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Citations for Cell Signaling Technology :
1201 - 1250 of 1309 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Membrane was blocked using 1x Roti®Block (Carl Roth) for one hour and incubated over night at 4 °C with the primary antibodies against cGAS (Cell Signaling Technology, #31659, 1:1000), β-actin (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... the membranes were incubated overnight at 4°C with gentle agitation in primary antibody dilution buffer: phosphorylated ALK (Y1604) (Cell Signaling Technologies, #3341, 1:1000), ALK (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were detected from the blots using the following primary antibodies diluted in the appropriate ratio with 1x TBST and incubated overnight at +4°C: rabbit anti-phospho-Akt1 (S473, 1:1000, #9018, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-Akt2 (S474 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% dry milk in TBS and incubated overnight at 4 °C with specific monoclonal antibody anti-p-p38 MAPK (Thr180/Tyr182) (1:2000, Cell Signaling, Danvers, MA, USA, 4511) and anti-actin (1:7000 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were dissolved with 3% BSA in tris buffered saline (TBST) and membranes were incubated overnight at 4°C with SQSTM1/p62 (Cell Signaling, cat# 88588, 1:1000). The primary LC3 A/B (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated overnight at 4°C with rabbit anti-cFos antibody (1:6400 dilution, #2250, Cell Signaling Technology, Danvers, MA, USA), 1% NHS ...
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bioRxiv - Immunology 2024Quote: ... and kept at 4 °C for up to 10 days until chromatin fragmentation using SimpleChIP® Plus Sonication Chromatin IP Kit (Cell Signaling Technology, Danvers, MA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Neuroscience 2022Quote: ... stored as free-floating sections in phosphate buffered saline at 4°C and then incubated with a rabbit antibody against cFos (1:1000, Cell signaling #2250, Danvers, MA, USA) for 48 h ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... and probed overnight at 4°C on a shaker with mouse anti-Ty (Bastin et al., 1996) and rabbit anti-HA (Cell Signaling Technologies, cat. no. 3724S). Membranes were then incubated with goat anti-mouse IgG conjugated to HRP (VWR ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 5% goat serum/2.5% BSA for at least 1 hour before overnight incubation at 4°C with an antibody cocktail consisting of rabbit anti-vimentin (1:100) (catalog #: 5741; Cell Signaling Technologies; Danvers, MA, USA), mouse anti-dystrophin IgG2b (1:50 ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked using 5% non-fat milk in TBS-0.05% tween20 (TBST) and incubated overnight at 4 °C with primary antibodies against P-Rex1 and GAPDH (Cell signaling technology #13168 and #5174 respectively). The membranes were washed three times with TBST and then incubated with secondary antibodies in blocking solution for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and then incubated overnight at 4°C with PANX1 CT-412 (Penuela et al., 2007; Penuela et al., 2009) or HA (Cell Signaling 3724, RRID:AB_1549585, Danvers, MA) primary antibody in a humidity chamber ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was incubated overnight at 4°C under rotation with the following primary antibodies diluted in TBST (1:1000): mouse anti-CD44 (Cell Signaling Technology, Boston, MA, USA); rabbit anti-MMP2 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2021Quote: ... washed three times for 10 min in PBS and incubated for 1 hour in blocking solution (5% normal goat serum (Cell Signaling Technology #5425), 0,3% Triton X-100 in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed three times for 5 min with PBS and incubated in anti-mouse IgG Alexa Fluor® 555 Conjugate (Cell signaling #4409) at a dilution of 1:500 for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were washed 4 times in TBS-T for 5 min at room temperature after which they were incubated with HRP-conjugated secondary antibodies (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Histological sections (5 mm) were stained for immunohistochemistry using a standard protocol with antibodies for LC3A/B (1:200; #12741S; Cell Signaling Technology, USA).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed with Bond Wash solution 2x 10s and blocked with a phosphate-buffered saline (PBS) solution containing 5% normal goat serum (5425S, Cell Signaling, Danvers, MA) and 0.5% TritonX-100 (T8787 ...
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bioRxiv - Biochemistry 2021Quote: ... boiled in sample buffer for 5 min and subjected to immunoblot assay with anti-HA antibody (Cell Signaling Technology, Inc., #3724, Danvers, MA).
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitates with anti-lamin A/C antibody were analysed in parallel with 5% unbound supernatants by immunoblotting with anti-lamin A/C (Cell Signaling; Cat# #4777) or anti-His (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... then probed overnight at 4°C with primary antibodies diluted in a 5% BSA/ 1X TBS-0.1% Tween 20 (TBS-T) solution using either Vinculin (E1E9V) (Cell Signaling, Cat. No. 13901) diluted 1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... For live cell microscopy cells were untreated or treated with 5 μM Aβ fibrils (as described above) pre-incubated with an Aβ antibody (Cell Signaling Technologies, #15126) conjugated to an Alexa Fluor 555 secondary (Invitorogen) ...
