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Citations for Cell Signaling Technology :
1451 - 1500 of 1680 citations for 6 CHLORO 3 METHYLPYRIDO 3 4 D PYRIMIDIN 4 3H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... and blocked with 5% skimmed milk in TBST for 1 h followed by O/N incubation at 4 °C with appropriate primary antibody: pSTAT3 (D3A7; Cell Signaling Technology, 9145S) and STAT3 (124H6 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4°C for 18-22 hr in a cocktail comprising anti-GSK3β mouse monoclonal antibody (1:1500; #9832, Cell Signaling, Rockford, IL), anti-phospho GSK3β (Ser9 ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with hematoxylin-eosin staining for 10min or overnight at 4℃ using the following primary antibodies: β-catenin (1:200, #8480, Cell Signaling Technology), Ki67 (1:400 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with ice-cold 100% (v/v) methanol for 4□min and then incubated with anti-LC3B (3868; Cell Signaling Technology, MA), anti-LAMP-2A (ab18528 ...
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bioRxiv - Plant Biology 2022Quote: ... membranes were incubated overnight at 4°C with the primary antibody in 1 % milk solution in TBS (phospho-AMPK, 1:2000, Cell Signaling Technology, #2535). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at room temperature for 2 hours or overnight at 4°C with the following primary antibodies: Sirt3 (1:200, Cell Signaling Technology 5490S), TOMM20 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... 200,000 Bead-bound cells were incubated with primary antibody overnight at 4°C (1:100 Acetyl-Histone H3 (Lys27) Rabbit mAb (Cell Signaling Technology #8173), 1:50 Acetyl-Histone H3 (Lys9 ...
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bioRxiv - Neuroscience 2023Quote: ... chemically cross-linked to protein G-agarose beads at 4°C on a mini-rotator. Rabbit anti-GFP (TorryPines, cat. # TP401,) or normal rabbit IgG (Cell signaling, cat. # 2729) chemically cross-linked to protein G agarose beads were used as controls ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked with 5% BSA in TBS for 1 hr and then incubated overnight at 4° C with anti-pSMAD1/5/8 (1:1000, Cell Signaling Technology, 13820), anti-SMAD1 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were blocked in 5% BSA with 1% Tween 20 for 1 hour then probed overnight at 4°C with the following primary antibodies at a 1:1,000 dilution: Phospho-STAT1 (Cell Signaling Technologies, 9167S, monoclonal), SOCS1 (Cell Signaling Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked in 5% skimmed milk and incubated overnight at 4°C with primary antibodies for UTX (Cell Signaling Technologies #33510), GFP (JL-8 ...
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bioRxiv - Bioengineering 2023Quote: ... Staining was performed by incubating the cells overnight at 4°C with primary antibodies diluted in 1% BSA targeting α-smooth muscle actin (1:200; 48938S, Cell Signaling Technology), gamma actin (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was incubated overnight at 4°C with the primary antibody mAb c-MYC (dilution 1:1000 in TBST/5% milk; Cell Signaling Technology, D84C12). Subsequently ...
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Suppression of the Integrated Stress Response in Islet β Cells Decreases Risk of Autoimmune DiabetesbioRxiv - Cell Biology 2023Quote: ... The blots were probed with the following primary antibodies and 0.2% Tween20 with overnight incubation at 4°C: anti-p-eIF2α (Abcam; ab32157; 1:1000)(Cell Signaling; 3398s; 1:1000), anti-total-eIF2α (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% non-fat dry milk in TBST for 1 h and then incubated overnight at 4°C in blocking buffer containing the antibody against TMEM106B (1:500, Cell Signaling Technology 93334). The secondary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... or 5% non-fat dry milk in TBST for 1 h then incubated overnight at 4°C in blocking buffer containing antibodies against TMEM106B (1:500, Cell Signaling Technology 93334), TDP-43 (1:1,500 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then immunoblotted over night at 4°C with the indicated primary antibodies diluted in blocking buffer: Phosphorylated Stat1 (Tyr701) (D4A7) (Cell Signaling, 1:1000), STAT1 (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated overnight at 4°C in 0.2% BSA/TBS with the following antibodies: anti-Myc mouse IgG (Cell Signaling, cat. No. 2276) for mDGKε-Myc staining and anti-STIM1 rabbit IgG (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: ... were then added to the membrane and incubated overnight at 4°C: anti-Calnexin (1:1,000 dilution, clone C5C9, Cell Signaling Technology, Danvers, MA), anti-Mitofilin (1:500 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... the membranes were incubated overnight at 4°C with the following primary antibodies: anti-AMPKα (Cell Signaling Technology, USA; diluted at 1:1000), anti-p- AMPKα (Thr172 ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: ... samples were stained with primary antibodies at 4°C overnight at the following dilutions: rabbit anti-Vg (a gift from Sean Carroll and Kristen Guss, 1:200) and rabbit anti-Caspase 3 (Cell Signaling Technology, Cat# 9661, 1:200), mouse anti-beta Galactosidase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with TBS and 3% horse serum and 0.25% Triton X-100 before being incubated with Alexa Fluor© 488 Anti-Rabbit IgG (Cell Signaling, cat#4412S, diluted 1:250) for 2 hours at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Membranes were then washed 3 x 10 minutes in TBS-T and incubated overnight in primary antibodies against phosphorylated Akt T308 (Cell Signaling Technologies #2965; diluted 1:1000), phosphorylated Akt S473 (Cell Signaling Technologies #9271 ...
