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Citations for Cell Signaling Technology :
1201 - 1250 of 1657 citations for QuantiFluo Caspase 3 Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). Sequencing libraries were prepared from isolated plasmids as for MPRA and sequencing on a MiSeq (50bp ...
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bioRxiv - Immunology 2023Quote: ... with the SimpleChIP Plus kit (Cell Signaling). The ratio of rs2836882 alleles in the PU.1-bound DNA was quantified in duplicate by TaqMan genotyping (assay C 2601507_20) ...
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bioRxiv - Immunology 2024Quote: ... using an immunofluorescence kit (Cell Signaling Technologies). Samples were stained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Single clones were picked out after 3 weeks and Cas9 expression level in each clone was determined by western blotting with Cas9 antibody (Cell Signaling, #14697). Cas9 activity was tested using the CRISPRtest™ Functional Cas9 Activity Kit for Human Cells (Cellecta ...
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bioRxiv - Immunology 2020Quote: ... IL-1β and KC in the cell lysates and the culture supernatants were determined by western blot analysis using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as loading control with specific antibodies (Cell Signaling Technology) as previously described (19).
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bioRxiv - Developmental Biology 2019Quote: ... Embryos/explants were then blocked in PBS+ 10% FBS and 1% Triton X-100 and incubated overnight at 4°C with an anti-pSmad2/3 antibody (Cell Signaling #8828) at 1:1000 in block ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and Cleaved Caspase 3 (#9664, 1:400 dilution for IF) and anti-Rsk2 (#5528; 1:200 dilution for IF) were purchased from Cell Signaling Technology. Anti-Tubulin α (66031-1-Ig ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Microbiology 2019Quote: ... 1:3000 diluted in 1% BSA) and mouse monoclonal anti- HA (6E2) tag antibody (1:1000 dilution in 3% bovine serum albumin, Cell signaling, #2367) for 1hr at RT ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Bioengineering 2022Quote: ... were washed with TBS buffer 3 times for 5 minutes and subjected to antigen retrieval using 1X SignalStain® Citrate Unmasking Solution (Cell signaling). The citrate buffer was bought to boiling temperature and added to the sections ...
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bioRxiv - Cell Biology 2020Quote: ... Galectin-3 (#87985, IF: 1:400) and Na/K-ATPase α1 (#23565, IF: 1:100) antibodies were purchased from Cell Signaling Technology. Anti-beta-actin antibody (A2228 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained overnight at 4°C with AlexaFluor 647-conjugated anti-β-catenin antibody L54E2 at 1:300 dilution supplemented with 3% BSA (2677S, Cell Signaling). Between and after each of these steps ...
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bioRxiv - Cell Biology 2021Quote: ... The following primary antibodies (diluted in 3% w/v BSA in TBST) were used: adipose triglyceride lipase (ATGL) (1:1000, Cell Signaling #2138), perilipin5 (PLIN5 ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Immunology 2022Quote: ... Membranes were washed 3 times with TBST and then incubated with peroxidase-conjugated secondary antibody (Cell Signaling Technology, Cat.No. 7074S, 1:3000). The antigen-antibody reaction was visualized by enhanced chemiluminescence assay (ECL ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Cell Biology 2022Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed in TBS-T (3 × 5 min) and probed with secondary antibodies: mouse (Cat #: 7076S; Cell Signaling Danvers, MA) or rabbit (Cat # ...
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bioRxiv - Neuroscience 2023Quote: ... mouse MsRILP-Flag or rat RtRILP-myc for 3 days and harvested for western blot analysis after lysis in lysis buffer (Cell signaling #9803). Blots were imaged with Li-Cor Odyssey CLx Imager ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Developmental Biology 2023Quote: Immunofluorescence was performed according to the protocol described in the data sheets of the anti-acetylated histone 3 lysine 27 (H3K27Ac) antibody (# 8173, Cell Signaling Technology) and anti-acetylate histone 3 lysine 9 (H3K9Ac ...
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bioRxiv - Cell Biology 2023Quote: ... washed with TBST (3 ×10 min), incubated (1 hr, 20°C) in TBST containing HRP-conjugated secondary antibody (1:3000 anti-mouse, Cell Signaling Technology Cat# 7076S ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-SF3B1 (1:500 dilution; D221-3, MBL Life Science) and anti-phosph-SF3B1(Thr313) (1:500 dilution; D8D8V, Cell Signaling Technology). After washing three times with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then washed 3 times with PBST and were incubated with HRP-conjugated anti-rabbit IgG (1:500 dilution, catalog 7074S, Cell Signaling Technology) for 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated overnight at 4°C with primary antibody detecting light chain 3 type 1 and 2 (Cell Signaling technology, catalog #3868), SOAT1 (catalog #84476) ...
