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Citations for Cell Signaling Technology :
1501 - 1550 of 1680 citations for 6 CHLORO 3 METHYLPYRIDO 3 4 D PYRIMIDIN 4 3H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control antibody (Cell Signaling Technology, 1:300), or anti-IRF1 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μg of lysates were incubated overnight at 4 °C on a rotating wheel with an IgG Isotype Control (Cell Signaling Technology, 1:300), anti-Ollas (Novus ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked in 5% milk and incubated overnight at 4 °C in primary antibody (anti-PPARγ, Santa Cruz #sc-7273; or anti-β-actin, Cell Signaling #3700). Washes were performed in TBS-T (20 mM Tris ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were subsequently incubated overnight at 4° C with primary anti-phosphorylated ERK antibody (anti-pERK, Cell Signaling, Cat # 4370, RRID: AB_2315112, 1:400). After primary antibody incubation and PBS wash ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated at 4°C overnight in rabbit anti-c-fos(1:1000 dilution, 2250T Rabbit mAb, Cell Signaling Technolog, Danvers, Massachusetts, USA) primary antibody or mouse anti-TPH2 (1:100 dilution ...
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bioRxiv - Microbiology 2023Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 5% non-fat milk in TBST for 1 h and incubated with the appropriate primary antibody overnight at 4°C: anti-NANOG (D73G4) (Cell Signaling #4903; 1:1000), anti-FAK (D2R2E ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution) or GAPDH-HRP Conjugate (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2023Quote: ... and an equivalent of 1 µg of DNA-normalized DPCs was resolved using SDS-acrylamide gradient gel with the addition of 4 M urea (Cell Signaling, #38650, 1: 1000). DNA detection was performed by applying 2 ng of the sample collected for Pico Green detection to a nylon membrane and detecting with the α-dsDNA antibody (abcam ab27156 ...
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bioRxiv - Microbiology 2023Quote: ... and the remaining volume was rotated overnight at 4 °C with 45 μl of anti-C-myc magnetic beads from Cell Signaling Technology (Cat. 5698) or Pierce (Cat ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated overnight at 4°C with different primary antibodies in TBS-T against LEF-1 (1:500, Antibody# 2230, Cell Signaling Technology, MA, USA), and β-actin (1:30000 ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween 20 and 5% BSA overnight at 4 °C with anti-Runx2 antibody (RUNX2 D1H7 Rabbit mAb, Cell Signaling Technology, 84866 1:2000) and anti-β-actin antibody (Rabbit Anti-beta Actin ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with 200 μl of 1XDPBS + 1% BSA + 0.1% Tween-20 containing 4 μl of Alexa Fluor 488-conjugated phospho-histone H3 (Ser10) antibody (Cell Signaling Technologies, 1:50 dilution) for 90 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The blocked membrane was cut and then incubated with shaking overnight at 4°C with either CD19 antibody #3574 (Cell Signaling; 1:1000 dilution), EAAT3 antibody (Cell Signaling ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with the primary antibodies (Mettl3 Abcam ab195352 1/200; N-Myc Cell Signaling Technology D1V2A 1/200). EU fluorescence coupling was performed following the manufacturer ’s instructions for Click-iT™ RNA Alexa Fluor 488 Imaging Kit ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... the membrane was blocked in 5% nonfat milk in PBST (PBS and 0.1% TWEEN20) and incubated overnight at 4°C with primary antibodies mouse anti-GFP Living Color (Cell Signaling Technology 2955, 1:1000) to detect TDP-43 YFP and rabbit anti-Beta-Actin (Cell Signaling Technology 4967S ...
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bioRxiv - Cell Biology 2023Quote: ... Embryos were incubated for 1 hr in blocking buffer (PBS-Triton, FBS 10%) then overnight at 4°C with anti-Yap antibody (Cell Signaling, #4912, 1:200) in blocking buffer ...
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bioRxiv - Physiology 2023Quote: ... Neurons were then incubated overnight at 4°C in 1% bovine serum albumin in PBS with pCREB (Ser133) (87G3) Rabbit mAb (1:333, Cell Signaling, Cat No. #9198). Neurons were then thrice rinsed with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... was incubated overnight at 4°C followed by corresponding horseradish peroxidase (HRP)-linked anti-mouse (Cell Signaling Technology, 7076V, 1:2000 dilution in TBST) or anti-rabbit (Cell Signaling Technology ...
