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Citations for Cell Signaling Technology :
1251 - 1300 of 1657 citations for QuantiFluo Caspase 3 Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling, Cat#5656). To remove the non-specific binding ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were blocked with 3% BSA in PBS for 1 hour and cells were stained with VEGFR2 (Cell Signaling; C#2479; 1:200) and EEA1 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Genetics 2024Quote: ... and then fixed at different time points (1 hour, 3 hours, 6 hours and 24 hours) before staining for DNA damage markers: ψH2AX (Cell Signaling Technology # 9718T), 53BP1 ((Novus Biologicals # NB100-904 ...
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bioRxiv - Molecular Biology 2021Quote: Cell proliferation was examined using DNA synthesis analysis (bromodeoxyuridine (BrdU) incorporation assay) or Ki67 detection with specific antibody (Cell Signaling Technology, Danvers, MA) as described previously (7 ...
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bioRxiv - Cancer Biology 2022Quote: Whole cell protein was extracted from cells treated for 24 h with 1 mM aspirin and 4 μM regorafenib using radio-immunoprecipitation assay buffer (Cell Signaling Cat# 9806) supplemented with 1 mM phenylmethanesulfonyl fluoride (Cell Signaling Cat# 8553) ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used for cleavage under targets & release using nuclease (CUT&RUN) assays: anti-H3K27ac (8173, Cell Signaling Technology, 1:100) and anti-H3K4me1 (5326 ...
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bioRxiv - Molecular Biology 2020Quote: Cell were washed twice with phosphate-buffered saline and lysed with sonication in radioimmunoprecipitation assay (RIPA) buffer (#9806, Cell Signaling, Danvers, MA, USA), before being incubated on ice for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Neuroscience 2023Quote: ... cultured primary pericytes were lysed with cold lysis buffer of radioimmunoprecipitation assay (RIPA) lysing buffer supplemented with 1x protease/phosphatase inhibitor (Cell Signaling Technology; U.S.A). Cells were mechanically agitated for 5 minutes and incubated on ice for a further 30 minutes with occasional agitation ...
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bioRxiv - Molecular Biology 2024Quote: All cargo constructs include an epitope tag or tandem tag (FLAG or 3xFLAG) for assay by anti-FLAG primary antibodies (Cell Signaling Technology, 2368). HEK293FT cells were transfected at 24 well ...
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bioRxiv - Neuroscience 2024Quote: ... 96-well Meso Scale Discovery assay plates (MSD, L15XA-6) were coated with 30 μl of pS65-Ub capturing antibody (Cell Signaling Technology, 62802) with a final concentration of 1 µg/ml in 200 mM sodium carbonate coating buffer (pH 9.7 ...
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bioRxiv - Developmental Biology 2021Quote: ... A SignalStain DAB Substrate Kit (Cell Signaling Technology) was used for chromogenic antibody detection ...
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bioRxiv - Cancer Biology 2022Quote: ... Senescence β-Galactosidase Staining Kit (Cell Signaling Technology), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and detected using ECL detection kit (Cell Signaling). A summary of antibodies used is presented in Supplementary Information Table S3.
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bioRxiv - Physiology 2023Quote: ... and cAMP ELISA kit (Cell Signaling Technology, #4339) were used to measure these cyclic nucleotide second messengers from adult A ...
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bioRxiv - Molecular Biology 2020Quote: ... phospho-eIF2α (Ser51) clone D9G8 #3398) or mouse monoclonal antibodies (eIF2α clone D-3, Santa Cruz #sc-133132, β-Actin clone E4D9Z, Cell Signaling Technology #58169 ...
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bioRxiv - Genomics 2020Quote: ... sections were blocked in 3% bovine serum albumin (BSA) and incubated over-night with antibodies recognizing Birc5 (#2808, Cell Signaling Technology, Danvers, MA, USA), Cyclin D1 (#2978 ...
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bioRxiv - Neuroscience 2019Quote: ... Total and phosphorylated glycogen synthase kinase 3 (GSK3-β) were detected using rabbit antibodies anti-GSK3-β (9315) and anti-pGSK3α/β-Ser21/9 (9331) (Cell Signaling). Chemiluminescence signals were analyzed using Image Lab software 6.0 (Bio-Rad).
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bioRxiv - Molecular Biology 2019Quote: ... Tween and detection of total CHK1 or CHK2 proteins was carried out for 1 h at RT using mouse-anti-Chk1 mAb (2G1D5) (Cell Signaling 2360; Lot 3) or mouse anti-Chk2 mouse mAb (BD Biosciences 611570) ...
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... acetyl-histone 3 (Lys27, #8173, D5E4), histone3 (#4499, D1H2), EP300 (# 86377, D8Z4E), and CREBBP (# 7389, D6C5) were purchased from Cell Signaling Technologies (Danvers, MA). An anti-acetyl-SMC3 antibody was a kind gift from Forma Therapeutics ...
