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Citations for Cell Signaling Technology :
1701 - 1750 of 1838 citations for 6 Bromo 2 piperidin 4 yl 1H benzoimidazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Physiology 2021Quote: ... were subjected to western blot analysis with antibodies against c-Jun NH(2)-terminal kinase (JNK) and phospho-JNK (Cell Signaling). Immunocomplexes were detected using the Licor Odyssey secondary detection system.
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bioRxiv - Cancer Biology 2021Quote: ... the degree of staining of the tissue specimens was observed under the Zeiss Apotome.2 microscope (20X objective, 0.8 NA) after DAB staining (Cell Signaling Technology) and hematoxylin (IHC World ...
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bioRxiv - Systems Biology 2020Quote: ... from femur by incubating the samples with mouse anti-Runx-2 (an osteoblast marker) antibody (1:1000 dilution in PBS containing 0.5% BSA, Cell Signaling Technology) at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Primary antibody solution mix contains two primary antibodies raised in two different species (mouse and rabbit) targeting the proteins of interest: rabbit anti-mitofusin 2 (1:50, mAb D2D10, Cell Signaling), rabbit anti-VDAC (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with rabbit antibodies against phospho (S473)-AKT (p-AKT) and phospho (Thr202/Tyr204) p44/42 ERK1/2 (p-ERK) (4060, 9101; Cell Signaling) for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were blocked with 3% BSA and incubated with primary antibodies against pERK1/2 (1:500, Cell Signaling Technology, 4370) or cleaved Caspase-3 (1:400 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... Phosphorylated MAPK proteins were detected by immunoblot analysis with antiphospho-p44/42 MAPK (Erk1/2)(Thr202/Tyr204) (D13.14.4E) rabbit mAb (Cell Signaling Technology, USA). The blotted membrane was stained with Coomassie Brilliant blue (CBB ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was blocked with 5% non-fat dry milk in TBST and incubated with primary antibodies against Perilipin-2 (1:5000 gift from Dr. Ronal Kuhnlein) 13 and Actin (1:1000, Cell Signaling). The western blot images were captured by a BIO-RAD workstation.
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2000, Cell Signaling Technology, Danvers, MA, USA,; RRID: AB_2315112), rabbit polyclonal anti-human SOD1 (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Double resistant cell lines with dual integration of 4EBP1/2 shRNAs were validated by western blot (Cell Signaling Technology #9452, #2845) for efficient stable knockdown of the targeted proteins.
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bioRxiv - Plant Biology 2023Quote: ... Blots for detecting phosphorylated MAPK were blocked in 5% BSA and immunoblotted with a phosphor-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cell Signaling). Horseradish peroxidase-conjugated anti-rabbit antibody produced in goat (Agrisera ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immunolabeling was realized using the rabbit monoclonal primary antibody anti-phospho-ERK1/2 Thr202/Tyr204 (#4370, Cell Signaling Technologies, 1:2,000), or anti-total ERK1/2 (#05-1152 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... anti-phospho-Akt (Thr308) (C31E5E) and anti-phospho-p44/42 MAPK (Erk 1/2) (Thr202/Tyr204) (E10) antibodies (Cell Signaling Technology). Each experiment was repeated 4 times ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000, 71G4B7, Cell Signaling; beta actin 1:5000 sc-4778 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein-specific IgG was measured using SARS-CoV2 spike protein serological IgG ELISA kit (Cell Signaling Technology) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were incubated overnight at 4°C on a rocker with primary antibodies (Supplemental Table 2) according to the manufacturer’s instructions and then developed with HRP-conjugated secondary antibodies (Cell Signaling Technology) for 1 hour at room temperature on an orbital shaker ...
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bioRxiv - Molecular Biology 2023Quote: ... The antibody incubation was carried out overnight on a rotor at 4ºC in 100 μL antibody solution (wash buffer with 0.1% digitonin and 2 mM EDTA) containing 1 μL of αH3K27me3 Ab (Cell Signaling C36B11). Samples were then washed in dig-wash buffer (wash buffer with 0.1% digitonin) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then permeabilized and blocked with 0.1% saponin/0.1% BSA in PBS before incubated with α-SARS-CoV-2 Nucleocapsid primary antibody (Cell Signaling Technology) at 1:1000 in permeabilization/blocking buffer overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were blocked with 2% BSA in PBS for 1 hour at room temperature before antibody staining with rabbit ⍺-Sec61b (Cell Signaling) and mouse ⍺-Tom20 (Santa Cruz ...
