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Citations for Cell Signaling Technology :
851 - 900 of 1309 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Aortae were perfusion-fixed in situ with 4% paraformaldehyde (overnight) prior to staining with primary beta-catenin antibody (Cell signaling: 9562) and fluorophore-conjugated secondary antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4 % PFA at passage 1 and stained for the endothelial markers CD31 (Cell Signaling 3528S, 1:800), VE-cadherin (2500S ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with the different antibodies overnight at 4 °C (anti-hSYK #2710, anti-hPhospho-SYK #2712 from Cell Signaling were used according to manufacturer’s instructions) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... in Tris-buffered saline with Tween-20 (0.1%), and subsequently incubated (overnight, 4 °C) with primary antibodies for p-Akt (S473) (1:1000, Rabbit mAb, Cell Signaling, 4060S), Akt (pan ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 0.1% Tween 20 (TBST) and probed with primary antibodies overnight at 4 °C using rabbit NLRP3 (1:1000, D4D8T-15101, Cell Signaling), mouse caspase 1 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... 150 to 250 µg of chromatin were incubated overnight on a rotator at 4°C with Ezh2 antibody (Cell Signaling Technology). On the next day ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated overnight at 4° C with the following primary antibodies: rabbit anti-GluA1 ATD (Cell signaling, #13185, 1:500), guinea pig anti-GluA2 (Synaptic Systems ...
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bioRxiv - Genomics 2024Quote: ... 0.05% Tween-20) and incubated overnight at 4°C with primary antibodies: rabbit anti-HIS tag (D3I10) mAb (12698, Cell Signaling); mouse anti-β-tubulin (clone E7 ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h at room temperature and then incubated overnight at +4°C with primary antibody: anti-Snai1 (Cat# 3879, Cell Signaling), anti-STIM1 (Cat# 4119 ...
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bioRxiv - Cell Biology 2024Quote: ... rocking at 4°C (1:1000 rabbit anti-GFP and 1:500 mouse anti-EEA1, or 1:1000 mouse anti-GFP (Cell Signaling) and 1:1000 rabbit anti-GM130 ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then blocked in 5% normal goat serum/0.1% saponin for 1 hr at room temperature and incubated with combinations of the indicated primary antibodies overnight at 4°C: rabbit monoclonal anti-ECAD (clone 24E10; 1:1,000; Cell Signaling Technology), mouse monoclonal anti-HLA-G (clone 4H84 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μg of digested chromatin samples was treated with antibodies against rabbit IgG (1:100; Cell Signaling Technology, 2729) or NKX2.1 (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked with 5% milk powder in TBST for 1 h and probed with SETD2 (Cell signaling, 80290) 1:1000 overnight at 4°C with agitation ...
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bioRxiv - Systems Biology 2020Quote: ... then incubated with anti-vimentin antibody (clone D21H3, Cell Signaling Technologies, (1:500 dilution in 5% milk/TBS-T) and with anti-GAPDH antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were blocked with 5% powdered milk in buffer and probed with pan-SynGAP (1:1,000, #5539, Cell Signaling) or SynGAP-α2 (abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was then incubated with 1× TBST containing 5% milk and either anti-RPS6KB1 (Cell Signaling Technology, 2940S) or anti-DNAJC3 (Cell Signaling Technology ...
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bioRxiv - Pathology 2022Quote: ... Membranes were stained with Ponceau-S and blocked for 30 minutes with 5% nonfat dry milk (Cell signaling, 9999) or 5% BSA in Blotto buffer (0.15M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies were used: Phospho-Smad1/5 (Ser463/465) (41D10) (1:300, Rabbit, Cell Signaling Technology #9516S), P-Histone H3 (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Rabbit polyclonal antibodies to Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) (Cell Signaling Technology, 4668S, 1:2500 in 5% BSA); Rabbit polyclonal antibodies to p38 MAPK (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: EMT was induced in the MCF10A cell line by chronic stimulation with 5 ng/ml TGFβ1 (Cell Signaling Technology) for eight days22 ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was incubated with anti-NTMT1 (1:1000 dilution in 5% BSA in TBST buffer, Cell signaling #13432) at 4 °C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... The PVDF membranes were blocked with 5% BSA and incubated with rabbit mTOR (1:1000, Cell Signaling Technologies, USA), rabbit p-mTOR (1:1000 ...
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bioRxiv - Genomics 2024Quote: ... The membrane was blocked with 5% milk and probed using either 1:1000 antiV5 antibody (Cell Signaling Technology, 13202) or 1:10000 GAPDH as a loading control (Proteintech 60004-1) ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% skimmed milk in Tris-Buffered Saline with Tween 20 (TBST; Cell Signaling Technology) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were first incubated with blocking and permeabilization buffer (1X PBS / 5% normal goat serum (Cell Signaling, cat #5425) / 0.3% Triton X-100 ...
