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• Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

  Dong Wang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... MYC (Cell signaling, 13987S, 1:50), Pol II (Cell signaling ...
• Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

  Dong Wang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The antibodies used for cu and run were CDK8 (Cell signaling, 4101S, 1:50), MYC (Cell signaling ...
• Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

  Dong Wang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Pol II (Cell signaling, 2629S,1:50), phospho-Pol II (cell signaling ...
• Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

  Dong Wang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... H3K27me3 (Cell signaling, 9733s, 1:50), and IgG (EpiCypher ...
• Transcriptional Regulation of Protein Synthesis by Mediator Kinase in MYC-driven Medulloblastoma

  Dong Wang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... p-S6-S235/236 (Cell Signaling, 2211S), p-AKT-S473 (Leica ...
• Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal

  William J. Cisneros, et al., bioRxiv - Microbiology 2024

  Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
• Release of P-TEFb from the Super Elongation Complex promotes HIV-1 latency reversal

  William J. Cisneros, et al., bioRxiv - Microbiology 2024

  Quote: ... CCNT1 antibody (Cell Signaling Technologies, Cat#81464) was then added to precleared lysates at 1:100 and incubated with rotation overnight at 4°C ...
• Addressing anemia severity in antimony-resistant Leishmania donovani infection at the nexus of oxidative outburst and iron transit

  Souradeepa Ghosh, et al., bioRxiv - Microbiology 2024

  Quote: ... GAPDH (D16H11) and β-actin were purchased from Cell Signaling Technologies (CST). Anti-Mouse CD11b-Alexa Fluor 488 is purchased from Invitrogen (53-0112-82) ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: The following antibodies were used: anti-eIF2α (1:1000, Cell Signaling Technology, #9722), anti-phospho-eIF2α Ser51 (1:1000 ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-PARP (1:1000, Cell Signaling Technology, #9532), anti-GAPDH (1:4000 ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-RPS3 (1:1000, Cell Signaling Technology, #9538), anti phospho- histone H3 (Ser10 ...
• Coronavirus Nucleocapsid Protein Enhances the binding of p-PKCα to RACK1: Implications for Inhibition of Nucleocytoplasmic Trafficking and Suppression of the Innate Immune Response

  Wenxiang Xue, et al., bioRxiv - Microbiology 2024

  Quote: ... Okadaic Acid (#5934) was purchased from Cell Signaling Technology, USA ...
• Transcriptional cascades during fasting amplify gluconeogenesis and instigate a secondary wave of ketogenic gene transcription

  Dana Goldberg, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and incubated for 16 h with primary antibody (PPARα - Santa Cruz Biotechnology cat# sc-398394, H3 - Cell Signaling Technologies cat# 14269 ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... 1% (vol/vol) Triton X-100) or RIPA buffer (Cell Signaling) and cleared by centrifugation ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Cathepsin B (CTSB, 1:1000, Cell Signaling, rabbit, #31718) RRID ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Citrate Synthase (CS, 1:1000, Cell Signaling, rabbit, #14309) RRID:AB_2665545 ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... GAPDH (1:1000, Cell Signaling, rabbit, #5174) RRID ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Calreticulin (CALR, 1:1000, Cell Signaling, rabbit, #12238) RRID ...
• Human MX1 orchestrates the cytoplasmic sequestration of neo-synthesized influenza A virus vRNPs

  Joe McKellar, et al., bioRxiv - Microbiology 2024

  Quote: ... Rab11 (Cell Signaling, 5589 1/50), Rab11a (Proteintech ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-eIF1 (1:1000, Cell Signaling Technology, #12496), anti-eIF1 (1ug/mL ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti-phospho-eIF2α Ser51 (1:1000, Cell Signaling Technology, #3398), anti-eIF1 (1:1000 ...
• Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology

  Jimmy Ly, et al., bioRxiv - Molecular Biology 2024

  Quote: ... anti phospho- histone H3 (Ser10) (1:1000, Cell Signaling Technology, #9701), anti-CDC20 (1:200 ...
• Inhibition of eukaryotic translation initiation factor 1A (eIF1A) and 3B (eIF3B) diminishes the psoriatic phenotype in two mouse models and human 3D model samples

  Nicole Golob-Schwarzl, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and Ki-67 (1:250; #12202S, Cell Signaling, Massachusetts, USA). As a secondary method ...
• Platelets induce cell apoptosis of cardiac cells via FasL after acute myocardial infarction

  Kim J. Krott, et al., bioRxiv - Molecular Biology 2024

  Quote: ... the following antibodies were used: Cleaved caspase-3 (Casp-3, #9661, Cell Signaling, 1:50), vimentin (#PA5-142829 ...
• Identification and characterization of the de novo methyltransferases for eukaryotic N⁶-methyladenine (6mA)

  Ting Cheng, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and ɑ-HA (Cell Signaling, C29F4, 1:200, rabbit). The secondary antibodies were Goat anti-Rabbit IgG (H+L) ...
• Discovery of a potent inhibitor that suppresses glioblastoma by dual targeting of both syntenin PDZ domains

  Yunseok Heo, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and p65 were obtained from Cell Signaling Technology (USA). Monoclonal antibodies capable of recognizing MDA-9/syntenin ...
• Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG

