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• Increased EZH2 function in regulatory T cells promotes their capacity to suppress autoimmunity by driving effector differentiation prior to activation

  Janneke G.C. Peeters, et al., bioRxiv - Immunology 2024

  Quote: ... anti-histone H3 (D1H2, Cell Signaling Technology), anti-tri-methyl-histone H3 (Lys27 ...
• Increased EZH2 function in regulatory T cells promotes their capacity to suppress autoimmunity by driving effector differentiation prior to activation

  Janneke G.C. Peeters, et al., bioRxiv - Immunology 2024

  Quote: ... or normal rabbit IgG (#2729, Cell Signaling Technologies). Permeabilized cells were washed and incubated with pA-MNase (kindly provided by the Henikoff lab ...
• Increased EZH2 function in regulatory T cells promotes their capacity to suppress autoimmunity by driving effector differentiation prior to activation

  Janneke G.C. Peeters, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were incubated rotating for 2 hr at 4°C with 1 uL anti-tri-methyl-histone H3 (Lys27) (C36B11, Cell Signaling Technology) or normal rabbit IgG (#2729 ...
• Gene-specific RNA homeostasis revealed by perturbation of the NuA4/Tip60 acetyltransferase complex

  Faezeh Forouzanfar, et al., bioRxiv - Genomics 2024

  Quote: ... incubated in HRP-conjugated anti IgG secondary antibodies (Cell Signaling Technology) in TBSTM at room temperature ...
• Dbp1 is a low performance paralog of RNA helicase Ded1 that drives impaired translation and heat stress response

  Emily N Powers, et al., bioRxiv - Genomics 2024

  Quote: ... Blots were incubated with a rabbit anti FLAG antibody (1:1000, RRID; AB_2217020, 2368, Cell Signaling Technology), rabbit anti-hexokinase antibody (1:10,000 ...
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... IKKα (1:2000, Cell Signaling Technology, 2682S), IKKβ(1:2000 ...
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... CYLD (1:2000, Cell Signaling Technology, 8462S), β-actin (1:10000 ...
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... HSP90 (1:5000, Cell Signaling Technology, 4874S).
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... p-IKKα/β (1:2000, Cell Signaling Technology, 2697S), IKKα (1:2000 ...
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... GFP (1:1000, Cell Signaling Technology, 2956S), HSP90 (1:5000 ...
• Caspase-8-mediated CYLD Cleavage boosts LPS-induced Endotoxic Shock

  Jianling Liu, et al., bioRxiv - Immunology 2024

  Quote: ... p-IkBα (1:1000, Cell Signaling Technology, 9246S), p-P65 (1:2000 ...
• A robust platform for BaEVRless-lentiviral synthesis and primary natural killer cell transduction

  Yi-Jun Lan, et al., bioRxiv - Immunology 2024

  Quote: ... anti-ASCT2 (#8057, Cell Signaling Technology), anti-rabbit IgG (#7074 ...
• IL-10 dependent adaptation allows macrophages to adjust inflammatory responses to TLR4 stimulation history

  H. Bongartz, et al., bioRxiv - Immunology 2024

  Quote: ... Alexa Fluor™ 647-conjugated serine 536-phosphorylated NFκB p65 (4887) and PE-conjugated NFκB1 p105/p50 (24961) antibodies were obtained from Cell Signaling Technology. PE-CF594-conjugated threonine 180- and tyrosine 182-phosphorylated p38-MAPK (563569 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-IRF3 p-S386 (1:1000, 37829S, Cell Signaling Technology), anti-RFP (1:2,000 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit anti-Src antibody (1:1000, 2109S, Cell Signaling Technology), mouse anti-Src antibody (1:1000 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... anti- Mx1 (1:1,000, 37849S, Cell Signaling Technology), and anti-FLAG (1:40,000 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... and HRP-linked anti-mouse IgG (1:2000, 7076S, Cell Signaling Technology). Western blots were developed with the SuperSignal West Pico PLUS Chemiluminescent Substrate Kit (Thermo Fisher Scientific ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... Primary antibodies utilized in this study are as follows: anti-GAPDH (1:5,000, 2118S, Cell Signaling Technology), anti-ALTO (1:10,000 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: The secondary antibodies utilized in this study are HRP-linked anti-rabbit IgG (1:2000, 7074S, Cell Signaling Technology), and HRP-linked anti-mouse IgG (1:2000 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-P65 p-S536 (1:1000, 3033S, Cell Signaling Technology), anti-IRF3 (1:500 ...
• Merkel cell polyomavirus protein ALTO modulates TBK1 activity to support persistent infection