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bioRxiv - Biochemistry 2023Quote: ... The membranes were washed with PBS 3 times for 5 minutes and then incubated with horseradish peroxidase conjugated secondary antibody (Cell Signaling Technology, 7074, 7076) in 5% non-fat milk in PBST at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... The expression of arrestin-3 and GPCRs was determined by western blot using anti-arrestin F431 [32] anti-HA (#3724, Cell signaling Technology, Danvers, MA) antibodies.
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Cell Biology 2020Quote: SMC were seeded at a density of 75000/well in 6 well plates and cultured in FGM for 48 h prior to fixation and staining using a commercial β-galactosidase assay (Cell Signaling Technologies #9860) as described previously (41) ...
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bioRxiv - Cancer Biology 2022Quote: The cells were harvested and lysed using radioimmunoprecipitation assay (RIPA) buffer supplemented with PMSF (1mM) and protease inhibitor cocktail (Cell Signaling Technology, Massachusetts, USA). The protein concentration was determined using Bradford protein assay (Sigma ...
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bioRxiv - Immunology 2023Quote: 18 µL U-87 MG EVs that had been separated by UC or SEC were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signaling Technology, catalog #9806) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: SOX2 (goat, R&D, AF2018, 3 μg/mL) and NeuN (rabbit, Cell Signaling Technology, 12943S, 1:500) diluted in antibody blocking solution (1X PBS pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated overnight at 4°C in a primary antibody solution consisting of rabbit-anti-pSMAD1/5/9 (1:600, Cell Signaling Technology #13820 Lot 3) and goat-anti-SOX2 (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 5% milk in TBS/0.1% Tween-20 (TBST) and incubated with anti-phospho-eNOS (Thr495) and anti-eNOS (Suppl Table 3; 1:1,000, Cell Signaling cat. #9574 and 9572, respectively). Membranes were washed in TBST ...
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bioRxiv - Developmental Biology 2023Quote: We used an antibody for phospho histone 3 (pH3) that was previously reported(Shirae-Kurabayashi et al, 2006) (pH3-ser10-6g3-mouse-mAb, Cell Signaling; #9706; 1:500 diluted). The primary antibody was added together with anti-DIG antibody during FISH process ...
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bioRxiv - Immunology 2023Quote: ... Endogenous peroxidase activity was quenched by 3% hydrogen peroxidase prior to 4-hour incubation at 4°C with 5% normal goat serum (Cell Signaling Technology®, Danvers, MA) and rabbit anti-DSG1 (clone EPR677766(B) ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then blocked in 3% hydrogen peroxide solution for 5 minutes and incubated by pSTAT1 Ser727 antibody (Cell Signaling Technology, cat. 8826, 1:200), or pSTAT3 Y705 antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: Senescence β-Galactosidase Staining Kit (9860, Cell Signaling technology) was applied to measure the senescent monolayer cultured neural progenitor cells according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... rabbit anti-Phospho-KIT (Try719) antibody (#3391; Cell Signaling), rabbit anti-Green Fluorescent Protein antibody (AB3080P ...
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bioRxiv - Neuroscience 2020Quote: ... The PathScan®Immune antibody array kit (Cell Signaling) was used according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ChIP was performed using ChIP kit (Cell signaling, 57976s). Briefly ...
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bioRxiv - Immunology 2020Quote: ... ChIP was conducted using SimpleChIP kit (Cell Signaling Technology) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... ER Stress Antibody Sampler Kit (#9956, Cell Signaling Technology), Sec24C (#14676 ...
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bioRxiv - Developmental Biology 2022Quote: ... SimpleChIP plus enzymatic chromatin IP kit (Cell Signaling Technologies) was used for ChIP experiment as per manufacturer recommendations and PCR was used to detect ChIP products run out on agarose gel.
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bioRxiv - Cancer Biology 2023Quote: ... and digested using the PTMScan Kit Protocol (Cell Signaling). Peptide purification was performed using Sep-Pak® C18 columns (Waters) ...
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bioRxiv - Physiology 2023Quote: A competitive cGMP ELISA kit (Cell Signaling Technology, #4360) and cAMP ELISA kit (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... The SA-b-gal staining kit (#9860, Cell Signaling) was performed according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Anti-AhR and anti-5-loxygenase (5-LOX) antibodies (Ab) for immunoblot assay and anti-trimethyl H3K4 (H3K4me3) Ab for chromatin immunoprecipitation (ChIP) were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-ALOX5AP (FLAP ...
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bioRxiv - Developmental Biology 2021Quote: ... HTPCs at P6 and P 15 were seeded onto glass cover slips and stained with a commercial kit (Senescence β-Galactosidase Staining Kit, Cell Signaling Technology) according to the manufacturer’s instructions and as described before(Schmid et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Magnetic beads for all experiments come from PTMScan® HS Ubiquitin/SUMO Remnant Motif (K-ε-GG) Kit (Cell Signaling Technology, Kit #59322), along with 1X HS IAP Bind Buffer #1 and 1X HS IAP Wash Buffer ...