  Xiaoyun Ding, et al., bioRxiv - Neuroscience 2024

  Quote: ... A rabbit monoclonal antibody against Neurofascin 186 (Clone D6G6O, Cell Signaling Technology Cat# 15034, RRID:AB_2773024, 1:500). Rabbit polyclonal antibodies against Caspr (Abcam Cat# ab34151 ...
• APOE regulates the transport of GM1

  Dong Yan Zhang, et al., bioRxiv - Neuroscience 2024

  Quote: ... and control GAPDH antibody (diluted 1:1000, Cell Signaling Technology). We used anti-rabbit HRP (diluted 1:1000 ...
• The SAGA acetyltransferase module is required for the maintenance of MAF and MYC oncogenic gene expression programs in multiple myeloma

  Ying-Jiun C. Chen, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and HA (Cell Signaling Technology) antibodies overnight at 4°C ...
• The SV40 virus enhancer functions as a somatic hypermutation-targeting element with potential oncogenic activity

  Filip Šenigl, et al., bioRxiv - Microbiology 2024

  Quote: ... Anti-Mouse IgG HRP linked antibody (Cell signaling) 1:3000 dilution in 5%-BSA-TBS was used as secondary antibody.
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... VDAC (1:1000, Cell Signaling, rabbit, #4661) RRID ...
• Ergothioneine boosts mitochondrial respiration and exercise performance via direct activation of MPST

  Hans-Georg Sprenger, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Golgin 97 (1:1000, Cell Signaling, rabbit, #13192) RRID ...
• The Role of Ebola Virus VP24 Nuclear Trafficking Signals in Infectious Particle Production

  Olivia A. Vogel, et al., bioRxiv - Microbiology 2024

  Quote: ... Rabbit anti-HDAC2 (Cell Signaling, cat #2540S).
• DEAD box RNA helicase 5 is a new pro-viral host factor for Sindbis virus infection

  Mélanie Messmer, et al., bioRxiv - Microbiology 2024

  Quote: ... or 2µg rabbit IgG antibody (2729; Cell Signaling Technology).
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... TBK1 (Cell Signaling #38066S, 1:1000), IRF3 (Cell Signaling #4302S ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... cGAS (Cell Signaling #15102S, 1:1000), TBK1 (Cell Signaling #38066S ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... pSTAT1-S727 (Cell Signaling #8826, 1:1000), STAT1 (Cell Signaling #14994 ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... MX1 (Cell Signaling #37849, 1:1000), IFIT1 (Cell Signaling #14769 ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... pSTAT1-Y701 (Cell Signaling #9167, 1:1000), pSTAT1-S727 (Cell Signaling #8826 ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... STING (Cell Signaling #13647S, 1:1000), cGAS (Cell Signaling #15102S ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... STAT1 (Cell Signaling #14994, 1:1000), MX1 (Cell Signaling #37849 ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... IFIT1 (Cell Signaling #14769, 1:1000), STING (Cell Signaling #13647S ...
• A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

  Maritza Puray-Chavez, et al., bioRxiv - Microbiology 2024

  Quote: ... IRF3 (Cell Signaling #4302S, 1:1000), SARS-CoV-2 Nucleocapsid (Sino Biological # 40588-T62 ...
• Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway

  Dar Hershkovitz, et al., bioRxiv - Microbiology 2024

  Quote: ... anti phospho-JNK antibody 4668 (Cell Signaling), or anti phopho-p38 antibody 4631 (Cell Signaling) ...
• Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway

  Dar Hershkovitz, et al., bioRxiv - Microbiology 2024

  Quote: ... or anti phopho-p38 antibody 4631 (Cell Signaling). Filters were scanned by densitometry and the relative phosphorylation level of each MAPK member was determined relative to anti α-tubulin antibody T9026 (Sigma ...
• Genetic evidence for a regulated cysteine protease catalytic triad in LegA7, a Legionella pneumophila protein that impinges on a stress response pathway

  Dar Hershkovitz, et al., bioRxiv - Microbiology 2024

  Quote: ... The other filter was immunoprobed using anti phospho-ERK antibody 9101 (Cell Signaling), anti phospho-JNK antibody 4668 (Cell Signaling) ...
• Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii

  Rodolpho Ornitz Oliveira Souza, et al., bioRxiv - Microbiology 2024

  Quote: ... mouse anti-Myc (9B11 Cell Signaling, 1:1000), rabbit anti-acetyl Tubulin (Lys40 ...
• Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii

  Rodolpho Ornitz Oliveira Souza, et al., bioRxiv - Microbiology 2024

  Quote: ... and anti-beta actin mouse 1:10,000 (8H10D10 cat. no. 3700S) all from Cell Signaling Technology. Anti-TgATOM40 rabbit G579 (1:5000) ...
• Myosin A and F-Actin play a critical role in mitochondrial dynamics and inheritance in Toxoplasma gondii

  Rodolpho Ornitz Oliveira Souza, et al., bioRxiv - Microbiology 2024

  Quote: ... with protease/phosphatase inhibitor cocktail (100×, Cell Signaling Technology). After 1 h of lysis at 4°C ...
• DNA damage drives antigen diversification through mosaic VSG formation in Trypanosoma brucei

  Jaclyn E. Smith, et al., bioRxiv - Microbiology 2024

  Quote: ... F(ab’)2 Fragment (Cell Signaling, 4414S) at 1:1000 at 4C while shaking in PBS + glucose ...