  Ranran Wang, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-STING (1:1000, 13647S, Cell Signaling Technology), anti-STING p-S366 (1:1000 ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... p-TBK1 rabbit mAb (Cell Signaling Cat# 5483S), TRAF2 rabbit pAb (Proteintech Cat# 26846-1-AP) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... Phospho-STING rabbit anti-mouse mAb (Cell Signaling Cat# 72971S), IRF3 rabbit mAb (Cell Signaling Cat# 4302S) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... p-IRF3 rabbit mAb (Cell Signaling Cat# 4947S), TBK1 rabbit mAb (Cell Signaling Cat# 3504S) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... IRF3 rabbit mAb (Cell Signaling Cat# 4302S), p-IRF3 rabbit mAb (Cell Signaling Cat# 4947S) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... and Phospho-Ubiquitin rabbit mAb (Cell Signaling Cat #70973).
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... Antibodies used for western blotting are: STING Rabbit mAb (Cell Signaling Cat# 13647S), Phospho-STING rabbit anti-mouse mAb (Cell Signaling Cat# 72971S) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... TBK1 rabbit mAb (Cell Signaling Cat# 3504S), p-TBK1 rabbit mAb (Cell Signaling Cat# 5483S) ...
• ZFAND6 is a subunit of a TRAF2-cIAP E3 ubiquitin ligase complex essential for mitophagy

  Kashif Shaikh, et al., bioRxiv - Immunology 2024

  Quote: ... HSP60 rabbit mAb (Cell Signaling Cat# 12165) and Phospho-Ubiquitin rabbit mAb (Cell Signaling Cat #70973).
• The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness

  Xingxing Zhu, et al., bioRxiv - Immunology 2024

  Quote: ... and anti-RagA (D8B5) all from Cell Signaling Technology) ...
• The nutrient-sensing Rag-GTPase complex in B cells controls humoral immunity via TFEB/TFE3-dependent mitochondrial fitness

  Xingxing Zhu, et al., bioRxiv - Immunology 2024

  Quote: ... followed by staining with anti-phospho-S6 (S235/236) or anti-phospho-4E-BP1 (T37/46) (both from Cell Signaling Technology) for 30 min at room temperature ...
• Type I interferon alters invasive extravillous trophoblast function

  Michael K. Simoni, et al., bioRxiv - Immunology 2024

  Quote: ... the cells were incubated with primary antibodies against Caspase-3 (Asp175, Cell Signaling, catalog # 9661), PECAM1 (2HB ...
• Single-cell image-based genetic screens systematically identify regulators of Ebola virus subcellular infection dynamics

  Rebecca J. Carlson, et al., bioRxiv - Microbiology 2024

  Quote: ... c-Jun (1:1800 dilution, Cell Signaling Technology Cat# 9165, RRID:AB_2130165), and vimentin (1:1300 dilution ...
• Microbial Metabolite Trimethylamine N-Oxide Promotes Campylobacter jejuni Infection by Escalating Intestinal Inflammation, Epithelial Damage, and Barrier Disruption

  Caitlyn B. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... zonula occludens-3 (ZO-3) (Cell Signaling Technologies), anti-mouse IgG (Alexa Fluor® 488 ...
• HIV-1 Vpr causes separate cell cycle arrests in G2 and M that activate alternative DNA damage pathways

  Ross Hall, et al., bioRxiv - Microbiology 2024

  Quote: ... or BI2536 (#26744, Cell Signaling Technology, 100 nM) for 15h ...
• Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4⁺ T cells through STAT1-related epigenetic mechanisms

