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Citations for Cell Signaling Technology :
1951 - 2000 of 10000+ citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1:1000 IB), Lck (#2984, 1:1000 IB), LckY505 (#2751, 1:1000 IB), and SrcY416 (#6943, 1:2000 IB) from Cell Signaling Technology; ZAP-70 (X17371 ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-phospho-TBK1 (D52C2, dilution 1:1000), rabbit anti-IRF3 (D83B9, dilution 1:1000), rabbit anti-phospho-IRF3 (4D4G, dilution 1:1000) (Cell Signaling Technology); rabbit anti-TBK1 (ab40676 ...
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bioRxiv - Biophysics 2022Quote: ... 0.1% Tween-20) for 1 hour and blotted with primary antibody (anti-TCOF1, Proteintech, 11003-1-AP; anti-HA, Cell Signaling (C29F4), 3724S ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100uM glycine for 30 minutes at room temperature and labeled overnight at 4C with primary antibodies (rabbit anti-FOXP2 Abcam #16046 1:500, rat anti-Flag 1:500 Novus NBP1-06712, mouse anti-NeuN Ab 1:500, Cell Signaling #94403S). For secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1, 1:1000, Cell Signaling; rabbit anti-AKT2, 1:1000, Cell Signaling; rabbit anti-AKT3 ...
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bioRxiv - Cancer Biology 2023Quote: ... TritonX-100 in PBS for 1 h at room temperature and then incubated with primary antibody p-STAT-1 (Tyr701) (1:500; Cell Signaling Technology), or CD3χ (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies (γH2AX 1:300, Cell Signaling Technology, Cat#2577; RPA 1:300, Millipore, Cat#MABE285; FOXM1pT600 1:1000, Cell Signaling Technology, Cat#14655 ...
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bioRxiv - Immunology 2023Quote: ... blocked with TBST (1x Tris buffered saline with 0.1% Tween 20) supplemented with 5% (wt/vol) BSA for 1 hr and blotted with PU.1 (9G7) (1:1000, Cell Signaling #2258) at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:1000, Sigma; anti-DCTPP1 antibody, 1:500, Abcam; anti-XPB antibody, 1:500, Abcam; anti-β-actin antibody, 1:5000 Cell Signaling) overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were then incubated overnight at 4°C with primary antibody (GAPDH, 1:50,000 dilution, Cell Signaling #2118; α-MTR, 1:1,000 dilution, ProteinTech #25896; DHFR, 1:500 dilution, Cell Signaling #45710) in 5% bovine serum albumin (BSA ...
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bioRxiv - Genomics 2021Quote: ... Nuclear stain solution was prepared for 4 slides at a time: 20 µLSYTO13 (Nanostring) and 180 µL 1x TBS (Cell Signaling Technologies, 12498S) for a total volume of 200 µL/slide ...
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bioRxiv - Neuroscience 2020Quote: ... were lysed in complete RIP lysis buffer and the cell lysates were divided into equal parts and incubated (overnight with rotation, at 4°C) with anti-Pol II (Cell Signaling Technology (CST), Cat ...
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bioRxiv - Cell Biology 2020Quote: SMC cultured on glass coverslips were fixed in 4% PFA prior to immunostaining with phospho-histone H2AX (γH2AX) antibody (Cell Signaling Technologies #2577) as described previously (19) ...
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bioRxiv - Molecular Biology 2022Quote: ... The blocked membrane was incubated overnight at 4°C with primary antibodies diluted in 1x Tris buffered saline with Tween-20 (TBST, Cell Signaling Technology: 9997S). The membrane was washed twice using TBST ...
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bioRxiv - Systems Biology 2022Quote: ... blocked in 5% dry milk (American bioInc) for 60 min followed by incubation overnight (at 4°C) with primary antibody according to manufacturer’s instruction (Cell Signaling Technology, pSMAD3 (9520S) SMAD3 (9513S) ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was blotted with 5% nonfat milk in TBST at RT for 1h and then incubated with the following antibodies at 4°C overnight: TWIST1(Cell Signaling Technology, 46702S), E-cadherin (Cell Signaling Technology ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% paraformaldehyde for 5 min and subsequently permeabilized with 0.2% Triton-X and stained with anti-relB (Cell Signaling Technology, Danvers, USA) followed by goat-anti-rabbit Alexa 546 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with ice-cold 100% (v/v) methanol for 4□min and then incubated with anti-LC3B (3868; Cell Signaling Technology, MA), anti-LAMP-2A (ab18528 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C with the primary antibody diluted in blocking buffer or SignalStain® Antibody Diluent (cat. 8112, Cell Signaling Technology). Slides were incubated with biotinylated secondary antibodies for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatants were incubated in rotation at 4°C for 20 hours with a protein complex of anti-HA antibody (Cell signaling technology, C29F4) and Sera-Mag SpeedBeads Protein A/G (Cytiva) ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Physiology 2024Quote: ... Protein content was measured using DC Assay and samples processed as above to probe for activating transcription 4 (ATF4) abundance (Cell Signaling cat. 11815) as a marker of ER stress.
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bioRxiv - Microbiology 2024Quote: ... The membranes were blocked and then incubated overnight at 4°C with the primary antibodies: Tubulin (Sigma, Cat.#T6074; Cell signaling, Cat.#2144), HA-tag (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were incubated with primary antibodies overnight at 4°C and then probed with secondary antibodies: horse anti-mouse IgG (Cell Signaling Technology #7076) or goat anti-rabbit IgG (Cell Signaling Technology #7074) ...