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bioRxiv - Cell Biology 2024Quote: ... Crude cell extract was lysed with an insulin syringe and pre-cleared by incubation with 3 μg Normal Rabbit IgG (Cell Signaling Technology) and 10uL Pierce™ Protein A/G Magnetic Beads (Thermo Scientific 88803 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rabbit, pan, 40D4), phospho-AKT [rabbit, Ser473, (D9E)XP] and GAPDH (glyceraldehyde-3-phosphate dehydrogenasemouse, D4C6R) were purchased from Cell Signaling (USA). Antibodies against human PCNA (proliferating cell nuclear antigen ...
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bioRxiv - Cancer Biology 2024Quote: ... Smad2/3 (#8685S), HIF-1α (#14179S), HIF-2α (#7096S), ASCT2 (#8057) and E-cadherin (#3195S, #14472S) antibodies were obtained from Cell Signaling Technology for immunoblot or fluorescence imaging analysis ...
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bioRxiv - Microbiology 2024Quote: ... For visualization of SARS-CoV-2 infected cells via detection of N protein in Calu-3 cells we used anti SARS-CoV-1/2 Nucleocapsid Mouse anti-N clone (Cell Signaling, 1C7C7) 1:1000 50uL/well.
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bioRxiv - Molecular Biology 2019Quote: 500 μg of unstimulated HT29 lysate was immunoprecipitated onto Protein A/G coated wells (Pierce #15138) following the kinase assay protocol but with 0.5 μg of IKKα (Cell Signaling Technology #2682) or 0.5 μg of naïve rabbit IgG (Jackson Immunoresearch #011-000-002) ...
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bioRxiv - Immunology 2021Quote: ... Western blotting was used as the output assay to determine levels of the ribosomal protein S6 kinase (p-S6K) using an S6K and p-S6K antibodies (Cell Signaling Technology). To assess the impact of mTOR1 inhibition rapamycin (20ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Neuroscience 2021Quote: ... Total protein concentration was determined by a BCA assay (Pierce) 60 μl Protein A beads with 50% slurry (Cell Signaling Technology, #9863S) were then added and incubated for 6 hours to pull down the antibody-protein complex ...
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bioRxiv - Immunology 2022Quote: ChIP assays for histone modifications were performed as described previously (Aoyama et al., 2018) using an anti-H2AK119ub1 (#8240S; Cell Signaling Technology) and an anti-H3K27me3 (#07-449 ...
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bioRxiv - Cancer Biology 2022Quote: ... were used in Western blot assays in xenograft pharmacodynamic (PD) studies: SYK (AB_2687924) (rabbit mAb, Cell Signaling Technology, Cat# 13198, 1:1000), EGFR (AB_2230881 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested at indicated time points and lysed in 1× radioimmunoprecipitation assay (RIPA) buffer (Cell Signaling, Danvers, MA, USA; Cat# 501015489) containing 1× protease inhibitor cocktail (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... Radioimmunoprecipitation assay buffer (RIPA) 10x and the ROCK inhibitor Y-27632 (cat# 13624S) were purchased from Cell Signaling Technologies (Whitby, ON, Canada). BrainPhys™ Neuronal Medium was obtained from STEMCELL Technologies (Vancouver ...
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bioRxiv - Bioengineering 2021Quote: ... The plates were then washed 3 times with PBST before adding 100 μl of TMB substrate solution (Cell Signaling Technology, USA, Catalog: 7004S) to the plates after aspirating out the PBST and was incubated for 25 minutes at room temperature on a plate shaker ...
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bioRxiv - Neuroscience 2020Quote: ... blocked in TBST with 3% BSA for 1 hour at room temperature and incubated in primary antibody solutions (in TBST with 3% BSA) directed against: phosphorylated (pY816, #4168, 1:1000; pY515, #9141, 1:1000; pY706-7, #4621S, 1:1000, Cell signaling technology (CST), Danvers ...
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bioRxiv - Neuroscience 2021Quote: ... the retinal explants were immuno-stained against microtubule with beta-3 tubulin (TUBB3) antibody (1:250, from rabbit, Cell Signaling Technology, 5568S, USA), washed in 0.5% Triton in PBS for 5 times ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then washed with 1× TBST (3 × 10 min) and incubated with anti-mouse IgG horseradish-peroxidase secondary antibody conjugate (1:10000 dilution; Cell Signaling Technology, 7076S) in 1× TBST for 1 h at room temperature ...