  Jie Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
• Thymic dendritic cell-derived IL-27p28 promotes the establishment of functional bias against IFN-γ production in newly generated CD4⁺ T cells through STAT1-related epigenetic mechanisms

  Jie Zhang, et al., bioRxiv - Immunology 2024

  Quote: ... and STAT1 (Cell Signaling Technology).
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-pSTAT3 (Ser727) (1:500, #9134, Cell Signaling Technology), anti-STAT3 (1:1000 ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-pSTAT3 (Tyr705) (1:500, #9131, Cell Signaling Technology), anti-pSTAT3 (Ser727 ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-rabbit IgG (1:5000, #7074, Cell Signaling Technology), anti-mouse IgG ...
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... (1:4000, #7076, Cell Signaling Technology).
• LRRK2 G2019S mutation suppresses differentiation of Th9 and Treg cells via JAK/STAT3

  Ningbo Zheng, et al., bioRxiv - Immunology 2024

  Quote: ... anti-STAT3 (1:1000, #9139, Cell Signaling Technology), anti-rabbit IgG (1:5000 ...
• Fes-deficient macrophages enhance CD8⁺ T cell priming and tumour control through increased proinflammatory cytokine production and presentation

  Brian J. Laight, et al., bioRxiv - Immunology 2024

  Quote: ... phospho-SHP2 (1:1000; Cell Signaling Technologies cat#:5431s), γ-tubulin (Sigma-Millipore ...
• Fes-deficient macrophages enhance CD8⁺ T cell priming and tumour control through increased proinflammatory cytokine production and presentation

  Brian J. Laight, et al., bioRxiv - Immunology 2024

  Quote: ... phospho-NF-κB (1:1000; Cell Signaling Technologies cat#: 3033S), phospho-TBK1 (1:1000 ...
• Fes-deficient macrophages enhance CD8⁺ T cell priming and tumour control through increased proinflammatory cytokine production and presentation

  Brian J. Laight, et al., bioRxiv - Immunology 2024

  Quote: ... and immunoblotting was performed as previously described using the following antibodies and concentrations: Fes (1:1000; Cell Signaling Technologies cat#: 85704S), phospho-NF-κB (1:1000 ...
• Fes-deficient macrophages enhance CD8⁺ T cell priming and tumour control through increased proinflammatory cytokine production and presentation

  Brian J. Laight, et al., bioRxiv - Immunology 2024

  Quote: ... phospho-TBK1 (1:1000; Cell Signaling Technologies cat#: 5483), phospho-SHP2 (1:1000 ...
• Lysosomal integrity suppresses TIR-1/SARM1 aggregation to restrain toxic propagation of p38 innate immunity

  Samantha Y. Tse-Kang, Read Pukkila-Worley, bioRxiv - Immunology 2024

  Quote: ... 1:1000 phospho p38 PMK-1 (Cell Signaling Technology, #9211), 1:1000 total PMK-1,23 1:2000 mouse monoclonal anti-alpha-Tubulin (Sigma ...
• Lysosomal integrity suppresses TIR-1/SARM1 aggregation to restrain toxic propagation of p38 innate immunity

  Samantha Y. Tse-Kang, Read Pukkila-Worley, bioRxiv - Immunology 2024

  Quote: ... anti-rabbit IgG-HRP (Cell Signaling Technology, #7074), or anti-rat IgG-HRP (Abcam ...
• Endosomal LRBA regulates the endo-lysosomal pathway

  Viktória Szentgyörgyi, et al., bioRxiv - Immunology 2024

  Quote: ... rabbit polyclonal anti phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (#9101, Cell Signaling, 1:1000), mouse anti calnexin antibody clone 37 (#610523 ...
• Chitinase 3-like-1 (CHI3L1) Inhibits Innate Anti-Tumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα and CD24-Siglec10-Mediated Phagocytosis

  Bing Ma, et al., bioRxiv - Immunology 2024

  Quote: ... and SHP-2 (Cell Signaling Technology, #3752) overnight at 4°C in TBST and 5% BSA ...