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Citations for Cell Signaling Technology :
1751 - 1800 of 1838 citations for 6 Bromo 2 piperidin 4 yl 1H benzoimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with the following primary antibodies: phospho-p44/42 MAPK (EER1/2) (T202/Y204) (Cell Signaling Technology, Cat. #: 9101, 1:1000), p44/42 MAPK (ERK1/2 ...
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bioRxiv - Plant Biology 2021Quote: ... The phosphorylated MAPK3 and MAPK6 proteins were detected using polyclonal anti-phospho-p44/42 MAPK (Erk1/2, 1:1000; Cell Signaling Technology), and the detection of the total MAPK3 and 6 protein levels were by using polyclonal anti-MAPK3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse brain lysates or cell lysates (1-2 mg) with protease inhibitor cocktail were incubated with anti-MeCP2 (Cell Signaling Technology, 3456) or anti-OGT (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were incubated for 2 hours at room temperature with the primary antibodies 1:1000 anti-HA (C29F4) Rabbit monoclonal antibody (mAb) from Cell Signaling Technology® ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were probed with rabbit monoclonal antibodies directed against the diphosphorylated (Thr202/Tyr204) form of ERK1/2 (p42/44 MAPK, Cat. No. 4372, Cell Signaling Technology) and total ERK1/2 (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356) (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (Cell signaling) as loading control ...
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bioRxiv - Plant Biology 2020Quote: ... or phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (anti-phospho-TEY, #9101, 1:200, Cell Signaling Technology, Danvers, MA, USA). Membranes were washed 3 times for 5 min with TBST and incubated for 1 h with the appropriate secondary antibody – goat anti-rabbit (1:20,000 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were spun down and washed three times with cold Flow Wash Buffer (FWB) (PBS, 2% FBS, 2mM EDTA) and incubated with primary antibody (EGFR-D1D4J, Cell Signaling Technology) diluted in FWB for 60 minutes on ice ...
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bioRxiv - Microbiology 2022Quote: ... cells were blocked with 5% milk in PBS for 1 h and then incubated with α-SARS-CoV-2 nucleocapsid antibody (1:3,000; #26369 Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein electrophoresis and Western-Blot were performed as described previously using the Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology) (1:1000 ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Cancer Biology 2024Quote: ... anti-phospho-p44/42 MAPK T202/Y204 (p-ERK 1/2, #9101, 1:1000), anti-PARP (#9532, 1:1000), and anti-LAMP1 (#9091, 1:400) from Cell Signaling Technologies and anti-Myc-Cy3 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... was done overnight at 4°C and was followed by incubation with anti-mouse IgG (H+L) F(ab’)2 conjugated to Alexa Fluor 647 (Cell Signaling Technology). Images were acquired with ZEISS LSM 980 laser-scanning confocal microscope and analysed using ZEN software (Carl Zeiss MicroImaging).
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice in 1 ml FACS buffer and resuspended in 50-100 μl of 1:50 diluted primary PE-conjugated antibodies against phosphorylated p38 or Erk1/2 (Cell Signaling Technology) and stained for 1 h at room temperature on a rotor ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies to EGR1, EGR3, phospho-ERK1/2, GAPDH and actin, and an inhibitor of EphA2 (dasatinib, 100 nM) were from Cell Signaling Technologies. Secondary HRP-conjugated secondary antibodies used were goat anti-rabbit (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 1–2 hours at room temperature with the following primary antibodies: anti-E-cadherin antibody (Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PKM 1/2 (C103A3, #3190), anti-PKM2 (D78A4, #4053), and anti-pY849 PDGFRα/pY857PDGFRβ (C43E9, #3170S) antibodies were purchased from Cell Signaling Technology. Rabbit monoclonal anti-GLUT1 (ab115730) ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then washed with 1X PBS (2 times, 5 minutes each) and incubated with the primary antibody (COL1A1, Cell signaling #72026S) at 1:50 dilution for 24h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated using a 10% acrylamide SDS-PAGE gel and immunoblotted with anti-phospho-p44/42 MAPK (Erk1/2) (anti-pMAPK; Cell Signaling Technology).
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of SARS-CoV-2 nucleocapsid protein (NP) (E8R1L) fragmented mouse monoclonal antibody f-mAb (Cell Signaling Technology Inc., Massachusetts, USA) are introduced to the sensor surface after diluting in PBS and incubated for 2 hours at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... The membrane was blotted with 1:1,000 dilutions of antibodies for phospho-p44/42 MAPK (phospho-Erk1/2; Cell Signaling Technology #9101), p44/42 MAPK (Erk1/2 ...
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bioRxiv - Genetics 2019Quote: ... the membranes were incubated for 12 hours or longer at 4°C with rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 1:1000 dilution) or mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked for 10 minutes in 10% BSA in TBS-T and then incubated overnight at 4°C with the indicated antibodies obtained from Cell Signaling Technology (phospho-PKC Substrate Motif (#6967), phospho-p42/44 MAPK (#4370) ...