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bioRxiv - Bioengineering 2022Quote: ... The membranes were blocked with PBS with 0.1% Tween-20 (PBST) with 5% Blotto for one hour and then incubated with antibodies to phospho-p90RSK (Thr359/Ser363; #9344; Cell Signaling Technology, Inc.) and RSK1/RSK2/RSK3 (32D7 ...
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bioRxiv - Physiology 2023Quote: ... was heated at 90°C for 5 minutes and loaded into acrylamide gels before incubated with primary antibodies against catalase (CAT, #14097, Cell Signaling, 1:5000), 3-nitrotyrosine (3-NT ...
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bioRxiv - Cancer Biology 2023Quote: ... Each blot was then washed thrice in 1X TBST for 5 minutes and proteins were detected using either LumiGLO/Peroxide Reagent or SignalFire™ ECL reagent (7003 and 6883, respectively, Cell Signaling Technology) and an iBright™ imaging system (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies diluted in 5% skim milk in TBST: horse anti-mouse IgG (1:20,000, Cell Signaling Technology, cat# 7076), donkey anti-sheep IgG (1:20,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and blocked in room temperature with 5% BSA (HiMedia, India) solution for an hour and incubated with respective primary antibodies [FOXM1 (1:2000) (Cell Signaling Technologies, USA); active + pro Caspase-3 (1:3000 ...
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bioRxiv - Biochemistry 2023Quote: The antibodies used in this study for immunoblotting (Figure 5) were (1) GST-Tag monoclonal antibody 26H1 (Cell Signaling Technologies catalog number 2624S); (2 ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were washed three times for 5 min each with TBS-T then incubated in SignalFire ECL Reagent (Cell Signaling Technology, 6883) for 1 min and imaged using a BioRad ChemiDoc imager.
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bioRxiv - Physiology 2024Quote: ... The PVDF membrane was incubated with 5% w/v non-fat dry milk or 5% BSA for 1 h at room temperature and immunoblotted with anti-HSL (phospho-serine 660) (1:1000, Cell Signaling Technology, 45804S), anti-HSL (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... and blocked with 1x TBST + 5% normal goat serum (NGS) cocktail for 60 min at RT before applying the NFκB primary antibody (Cell Signaling, 6956, 1:1000) in 1x TBST cocktail containing 1% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were washed 3×5 minutes in TBST and incubated for 1 hour with HRP-conjugated anti-rabbit IgG (diluted 1:2000 v/v in TBST with 5% BSA; Cell Signaling, cat #: 7074). Membranes were finally washed 3×5 minutes in TBST ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked with PBS-Milk 5% for 30 min at RT before staining with primary antibody against HA-tag (C29F4, Cell signaling, 1:500) and Actin (ab49900 ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed cultures were permeabilized with 0.1% Triton-x in PBS 10 minutes and blocked in blocking buffer (5% Normal Goat Serum (Cell Signaling Tech. cat#: 5425S) in 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking was performed with 5% non-fat milk dissolved in TBST for 1 hour followed by incubation with primary antibodies PRKN (Cell Signaling Technology, 2132) at 1:2000 dilution in 5% BSA in TBST at 4 °C overnight and VCL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins of interest were analyzed using primary antibodies (1:1000 in 5% BSA in TBS-T) as follows: anti-LKB1 (Cell Signaling Technology; 3050S), anti-phospho-LKB1 (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was then blocked for 1hr in 5% skim milk in TBST and incubated in primary mouse monoclonal antibody Lamin A/C 4C11 (Cell Signaling Technology #4777) in 5% BSA overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: Membranes were then incubated 24-48 hours at 4°C in primary antibodies – PERK (1:500 anti-Rbt; Cell Signaling Technologies [CST], Danvers, MA; cat # 3192S), IRE-1α (1:500 anti-Rbt ...
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bioRxiv - Neuroscience 2021Quote: ... The immunodetection of selected proteins was performed overnight at 4 °C with the following antibodies: p62 (Cell Signaling Technology Inc, Danvers, MA, USA, 5114, 1:1000), LC3b (Abcam ab51520 ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C with anti-Cd9 ([1:3000; System Biosciences, Palo Alto, CA, USA] and anti-Gapdh [1:5000; Cell Signaling, Davers, MA, USA; 14C10]).