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bioRxiv - Developmental Biology 2023Quote: ... portions of the entire pancreas were sectioned at 5μm thickness and ≥3 non-consecutive sections of each portion were stained with an antibody against insulin (1:100, Cell Signaling Technologies: 4590, Danvers, MA, USA), which was subsequently detected by biotinylated goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following primary antibodies were diluted 1:1000 in 3% BSA/T-TBS and incubated for about 16 h: anti LC3-I/II (cat. # 12741S) from Cell Signaling Technologies (distributed by Euro-Clone); anti-α-Tubulin (cat ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Cathepsin D (Cell signaling, cat.no. 2284S), and anti-GAPDH (Cell signaling ...
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bioRxiv - Immunology 2021Quote: ... Gasdermin D (Proteintech and Cell Signaling Technologies), caspase-1 (AdipoGen Life Sciences) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cleaved Gasdermin D (Cell Signaling CAT#36425S), and B cell lymphoma (Bcl)-2 (Cat#Ab182858 ...
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bioRxiv - Neuroscience 2023Quote: ... (Cell Signaling Technologies, R&D, Proteintech, Abcam). Only the single banding pattern between 100 kDa-75 kDa was present in ganglia and is therefore used in the validation blot for the antibody and to verify presence in ganglia ...
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bioRxiv - Developmental Biology 2022Quote: ... Pulling down of the pre-cleared chromatin was performed with the use of magnetic beads that were previously incubated with 5 µg of antibody (Histone H3 Lysine 4 trimethylation; H3K4me3; Cell Signaling Technology, cat. #9751) for 8 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sonicated lysates were cleared once by centrifugation and incubated overnight at 4°C with magnetic beads bound with NFκB antibody (Cell Signaling Technology, Cat. No. 8242s) to enrich for DNA fragments ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were blocked in 5% bovine serum albumin (BSA) and incubated overnight at 4 °C with primary anti-phospho-SYK (Cell Signaling Technologies, 1:1000) or anti-SYK (Cell Signaling Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The blots were incubated overnight at 4°C with the primary antibodies: His-Tag (D3I1O) XP® Rabbit mAb (1/1,000 dilution) (Cell Signaling Technology, Danvers, MA) and Myc-Tag (9B11 ...
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bioRxiv - Neuroscience 2021Quote: ... was used at dilution 1:20 and incubated with the cell solution at 4°C for 30 min before staining with AlexaFlour 488 goat anti-mouse (Cell Signaling, 1:100 dilution) for another 30 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Sections were then incubated in rabbit polyclonal anti-αSMA or rabbit monoclonal anti-Runx2 at 4° C overnight (Abcam ab5694, 1:100; Cell Signaling Technology, 1:100). Slides were thoroughly washed the next day then incubated in goat anti-rabbit IgG Alexa Fluor 647 secondary antibody for 1 hour at room temperature (ThermoFisher A-21245 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C with Rabbit anti-KHSRP (1:1000; Novus) or Rabbit anti-GAPDH (1:2000; Cell Signaling Tech, Beverly, MA), Streptavidin-HRP (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated overnight at 4°C in the primary antibodies against c-Fos (1:6400; catalog #2250S; Cell Signaling Technology, Danvers, MA), and then incubated with the secondary Alexa Fluor® 448 (1:800 ...
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bioRxiv - Cell Biology 2021Quote: ... 50μg of chromatin were immunoprecipitated overnight at 4°C with an antibody against Rev-erb-α (13418S, Cell signaling Technology, Danvers, Massachusetts, United States). BSA/yeast tRNA-blocked Protein A/G dynabeads (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then rinsed and blocked with 5% BSA for 1hr prior to overnight incubation (4°C) with monoclonal rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)-XP (Cell signaling, 4511T, 1:1,000) or monoclonal rabbit anti-p38 MAPK (D13E1 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244, 1:800, Cell Signaling Technology, catalog # 5364S), GFP (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and incubation with the primary antibodies in the blocking buffer for overnight at 4°C was followed by a two-hour incubation with secondary antibodies (Cell Signaling, Danvers, MA, USA) in the blocking buffer at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Tween for 1 h and detection was carried out overnight at 4 °C with rabbit anti-phospho-Chk1 (Ser345) mAb (133D3) (Cell Signaling 2348; Lot 15) or rabbit anti-Phospho-Chk2 (Thr68 ...
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bioRxiv - Molecular Biology 2020Quote: ... and subsequently incubated overnight at 4 °C with the primary anti-ADPr-antibody (diluted 1:10’000 in TBS-T; Cell Signaling Technology, Leiden, Netherlands25, 26). Membranes were washed 3x for 5 min with TBS-T and incubated IRDye goat-anti-rabbit IgG secondary antibody (diluted 1:15’000 in TBS-T ...
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bioRxiv - Developmental Biology 2019Quote: ... Blots were probed overnight at 4 deg C with rabbit anti-V5 (Cell Signaling Technologies; 1:1000 in 5% BSA, 0.1% Tween 20, TBS) or for 1 hr at room temperature with mouse anti-β actin (GeneTex ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were separated on a 4–12% SDS-PAGE gel followed by standard immunoblot analysis using anti–CISH (Cell Signaling, Clone D4C10, 1:2000) and Vinculin (Cell Signaling ...