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bioRxiv - Neuroscience 2024Quote: ... every 8th section of the brain was processed for double-fluorescent immunostaining for Ku80 and one of the following: Microtubule-associated protein 2 (MAP2; 8707, Cell Signaling), doublecortin (ab18723 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for viral protein analysis: SARS-CoV-2 anti-Spike and anti-N protein (cat #569996 and #33336, respectively, Cell Signaling) and anti-β-actin (Sigma – cat #a2228) ...
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bioRxiv - Cancer Biology 2019Quote: ... blocked in 8% goat serum and incubated overnight at 4°C with anti-phospho Axl (R&D, AF2228, diluted 1:200) CK11 (Cell signaling, CST 4545, diluted 1:200), followed by fluorescently labelled secondary antibodies goat anti mouse 488 (Abcam ab98637) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... the blot was incubated at 4 °C with primary antibodies (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:3,000 dilution) (rabbit anti-Calnexin, Cell Signaling, Cat #: 4691, 1:2,000 dilution) (rabbit anti-COX4 ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were carried out on the remaining sample overnight at 4 °C with rotation with 5 uL of antibody (Cell Signaling Technology D31H2 anti-CTCF, 3418S). 50uL of Protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Supernatant was transferred to new tubes and antibody was added and allowed to bind overnight at 4 °C with rotation (15uL antibody per tube, Cell Signaling Technology D31H2 anti-CTCF, 3418S). 30uL of pre-washed Protein G Dynabeads were added to each tube and rotated at 4 °C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg/μl (500 μg protein) samples were prepared and incubated overnight at 4 °C in primary antibody against acetyl-lysine (Cell Signaling; Cat No. 9814 (1:100)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The membranes were probed overnight at 4 °C with the specified primary antibodies at the dilution of 1:1000 (Cell Signaling Technology: RIPK1 Rabbit mAb#3493)) or 1:4000 (β-actin Rabbit mAb#4970)) ...
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bioRxiv - Cancer Biology 2024Quote: ... then incubated with primary antibody diluted in 1% FBS/PBS overnight at 4°C (MAP2, Cell Signaling # 4542, 1:500 dilution; NEFH, Cell Signaling # 55453, 1:500 dilution). After 3 washes with PBS ...
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bioRxiv - Plant Biology 2020Quote: ... membranes were incubated with the antibody against Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® (Cell Signaling Technology, USA). As a loading control membranes were incubated with anti-tubulin antibody (Abiocode ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% milk and incubated in 2% BSA with primary antibodies to GAPDH (Cell Signaling Technology Cat# 13084, RRID:AB_2713924), Eg5 (Sigma Aldrich Cat# SAB4501650 ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then blotted overnight with anti-human phospho-Akt and anti-human phospho-Erk1/2 mAbs (Cell Signaling, MA, USA), followed by anti-rabbit or anti-mouse horseradish peroxidase(HRP)-conjugated secondary mAb (ThermoFisher scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were permeabilized with 0.1% Triton-X and stained for intracellular phospho-p44/42 Erk1/2 Thr202/Tyr204 at 1:700 dilution (Cell Signaling Technology, 4370) and p44/42 Erk1/2 at 1:700 dilution (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet containing the DOC-insoluble material was resuspended in 100 μl SDS solubilization buffer, pH8.8 (20 mM Tris-Cl, 2 mM EDTA, 1% w/v SDS, 1X protease inhibitor cocktail (Cell Signaling Technology, 5871). The DOC-insoluble pellet was thoroughly dissolved by heating the sample to 95 °C and vortexing ...
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bioRxiv - Neuroscience 2021Quote: ... Then the cells were blocked in 10% donkey serum for 2 h and incubated with primary antibodies (goat anti-Iba1, 1:400, Abcam; mouse anti-CD68, 1:400, Cell Signaling Technology; rabbit anti-Arg-1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Nuclei pellets were washed twice with 200 μL of Dig-wash buffer before resuspending in 100 μL Dig-wash buffer supplemented with 2 mM EDTA and a 1:50 dilution of H3K27ac primary antibody (Cell Signaling, 8173) and incubated overnight at 4°C on an overhead rotator ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-ERK1/2 (dilution 1:2000) and rabbit α-tubulin (dilution 1:2000) all purchased from Cell Signaling Technology (Danvers, MA). The phospho-ERK1/2 expression was evaluated first using a 680-nm secondary anti-mouse antibody ...
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bioRxiv - Cancer Biology 2020Quote: ... discs were washed 5 times for a total of 30 minutes with 0.1% PBST and incubated overnight in goat anti-mouse F(ab)’2 AlexaFluor-555 secondary antibody (Cell Signaling, 1:500). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...