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bioRxiv - Microbiology 2023Quote: ... followed by blocking in milk and further overnight probing at 4 °C with a primary antibody specific for Cre (1:500; Cell Signaling Technology, mAb #12830) or GAPDH (1:5000 ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... Cells were incubated overnight at 4° C with rabbit anti-pSMAD1/5/9 (1:600 dilution, Cat: 13820, Cell Signaling Technology, Danvers, MA USA). The secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... and incubated overnight at 4°C with mild agitation in 5% milk in TBST plus indicated antibody: cytochrome c (1:750, rabbit, Cell Signaling Technology, Cat. 11940), GAPDH (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants (Triton X-100 insoluble fraction) were collected and analyzed on 4-20% SDS-PAGE with anti-ubiquitin (Cell Signaling Technologies P4D1, #3936) and anti-Ref(2)P/p62 (Abcam #178840 ...
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bioRxiv - Immunology 2023Quote: ... at room temperature for 1 hour and then incubated overnight at 4°C with primary anti–SYK (1:1000 dilution, Cell Signaling Technology, no. 2712S), anti–p-p38 (1:1000 dilution ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a variety of primary antibodies including anti-MFN2 (Cell Signaling Technology; diluted 1:200), anti-TRA-1-81 (diluted 1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated overnight with the following primary antibodies at 4°C: rabbit anti-Ki67 (1/200; 12202; Cell Signaling Technology, Danvers, MA), rabbit anti-Olfm4 (1/400 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were then precleared by incubation for 1 hour at 4°C while rotating with 50 μL protein A agarose beads (Cell Signaling Technologies, Cat #9863) CCNT1 antibody (Cell Signaling Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were then stained with the appropriate primary antibody diluted in blocking solution and incubated overnight at 4°C: Phospho-Histone H3 (Cell Signaling, #9701, 1:200), Cleaved Caspase 3 (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were processed for Nissl staining (Cresyl violet staining) or immunohistochemistry with antibodies against cleaved caspase-3 (Asp175; Cell Signaling Technology, Inc. Danvers, MA, USA; 1:500) and c-Fos (PC05 ...
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bioRxiv - Bioengineering 2020Quote: ... and 6 μg of pY100 (Cell Signaling Technologies), rotating overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... cl-caspase-6 (1:100, Cell Signaling 9761S), GFAP (1:2,000 AVES GFAP) ...
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bioRxiv - Cell Biology 2023Quote: ... and mouse monoclonal (3675, Lot 6, Cell Signaling); ppMLC2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Whole embryos were fixed in 4% paraformaldehyde overnight and stained with anti-phospho-Smad1/5/9 Rabbit monoclonal antibody (Cell Signaling Technology #13820S; 1:500), anti-phospho-H3 Rabbit monoclonal antibody (Cell Signaling Technology #9701S ...
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bioRxiv - Biochemistry 2020Quote: ... Free-floating sections were blocked with 5% donkey serum for 1.5 hours before overnight incubation at 4°C with primary antibodies (goat anti-Albumin antibody, Abcam ab19194, 1:100 dilution; rabbit anti-V5 antibody, Cell Signaling Technology, 1:500 dilution). Sections were washed ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibody in 10 % Donkey Serum in PBS overnight at 4 °C: Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb (Cell Signaling Technology, Cat# 5364; RRID:AB_10694233). After HSCs were washed three times with PBS + 0.1 % Tween-20 ...
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bioRxiv - Cancer Biology 2022Quote: ... The membranes were blocked with non-fat milk at room temperature for an hour and incubated overnight at 4°C with a primary antibody: anti-P2RX4 (Cell Signaling Technology, Cat# 70659, RRID:AB_2799789) and anti-P2RX7 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at +4°C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474 ...
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bioRxiv - Neuroscience 2022Quote: ... stored as free-floating sections in phosphate buffered saline at 4°C and then incubated with a rabbit antibody against cFos (1:1000, Cell signaling #2250, Danvers, MA, USA) for 48 h ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% dry milk in TBS and incubated overnight at 4 °C with specific monoclonal antibody anti-p-p38 MAPK (Thr180/Tyr182) (1:2000, Cell Signaling, Danvers, MA, USA, 4511) and anti-actin (1:7000 ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were blocked using 5% non-fat milk in TBS-0.05% tween20 (TBST) and incubated overnight at 4 °C with primary antibodies against P-Rex1 and GAPDH (Cell signaling technology #13168 and #5174 respectively). The membranes were washed three times with TBST and then incubated with secondary antibodies in blocking solution for 2 h at room temperature ...