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bioRxiv - Cell Biology 2024Quote: ... cardiac myocytes were incubated with primary antibodies in blocking buffer at 4°C overnight as follows: P-S6 Ribosomal ProteinS235/2361:500 (cat# 2211, Cell signaling, Danvers, MA); RyR2 1:500 (cat# MA3-916 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following primary antibodies were diluted 1:1000 in 3% BSA/T-TBS and incubated for about 16 h: anti LC3-I/II (cat. # 12741S) from Cell Signaling Technologies (distributed by Euro-Clone); anti-α-Tubulin (cat ...
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bioRxiv - Cell Biology 2020Quote: ... WB dilution 1:1,000) and P-MLC2 (T18/S19, #3674S IF dilution 1:100, WB dilution 1:500) were from Cell Signaling (Danvers, MA, USA). Rabbit monoclonal antibody directed against α4-actinin was from Life Technologies (#42-1400 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated overnight with primary antibodies (1:500 pSTAT1, 7649 Cell Signaling; 1:500 pSTAT3, 9145 Cell Signaling; 1:500 pSTAT5, 9359S Cell Signaling; 1:500 STAT1 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated at room temperature for 1 h with plant monoclonal anti-actin primary antibodies (CusAb, 1:1000) and 1 h with anti-mouse IgG HRP-conjugated secondary antibodies (Cell Signaling, 1:3000). Membranes were washed and developed as described above.
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bioRxiv - Microbiology 2020Quote: ... incubated O/N in primary antibody (Rabbit Pan-ADPr 1:1000, Cell Signaling E6F6A; Rabbit GFP 1:1000, Chromotek PABG1-100; Mouse Tubulin 1:1000; Cell Signaling DM1A). Primary incubation was followed with HRP-conjugated secondary antibodies (Rabbit-HRP 1:10000 ...
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bioRxiv - Physiology 2021Quote: ... Membranes were blocked using Odyssey blocking buffer and incubated in primary antibodies overnight (αTubulin, 1:1000, Cell Signaling; SIRT3, 1:1000, Cell Signaling; MCAD 1:1000, Cell Signaling; GCN5L1 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000, #2231), AKT (Ser473, 1:1000, #9271), ERK (Thr202/Tyr204, 1:2000, #4370) and STAT3 (Tyr705) (1:1000, #9131, Cell Signaling, Massachusetts, USA) and total EGFR (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated with primary antibodies for drebrin (1:1000, Enzo, Cat#ADI-NBA-110) and GLUA1 (AMPA receptor 1, GLUA1, 1:400, Cell Signaling Cat#13185) in blocking buffer overnight at 4 °C ...
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bioRxiv - Physiology 2023Quote: Antibodies directed against SIK3 (#39477, 1:1000), Myc (#2278 and #2276, 1:1000) and acetylated lysine (#9441, 1:1000) were obtained from Cell Signaling Technology (CST). Antibodies against UCP1 (ab23841 ...
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bioRxiv - Developmental Biology 2023Quote: ... MoBU-1, mouse, 1:250, ThermoFisher #B35128; secondary antibody: anti-mouse IgG Alexa Fluor® 555 Conjugate, 1:500, goat, Cell Signaling #4409). First ...
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bioRxiv - Cell Biology 2023Quote: ... non-phospho (Active) β-Catenin (D13A1, 1:1000 WB), GAPDH (14C10, 1:10000 WB) and YAP (D8H1X, 1:200 IF) were from Cell Signaling Technologies. β-catenin antibody (14 ...
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bioRxiv - Cell Biology 2020Quote: ... The primary antibody used was early endosome antigen 1(EEA1 – 1:200; Cell Signaling) and the secondary antibody was Alexa647 AffiniPure donkey anti-rabbit IgG (1:600 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with antibody (Drosha, Cell Signaling, 1:50 or Safb1, Abcam 1:50 resp.) on a rotating wheel overnight at 4°C (where 2.5% of each sample had been saved as input) ...
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bioRxiv - Molecular Biology 2021Quote: ... p-extracellular signal-regulated kinase (ERK) 1/2 (Cell Signaling Technology, #4370, 1:500), ERK1/2 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-Myc 9B11 at 1:2000-1:3000 (Cell Signaling Technology, Danvers, MA), mouse anti acetylated α-tubulin 6-11B-1 Alexa488 conjugated 1:50 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-E-cadherin (clone 24E10, 3195S, Cell Signaling; IB 1:1000; IHC 1:400), anti-N-cadherin (14215S ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were anti-Cofilin-1 mAB (clone D3F9, 1:100, Cell Signaling, 5175), anti-GRP75 mAB (clone N52A/42 ...
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bioRxiv - Neuroscience 2020Quote: ... Flotillin-1 rabbit polyclonal antibody (D2V7J, Cell Signaling, Cat# 18634, 1:1K for WB); β-actin mouse monoclonal antibody (AC-74 ...
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bioRxiv - Immunology 2020Quote: ... anti-total-NF-κB p65 (1:1□000 dilution, 6956T, Cell Signaling Technology, USA), anti-phospho-TRAF-associated NF-κB activator (TANK)-binding kinase 1 (TBK1 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... was used for experiment 1 while a monoclonal rabbit antibody (1:5000, Cell signaling) was used for experiment 4 ...
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bioRxiv - Pathology 2020Quote: ... Superoxide dismutase (SOD)-1-specific mouse monoclonal antibody (1:1000 dilution, 71G8, Cell Signaling), Heat shock protein (HSP)-60-specific rabbit monoclonal antibody (1:1000 dilution ...