells were incubated overnight at 4°C with primary antibodies (1:100 dilutions with blocking buffer) for GRP78 (Cell Signaling, MA, USA) or CHOP (Santa Cruz ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Pathology 2021Quote: ... and incubated with primary antibodies at 4°C overnight at the following concentrations: β-catenin (Cell Signaling, 8480, 1:1000 in 1% BSA), p65 (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with hematoxylin-eosin staining for 10min or overnight at 4℃ using the following primary antibodies: β-catenin (1:200, #8480, Cell Signaling Technology), Ki67 (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with ice-cold 100% (v/v) methanol for 4□min and then incubated with anti-LC3B (3868; Cell Signaling Technology, MA), anti-LAMP-2A (ab18528 ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 200,000 Bead-bound cells were incubated with primary antibody overnight at 4°C (1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173), 1:50 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... All cell pellets were then resuspended in 100 µL fresh MG medium and stained with 1.4 µM 4’,6-diamidino-2-phenylindole (DAPI) from Cell Signaling Technology (Cat #4083S) for 15 min in the dark at 30 °C in the microaerobic chamber ...