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bioRxiv - Genomics 2023Quote: ... 1ml (5*106 cells) was aliquoted into each of 3 tubes with antibody (1.25 µg anti-HA, Cell Signaling #3724S ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blotted overnight with primary antibodies in TBS-T containing 5% BSA (Cell signaling antibodies; Stat1 (9172), phospho-Stat1-Tyr701-(7649) ...
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bioRxiv - Cell Biology 2023Quote: Cullin-RING E3 ubiquitin ligases were inhibited by addition of 5 μM MLN4924 (Cell Signaling Technology, catalog no.: 85923) and the 26S proteasome was blocked with 10 μM MG132 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative PCR (qPCR) analysis was performed on a QuantStudio™ 5 Real-Time PCR System (Cell Signaling Technology, MA) using the SYBR green reaction mix (Vazyme Biotech ...
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bioRxiv - Bioengineering 2023Quote: ... anti-pSmad 1/5/9 (ref: 13820S) and anti-pSmad 2 (ref: 18338S) antibodies were obtained from Cell signaling. The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked in 5% BSA TBST and incubated in the primary antbodies anti-INSR (Cell Signaling Technology, #3025), anti-TdTomato (SICGEN ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rinsed 3 times with PBS for 5 minutes before adding fluorescent secondary antibody (Cell Signaling Technology 4413S) diluted 1:2000 in PBS containing 1% BSA and 0.1% Triton-X at 37°C for 25 minutes ...
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bioRxiv - Immunology 2024Quote: ... blocked in BSA 5% in TBS-Tween and then stained with antibodies (488-conjugated rabbit anti-Iba1, Cell Signaling, #20825 ...
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bioRxiv - Immunology 2024Quote: ... and subsequently blocked with 5% non-fat milk or BSA and probed with primary antibodies from Cell Signaling Technologies against phospho-proteins p-Akt (Ser473 ...
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bioRxiv - Neuroscience 2024Quote: ... After blocking tissue with 5% swine serum (Jackson Immuno Research, # 014-000-121) and 2% BSA (Cell Signaling, #9998S) in permeabilizing conditions (0.1% Triton X-100 ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary HRP-linked antibody was incubated (anti-mouse, Cell Signaling Technology, 1:3000 in 5% milk in PBS-T) for 1h at RT ...
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bioRxiv - Bioengineering 2020Quote: ... microchannels were incubated overnight at 4°C with anti-vascular endothelial-cadherin (VE-cadherin) and anti-epithelial cadherin (E-cadherin) (Cell Signaling Technologies) and primary antibodies were diluted with blocking buffer for HUVECs and BeWo cells ...
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bioRxiv - Cancer Biology 2021Quote: ... The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100; Cell Signaling Technology), a rabbit polyclonal anti-p-AKT antibody (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 h at room temperature followed by incubation overnight at 4°C with a specific primary antibody to p-p53ser15 (Cell Signaling, 9284S), β-actin (Cell Signaling ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Genetics 2021Quote: ... were incubated overnight at 4 degrees Celcius in a 1:1000 dilution with the following primary antibodies: anti-TP53 (Cell Signaling, 2524), anti-BRCA1 (Abclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde and processed for immunostaining by standard methods using mouse anti-Flag (9A3, Cell Signaling, 1:400), rabbit anti-Myc (71D10 ...
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bioRxiv - Molecular Biology 2021Quote: ... and Tween20 (0.02%)) for 20 minutes at RT followed by treatment overnight at 4 °C with anti-γ-H2AX (Cell Signaling #9718S) diluted 1:500 in Ab mix or anti-PCNA diluted 1:1000 (DAKO #M0879) ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight in blocking buffer at 4°C with the following primary antibodies: 1:2000 c-fos (Cell signaling technology), 1:2000 ds-red (Takara Bio) ...
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bioRxiv - Genetics 2020Quote: ... followed by overnight incubation with primary antibodies overnight at 4°C (anti-dS6K gift from Thomas Neufeld; dilution at 1:3500, anti-pS6KThr398 Cell Signaling Technologies, #9209 ...
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bioRxiv - Cancer Biology 2020Quote: ... cells grown on chamber microscope slides were fixed in 4% paraformaldehyde and treated with immunofluorescence blocking buffer (catalog #12411, Cell Signaling Technology). Staining with primary antibodies against involucrin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocked using 5% milk in TBST for 1 hour followed by primary antibody incubation at 4°C for overnight using the following antibodies at the appropriate dilutions– LC3 (1:1000, #2775, Cell Signaling Technologies), Atg5 (1:1000 ...