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bioRxiv - Neuroscience 2022Quote: ... At least 3 independent controls and treated samples were probed with the following primary antibodies (in 5% milk, overnight at 4°C): YAP (1:500, Cell signaling Tec., Cat. No. 14074, RRID: AB_2650491), p YAP (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked in 5% evaporated milk in Tris-based saline with Tween 20 (0.05% TBST) for 1 hour and followed by incubating with primary antibodies overnight at 4 °C (Anti-HA: Cell Signaling Technology, 3724S; anti-GAPDH: Thermo, MA5-15738). After three rinses with TBST ...
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bioRxiv - Cancer Biology 2021Quote: ChIPs were performed using cross-linked chromatin from Capan-2 cells and either anti-BCL6 antibodies (1;1000, Cell Signaling Technology Cat# 14895, RRID:AB_2798638), anti-STAT1 antibodies (1;1000 ...
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bioRxiv - Microbiology 2021Quote: ... Antibody labelling was performed with mouse anti-SARS-CoV-2 S protein (Abcalis, clone AB68-A09, #ABK68-A09-M) and secondary antibody anti-mouse Alexa488 (Cell Signaling Technology, #4408), each step followed by three washing steps with PBS containing 0.05% Tween-20 ...
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... Co-IPs of endogenous proteins was performed using 500 μgr of PNS incubated with 2 μg of anti-MDM2 or 1 μg of anti-VHL (Cell Signaling technology,68547). The following antibodies were used Anti-GFP ab1218 Abcam (Ip) ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for Western Blot [Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody #9101] were procured from Cell Signaling Technology (Danvers, MA, USA). Antibodies for flow cytometry were procured from BioLegend and eBioscience (San Diego ...
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bioRxiv - Microbiology 2020Quote: ... MAP kinase activation was detected by western-blot analysis with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling Technology, 9101S) and anti-rabbit horseradish peroxidase-linked sheep antibody ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... cells were collected by centrifugation at 500 × g for 5 minutes and resuspended in 1 ml TNE buffer (150 mM NaCl, 2 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease and phosphatase inhibitors (Cell Signaling Technology, Danvers, MA), passed through a 25 gauge needle to shear the cells ...
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bioRxiv - Biochemistry 2021Quote: ... Anti-mouse IgG (H+L) and F(ab’)2 Fragment Alexa Fluor® 555-conjugate (#4409) were purchased from Cell Signaling Technologies (CST). Mouse monoclonal (M2 ...
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bioRxiv - Physiology 2019Quote: ... phospho-p44/42 MAPK Thr202/Tyr204 antibody (rabbit, 1:2000) and Erk1/2 antibody (rabbit, 1:2000; all from Cell Signaling Technology, USA), and a-tubulin antibody (mouse,1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... then stained with Hoechst 33342 (2 µg/mL) in OBB and antibodies against p-ERK (1:200, Cell Signaling Technologies, Cat. No. 4370S), then anti-rabbit IgG (1:2000 ...
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bioRxiv - Physiology 2021Quote: ... anti-phospho and total extracellular signal-regulated kinase antibody (p/total ERK 1-2 1:1000 CST4396, CST4696, Cell Signaling Technology, Danvers, MA), anti-phospho and total protein kinase B antibody (AKT 1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... was analyzed for MAPK phosphorylation by immunoblotting using the primary antibody anti-pERK MAPK (Phospho-p44/p42 MAPK, ERK1/2,Thr202/Tyr204, D13.14.4E; Cell Signaling Technology, Danvers, MA, USA) at a dilution of 1:2,500 and the secondary antibody (Goat anti-rabbit IgG (H+L)-HRP conjugate ...
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bioRxiv - Immunology 2023Quote: ... and equal volume of 2X STOP Buffer (Buffer 2 + 20uM EDTA + 4uM EGTA) was added along with 1 pg of Saccharomyces cerevisiae spike-in DNA (Cell Signaling, cat. 29987). Samples were incubated for 15 mins at 37°C and DNA was isolated and purified using Qiagen MinElute Kit according to manufacturer’s protocol and subjected to library amplification50,51.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti phospho-myosin light chain 2 Thr18/Ser19 (pMLC, 1:50 for IF, 1:20 for IG) was from Cell Signaling Technologies (#3674). Chicken anti-map2 (against residues 2-314 of human map2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... phospho-ERK1/2 (D13.14.4E; Cat. #4370) and mouse antibodies again ERK1/2 (L34F12; Cat. #4696) and β-Actin (8H10D10; Cat. #3700) are from Cell Signaling Technology. Rabbit anti-Cytokeratin 10 (Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked with TBS containing 2% (m/v) bovine serum albumin (BSA) and then incubated with antibodies raised against the HA epitope (Cell Signaling Technology, USA) diluted 1:1000 in the same solution ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...